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Using data from the regional broadband dense temporary array deployed by the ChinArray project, we applied the three-dimensional (3D) Kirchhoff migration method of the teleseismic P-wave receiver function to investigate discontinuity structures of the mantle transition zone (MTZ) in the central and western parts of the North China Craton (NCC) using a high-resolution 3D velocity model of the East Asian region. The results show that the 410-km discontinuity beneath the Datong Volcano is depressed by ∼10 km, indicating the presence of a high-temperature anomaly near the depth of 410 km, which is likely related to small-scale mantle upwelling caused by the dehydration of the stagnant Pacific Plate in the MTZ. The upwelling of hot material provides a heat source for surface magmatic activity. Beneath the Bohai Bay Basin, significant subsidence of the 660-km discontinuity is observed, and the transition zone here is extensively thickened. It's suggested that the anomalies in this region are related to the stagnation of the Pacific slab in the MTZ. Although the thickness of the transition zone west of the North-South Gravity Lineament appears normal, we propose that the subducting front of the Pacific slab did not cross the gravity lineament in the NCC. In comparison, the small-scale subsidence of the 660-km discontinuity and the thickening of the MTZ observed north of the Hannuoba Volcano likely indicate that the slab crossed the gravity lineament at its turning point and remained in the MTZ. Furthermore, a local thickening of the MTZ is observed in the Dabie orogenic belt of the Qinling Mountains. This is believed to be a combined effect of lithospheric delamination into the transition zone in the lower Yangtze region and the stagnation of the Pacific Plate. [ABSTRACT FROM AUTHOR]

The characterization of dissolved organic matter (DOM) is important for better understanding of the migration and transformation mechanisms of DOM in water bodies and its interaction with other contaminants. In this work, fluorescence characteristics and molecular compositions of the DOM samples collected from the mainstream, tributary, and sewage outfall of the Inner Mongolia section of the Yellow River (IMYR) were determined by using fluorescence spectroscopy and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS). In addition, concentrations of potentially toxic elements (PTEs) in the relevant surface water and their potential relationships with DOM were investigated. The results showed that the abundance of tyrosine-like components increased significantly in downstream waters impacted by outfall effluents and was negatively correlated with the humification index (HIX). Compared to the mainstream, outfall and tributaries have a high number of molecular formulas and a higher proportion of CHOS molecular formulas. In particular, the O5S class has a relative intensity of 41.6% and the O5-7S class has more than 70%. Thirty-eight PTEs were measured in the surface water samples, and 12 found above their detective levels at all sampling sites. Protein-like components are positively correlated with Cu, which is likely indicating the source of Cu in the aquatic environment of the IMYR. Our results demonstrated that urban wastewater discharges significantly alter characteristics and compositions of DOM in the mainstream of IMYR with strongly anthropogenic features. These results and conclusions are important for understanding the role and sources of DOM in the Yellow River aquatic environment. [ABSTRACT FROM AUTHOR]

Due to a lack of toxicity reference values that match the regional environmental characteristics, the ecological risk of metals in water bodies cannot be accurately assessed. The Fen River is the second-largest tributary of the Yellow River in China, and the sustainability of this area is threatened by heavy metal pollution caused by intensive industrial and agricultural activities. In this study, site-specific water quality criteria (WQCs) for heavy metals in the Fen River were derived considering toxicity data from native aquatic organisms and regional water quality factors (e.g., water hardness). Short-term WQCs for Mn, Cu, Cd, Zn, Cr, Pb, and Ni were 2026.15, 98.62, 10.02, 63.07, 6.06, 166.74, and 132.73 μg/L, respectively, and long-term WQCs were 166.53, 29.71, 2.18, 19.29, 4.15, 6.38, and 14.76 μg/L, respectively. The distribution characteristics of these metals during the wet season in 2020 were explored, and their average concentrations in the river water did not exceed the environmental quality standards for surface water in China but were higher than the world average levels. Cr was the main pollutant in the sampling sites of Yaodu region, Hongdong Shitan, Xiao River, and Duanchun River, as was Pb in Duanchun River. Based on the site-specific WQCs, using hazardous quotient (HQ) and margin of safety (MOS10) approaches, a high risk of Pb was identified in the Duanchun River, and a medium risk of Cr might occur at midstream and downstream of Yaodu and Xiaodian. The results will provide a reference basis for heavy metal pollution control and water quality management in the Fen River. [ABSTRACT FROM AUTHOR]

Extensive use of the herbicide glyphosate leads to a high detection rate in the environment and potential risks to nontarget aquatic life. China ranks first globally in the production and consumption of glyphosate, but there are no glyphosate water quality criteria (WQCs) for protecting aquatic life. Here, data on the acute and chronic toxicity of glyphosate and glyphosate-based formulations (GBFs) to freshwater aquatic life were collected and screened. Significant differences in species sensitivity distributions (SSDs) and toxicity values for acute or chronic toxicity were found between glyphosate and GBFs. The hazardous concentrations for 5% of species (HC5) of glyphosate or GBFs between native and nonnative species were different, and native species were found to be more sensitive to the toxicity of glyphosate. The acute and chronic WQCs derived with the SSD method for glyphosate based on the toxicity data for native species in China were 3.35 and 0.26 mg/L, respectively, and those found for GBFs were 0.21 and 0.005 mg/L, respectively. The WQCs in this study were quite different from those estimated using similar statistical extrapolation methods in other countries, which reflects the differences in species sensitivity to glyphosate toxicity in different regions. The hazard quotients (HQs) were calculated based on the WQCs and concentrations of glyphosate in some surface waters in China and indicated that glyphosate exhibits medium or high hazard risk in some samples of Tai Lake, surface water in Guiyang, fishpond water in Chongqing, rural drinking water, and surface water and reservoir water in Henan Province. The WQCs of glyphosate and GBFs have scientific significance for the exposure and pollution control of herbicide formulations and the protection of aquatic life in China. [ABSTRACT FROM AUTHOR]

Sport trade frictions have continued to evolve and escalate, which has a great impact on sport academic cooperation. In order to objectively assess the impact of sports scholarship on China and provide evidence to support future changes in sports academic cooperation, this study takes 269,647 academic papers produced by sports alone from 2010–2018 as the research object and integrates explicit, implicit, and performance information contained in the paper output to construct a multidimensional matrix assessment framework. The horizontal dimension splits the collaborative research output into three mutually exclusive subsets: China-led collaborative research, sport-led collaborative research, and bisectional collaborative research; the vertical dimension systematically analyzes the characteristics of collaborative sport academic research in terms of participants, research content, and research level. The purpose of this study is to characterize the role and status of academic cooperation between the two countries through a long period, large sample, and multidimensional perspective, to make an objective assessment of the impact of academic cooperation between the two countries, and to provide evidence to support a reasonable response to the impact of changes in the relationship between the two countries on academic cooperation. [ABSTRACT FROM AUTHOR]

Polycyclic aromatic hydrocarbons (PAHs) are typical toxic organic pollutants that can accumulate in sediments and may be toxic to aquatic organisms. In the present study, the contamination level, composition pattern, and sources of sixteen PAHs listed by the United States Environmental Protection Agency were investigated in surface sediments and a sediment core from Daihai Lake, which is located in a typical semiarid area of Inner Mongolia, China, and the ecological risk of these PAHs was assessed. The results show that the total concentration of PAHs in the surface sediments ranged from 204.6 to 344.5 ng/g with an average value of 287.2 ng/g and that compared with other aquatic systems, the level of PAHs in the sediments from Daihai Lake was low. However, a general upward trend was observed for the concentrations of PAHs in the sediment core, which might be related to the increase in human activities in the area. Moreover, the PAH concentrations were significantly positively correlated with the total organic carbon (TOC) content in the sediments, and it is thus inferred that TOC regulates the distribution of PAHs in Daihai Lake. Three-ring and four-ring PAHs were found to be predominant in all the sediment samples, and phenanthrene (Phe) was the most abundant compound. According to the composition of PAHs and the anthracene (Ant)/(Ant+Phe) or fluoranthene (Flt)/(Flt+pyrene (Pyr)) ratios, the PAHs in Daihai Lake mainly originated from the combustion of domestic coal, grass, and wood, and petroleum cannot be ignored as a source considering the growth of industry. Risk assessment based on a comparison of PAH concentrations and the effect range low (ERL) and effect range median (ERM) values demonstrated that acenaphthene (Ace) at 11 sites and fluorene (Flu) at 7 sites had occasional adverse biological effects. [ABSTRACT FROM AUTHOR]

Changbaishan (CBS) is the largest intraplate volcano in Northeast China, located far to the west of the Pacific Plate subduction trench. Two end-member models for CBS volcanism differ significantly in the detailed structures of the mantle transition zone (MTZ). The source of the CBS volcanism is still debatable. Diagrams of P - and S -wave velocity variations down to 800 km depth were made by using joint P and S inversion for relative traveltime residuals measured from waveform data recorded by temporary and permanent seismic stations. A broad low-velocity (low-V) zone in the upper mantle was highlighted directly beneath the CBS. Two prominent low-V branches connected to the broad low-V zone: one east of the CBS extending down to the top of the MTZ, the other west of the CBS ranging down to the base of the MTZ. This indicates two deep sources feeding the CBS volcano. A weak high-V zone in the MTZ was also highlighted directly beneath the CBS volcano, possibly the pond of slab fragments. The eastern low-V branch may be related to slab dehydration while the western low-V branch outlines the upwelling conduit in the MTZ. Thus, our proposed dynamic model for the CBS volcanism suggests that the CBS volcano has two deep sources: one from dehydration of the flat-lying Pacific slab and the other from upwelling, likely emerging from the top of the lower mantle. [ABSTRACT FROM AUTHOR]

This study aimed to analyse the pathogenic spectrum and epidemiological characteristics of infectious diarrhea in Yantai City, Shandong Province, China and provide a reference for its prevention and control. A total of 713 stool specimens collected within 3 days of diarrhea onset from January to December 2017 at secondary or higher hospitals in Yantai City were tested for 10 causative pathogens, using real-time polymerase chain reaction (RT-PCR). The top two rotaviruses and norovirus were analysed for typing and geographical distribution. The total positive rate was 46.56% (332/713), and 268 of 713 specimens contained at least one pathogen; 64 had at least two pathogens, accounting for 19.28% of the positive specimens (64/332). The positivity rates of rotavirus (RV), norovirus (NoVs) GI, norovirus (NoVs) GII, enterovirus universal (EV), enteric adenoviruses (EAdV), sapovirus (SaV), astrovirus (Astv), Salmonella (SE), Listeria monocytogenes (LiMo), and Vibrio parahaemolyticus (VP) were 20.06% (143/713), 1.82% (13/713), 12.84% (89/713), 10.66% (76/713), 4.07% (29/713), 0.42% (3/713), 2.38% (17/713), 1.54% (11/713), 1.82% (13/713), and 1.54% (11/713), respectively. Infectious diarrhea showed a high prevalence in young children aged 1-5 years, accounting for 48.6% of the total number of cases. Bacterial diarrhea was predominant in summer, and viral diarrhea was distributed throughout the year, without a significant seasonal pattern. Rotavirus is dominated by G9P, accounting for 81.82%, while norovirus is dominated by the GII type and has diverse characteristics. The aetiology of infectious diarrhea in Yantai is mainly viral, with RV, NoVs, EV, EAdV, and Astv being the most frequent pathogens. Continuous surveillance of infectious diarrhea diseases can help us understand its epidemiological and pathogenic characteristics, thereby taking targeted preventive and control measures in different seasons. [ABSTRACT FROM AUTHOR]

The residues of organochlorine pesticides (OCPs) in surface waters from Meiliang Bay, Gonghu Bay, and Xukou Bay of Lake Taihu, China, were investigated, and their ecological risks were assessed using the risk quotient method and probabilistic risk assessment. Environmental concentrations of OCPs in surface water of these bays were relatively lower compared with other rivers or lakes in China. Calculation of risk quotient associated with taxonomic groups indicates moderate ecological risks from OCPs for crustaceans and insects in these bays, while the ecological risks were low for fish and negligible for phytoplankton. The ecological risk quotients associated with individual OCPs were lower than 0.01 in these bays, suggesting a negligible risk to aquatic organisms. Ecological risk from α-HCH was relatively lower compared with DDTs, endosulfans, and γ-HCH. The combined ecological risks were evaluated using probabilistic risk assessment for only eight OCPs owing to a lack of available toxicity data for β-HCH and δ-HCH. The percentage of species with the potential to be at risk from mixture of OCPs was lower than the criteria of 5% in each bay, indicating that the combined ecological risks were acceptable. [ABSTRACT FROM AUTHOR]

Lake Taihu provides vital ecological services for humans in China; it receives a great deal of attention regarding its ecological and environmental conditions. In this study, the ecological risks of eight individual polycyclic aromatic hydrocarbons (PAHs) in water were assessed using probabilistic distributions of the hazard quotient based on Monte Carlo simulation. The results show that the 95th percentile of the hazard quotients ranged from 0.00074 to 2.831, and the ecological risk of Flua was highest, followed by, in descending order of risk, B[a]P > Pyr > Ant > Phe > Flu > Ace > Chr. The probabilities of hazard quotients exceeding a decision criteria of 0.3 were 18.09%, 6.51%, 3.76%, and 2.85% for Flua, B[a]P, Pyr, and Ant, respectively, indicating their potential ecological risks to aquatic organisms. The spatial distribution of hazard quotients for these four individual PAHs with potential ecological risk were obtained using Geographic Information System (GIS), and similar spatial distribution patterns were also observed in the lake. The highest ecological risks of these four individual PAHs to aquatic organisms were found in Meiliang Bay, followed by Gonghu Bay and Xukou Bay. The uncertainty within the ecological risk assessment was also discussed. [ABSTRACT FROM AUTHOR]

The concentrations of PAHs in the surface waters from seven river basins in China were summarized from literature published from 2000-2010. Subsequently, the risks from individual PAHs and ΣPAHs in the surface waters of China were quantified by comparing the overlap between the probability distributions of exposure concentrations with the probability distributions of toxicity data. The results show that the concentrations of 15 PAHs in the surface waters ranged from 3.09 to 38139.00 ng L, with a geometric mean of 474.93 ng L. The significantly higher concentrations of the 15 PAHs occurred in the surface waters from northern China when compared with those from southern China. The concentrations of the 15 PAHs in Chinese surface waters were higher than those in other waters worldwide. The MOS (margin of safety) values were calculated at 90th percentile values of exposure concentrations and 10th percentile values of toxicity data, with 5.75, 0.17, 2.33, 0.30, 0.57, 1.74, 1.05, and 0.11 for Ace, Ant, Flu, BaP, Flua, Nap, Phe, and Pyr, respectively. The probabilities of the individual PAH concentrations exceeding the 10th percentile value of the toxicity data were 6.06%, 2.07%, 9.51%, and 2.07% for Nap, Ace, Phe, and Flu, respectively, suggesting minimal risk to aquatic organisms; however, the probabilities of BaP, Ant, Flua, and Pyr exceeding this value were 19.49%, 25.46%, 15.15%, and 30.50%, respectively, indicating potential risks. Among the individual PAHs, the ecological risk from Pyr was the highest, followed by, in descending order of risk, Ant, Bap, Flua, Phe, Nap, Flu, and Ace. Additionally, the combined ecological risk of ΣPAH8 in Chinese surface waters was significantly higher than any that of individual PAHs alone. The MOS values in the river basins were < 1, except for the Haihe River Basin, suggesting a potential combined risk from ΣPAH8 in the other six river basins. The probabilities calculation indicate that low to high ecological risk from ΣPAH8 for all aquatic species was reported in the Liaohe River Basin (65.58%), Huaihe River Basin (57.15%), Songhua River Basin (46.49%), Pearl River Basin (38.41%), Yangtze River Basin (25.98%), Yellow River Basin (15.92%), and Haihe River Basin (5.22%). [ABSTRACT FROM AUTHOR]

Triplicate waveform modeling is used to resolve SH ( Vs) and P ( Vp) wave velocity structures in the upper mantle transition zone (TZ) beneath northwestern (NW) Tibet. Focal depth move out stacking is proposed to enhance the identification of triplicate phases, and can be used to test consistency of our data. Our results show that the Vs and Vp structures are decorrelated, and that a large Vs jump occurred across the 660-km discontinuity, with a small Vs gradient above it. Conversely, the Vp model is characterized by a relatively small contrast across the discontinuity, accompanied by a high Vp gradient in the TZ. There seem no significant depth anomalies of the 660-km discontinuity in both models. The seismic structures in TZ beneath NW Tibet are similar to recent studies beneath the central Qiangtang and western Lhasa terrains. Taking the lower TZ structures under India as references, Vs is normal but Vp appears slightly high, and thus a high ratio of Vp/ Vs was indicated beneath NW Tibet. Combined results with experiment information from mineral studies, we suggest that the differential anomalies of Vp and Vs can be attributed to a chemical heterogeneity, such as increased Al content in the lower TZ. Considering the tectonic evolution of Tibet, the chemical heterogeneity may be associated with subduction or detachment of the Tethys oceanic slab. [ABSTRACT FROM AUTHOR]

Selenium (Se), a trace element, is essential for biological growth; however, excess Se in water can harm aquatic organisms, including fish. Thus, increasing attention is being paid to the risk of Se in aquatic ecosystems. However, there has been little research regarding the protection of aquatic organisms from excess Se in China. To comprehensively ensure China's ecological safety, research on the water quality criteria (WQC) of Se is urgently required. Herein, water and fish samples were collected from Taihu Lake and Yellow River estuary to investigate Se distribution characteristics and bioaccumulation factors (BAFs) in aquatic ecosystems. Tissue-based toxicity data and BAFs were used to derive the WQC for Se. Quotient and probabilistic methods were used to assess the ecological risk of Se to aquatic life. The results revealed that the average levels of Se in the water samples were 1.54 and 1.07 μg/L and that the average Se levels in the fish samples were 0.87 and 0.66 mg/kg in Taihu Lake and the Yellow River estuary, respectively. Furthermore, the calculated BAF for Se in fish was 618.69 L/kg, and the calculated WQC for Se to protect aquatic organisms was 0.29 μg/L. The high levels of ecological risk associated with Se in Taihu Lake and the Yellow River estuary indicate that the aquatic risk of Se warrants further attention. This study provides scientific guidance for setting Se water quality standards to protect aquatic organisms and ensure environmental management in China. [ABSTRACT FROM AUTHOR]

Abstract: We present shear wave splitting results obtained from the analysis of teleseismic SKS, SKKS and PKS phases recorded by 70 permanent seismographic stations located in the Northeastern Tibetan Plateau. We identify a contrast in the splitting pattern complexity beneath different parts of NE Tibet. In the western and northern part, anisotropy observations are well explained by a single layer of anisotropy with a fast anisotropic direction trending NWW–SEE or NW–SE. In Xining and its adjacent area, the anisotropy shows strong azimuthal dependence of splitting parameters that can be modeled by two anisotropic layers. The fast direction for the upper layer lies in the N75–95°E range, which is consistent with the surface movement direction determined from GPS, and could be associated with middle to lower crustal flow. The fast direction in the lower layer is in the N105–125°E range and similar to the direction observed in the western and northern part where only a single layer is required. These NWW–SEE or NW–SE fast feature could be related to the current orogenesis induced from the India–Eurasia collision, or flow in the asthenosphere related to the absolute motion of Eurasia. Comparison between the anisotropy patterns expected from proposed models with our shear wave splitting observation suggests that no unique geodynamic model can reconcile all splitting measurements for such a complex region. [Copyright &y& Elsevier]

Background: The COVID-19 pandemic underscored the critical importance of biosafety in microbiology laboratories worldwide. In response, China has ramped up its efforts to enhance biosafety measures within its Centers for Disease Control and Prevention (CDC) laboratories. This study provides the first comprehensive assessment of biosafety practices across provincial, city, and county levels of CDC microbiology laboratories in China., Methods: We conducted a nationwide cross-sectional survey from 2021 to 2023, targeting staff from microbiology laboratories within CDCs at all administrative levels in China. Stratified sampling was employed to select respondents, ensuring a representative mix across different CDC hierarchies, job titles, and academic qualifications. The survey encompassed questions on biosafety training, the presence of BSL-2 and BSL-3 laboratories, adherence to general biosafety guidelines, and management practices regarding specimens, reagents, and consumables. Statistical analysis was performed to identify significant differences in biosafety practices among different CDC levels., Results: A total of 990 valid responses were received, highlighting a nearly universal presence (98.69%) of BSL-2 laboratories and a significant yet varied presence of BSL-3 laboratories across the CDC network. The survey revealed high levels of biosafety training (98.69%) and adherence to biosafety protocols. However, challenges remain in the consistent application of certain safety practices, especially at lower administrative levels. Notable differences in the management of specimens, reagents, and consumables point to areas for improvement in ensuring biosecurity., Conclusion: Our findings indicate a robust foundation of biosafety practices within CDC microbiology laboratories in China, reflecting significant advancements in the wake of the Biosecurity Law's implementation. Nevertheless, the variability in adherence to specific protocols underscores the need for ongoing training, resources allocation, and policy refinement to enhance biosafety standards uniformly across all levels. This study's insights are crucial for guiding future improvements in laboratory biosafety, not just in China but potentially in other countries enhancing their public health infrastructures., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Niu, Sun, Zhang, Zhao, Tian, Cheng, Zheng, Guo, Zhang, Ma and Wang.)

The pathogen laboratory (p-lab) is the core and primary department of centers for disease control and prevention (CDCs) in China to respond to infectious disease outbreaks such as COVID-19. To understand the current status and capacity of p-labs in Chinese CDCs during the COVID-19 pandemic, we conducted a nationwide cross-sectional survey among 399 respondents from 239 CDCs. Differences in the current status of p-labs in CDCs of provinces, cities, and counties mainly comprised laboratory equipment, IEIs, mastery of personal occupational skills, and maximum detection capacity. Most CDCs reported a lack of staff and funds for personnel, which should be a priority in China's upcoming public health reform. The development of sequencing technologies has received considerable attention in CDCs. These are mainly used to study respiratory viruses such as influenza and SARS-CoV-2. The COVID-19 pandemic has driven development of the CDCs in China, and personnel and funds are considered key factors in improving the detection capacity of CDC p-labs., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Wang, Niu, Zhang, Li, Nie and Ma.)

In order to solve the soil problem of poor structure and low fertility after the abandoned homestead reclamation of Hollow Village in Loess Area and to improve the quality of the reclaimed soil in Hollow Village, a five-year field experiment was conducted here. In this experiment, the following seven treatments were applied: no modified material (CK), maturing agent (TM), fly ash (TF), organic fertilizer (TO), maturing agent + fly ash (TMF), maturing agent + organic fertilizer (TMO) and fly ash + organic fertilizer (TFO), and the effects of different improved materials on soil properties and crop yield were studied. The results showed that: soil organic matter (SOM) and total nitrogen contents increased significantly after the application of different improved materials, which promoted the cementation and aggregation of water-stable microaggregates (< 0.25 mm), and the water-stable macroaggregates showed an increasing trend. In the 0-0.15 m soil layer, the proportion of water-stable macroaggregates under TM, TF, TO, TMF, TMO, and TFO treatment increased by 328.2%, 130.0%, 87.8%, 81.1%, 36.7%, and 12.2% compared with CK, respectively. Meanwhile, TF, TO, TMF, TMO, TFO treatments significantly increased the mean weight diameter (MWD) and geometric mean diameter (GMD) values, reduced soil bulk density, the stable aggregate index (E LT ) and fractal dimension (D) values (P < 0.05), and the stability of soil structure and the capacity of soil moisture retention has been significantly improved. The SOM content had a significant positive correlation with MWD, GMD, and > 2 mm water-stable aggregates and a significant negative correlation with the E LT , D, and water-stable microaggregates. In particular, the organic-inorganic coupling treatment of TFO showed the highest SOM content, soil moisture content, water-stable macroaggregates and maize yield, which was the most appropriate amendment for improving the reclaimed soil structure and fertility of Hollow Village in Loess Area., (© 2022. The Author(s).)

Single nucleotide polymorphisms (SNPs) have important application value in the research of population genetics, hereditary diseases, tumors, and drug development. Conventional methods for detecting SNPs are typically based on PCR or DNA sequencing, which is time-consuming, costly, and requires complex instrumentation. In this study, we present a duplex probe-directed recombinase amplification (duplex-PDRA) assay that can perform real-time detection of two SNPs (rs6983267 and rs1447295) in four reactions in two tubes at 39°C within 30 min. The sensitivity of duplex-PDRA was 2×103-104 copies per reaction and no cross-reactivity was observed. A total of 382 clinical samples (179 prostate cancer patients and 203 controls) from northern China were collected and tested by duplex-PDRA assay and direct sequencing. The genotyping results were completely identical. In addition, the association analysis of two SNPs with prostate cancer risk and bone metastasis was conducted. We found that the TT genotype of rs6983267 (OR: 0.42; 95%CI: 0.23-0.78; P=0.005) decreased the risk of prostate cancer, while the CA genotype of rs1447295 (OR: 1.89; 95%CI: 1.20-2.96; P=0.005) increased the risk of prostate cancer. However, no association between the two SNPs (rs6983267 and rs1447295) and bone metastasis in prostate cancer was found in this study (P>0.05). In conclusion, the duplex-PDRA assay is an effective method for the simultaneous detection of two SNPs and shows great potential for widespread use in research and clinical settings.

Objective: Identifying Atrial Ventricular Hypertrophy Electrocardiogram (AVH ECG)and diagnosing the classification of theirs automatically., Methods: The ECG data used in this experiment was collected from the First Affiliated Hospital of China Medical University. CNN are combined with conventional methods and a 10 layers of one dimensional CNN are created in this experiment to extract the features of ECG signals automatically and achieve the function of classifying. ROC, sensitivity and F1-score are used here to evaluate the effects of the model., Results: In the experiment of identifying AVH ECG, the AUC of test dataset is 0.991, while in the experiment of classifying AVH ECG, the maximal F1-score can reach 0.992., Conclusions: The CNN model created in this experiment can achieve the auxiliary diagnosis of AVH ECG.

Objective: To characterise gut microbiome in patients with hepatocellular carcinoma (HCC) and evaluate the potential of microbiome as non-invasive biomarkers for HCC., Design: We collected 486 faecal samples from East China, Central China and Northwest China prospectively and finally 419 samples completed Miseq sequencing. We characterised gut microbiome, identified microbial markers and constructed HCC classifier in 75 early HCC, 40 cirrhosis and 75 healthy controls. We validated the results in 56 controls, 30 early HCC and 45 advanced HCC. We further verified diagnosis potential in 18 HCC from Xinjiang and 80 HCC from Zhengzhou., Results: Faecal microbial diversity was increased from cirrhosis to early HCC with cirrhosis. Phylum Actinobacteria was increased in early HCC versus cirrhosis. Correspondingly, 13 genera including Gemmiger and Parabacteroides were enriched in early HCC versus cirrhosis. Butyrate-producing genera were decreased, while genera producing-lipopolysaccharide were increased in early HCC versus controls. The optimal 30 microbial markers were identified through a fivefold cross-validation on a random forest model and achieved an area under the curve of 80.64% between 75 early HCC and 105 non-HCC samples. Notably, gut microbial markers validated strong diagnosis potential for early HCC and even advanced HCC. Importantly, microbial markers successfully achieved a cross-region validation of HCC from Northwest China and Central China., Conclusions: This study is the first to characterise gut microbiome in patients with HCC and to report the successful diagnosis model establishment and cross-region validation of microbial markers for HCC. Gut microbiota-targeted biomarkers represent potential non-invasive tools for early diagnosis of HCC., Competing Interests: Competing interests: None declared., (© Author(s) (or their employer(s)) 2019. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.)

Total (all forms of inorganic and organic) concentrations of mercury (Hg) and selenium (Se) were measured in dorsal muscle and eggs of wild fishes from two shallow lakes in China: Tai Lake (Ch: Taihu; TL) and Baiyangdian Lake (BYDL). Hazard quotients (HQs) were calculated by dividing concentrations of Se or Hg in muscle or eggs of fishes by threshold concentrations for effects expressed as tissue residue toxicity reference values (TR-TRVs). Concentrations of Hg in whole bodies of fishes were estimated by concentrations in muscle. Based on concentrations of Hg in whole body, HQs for fishes in TL and BYDL were less than 1.0, which suggests little to moderate potential for effects on these fishes and unaccepted adverse effects of Hg are unexpected for adult fishes. HQs of Se in muscle of common carp from TL were closed to 1.0, and 27% of HQs based on concentrations of Hg in eggs of fishes from BYDL exceeded 1.0. Potential hazard due to Hg on common carp in TL and reproductive effects of Se on fishes from BYDL exhibited need for concern. Ratios of molar concentrations of Se to Hg were greater than 1.0. Thus, there might be some protective effects of Se on effects of Hg on fishes in TL and BYDL.

PCBs are typical of persistent, bioaccumulative and toxic compounds (PBTs) that are widely distributed in the environment and can biomagnify through aquatic food webs, because of their stability and lipophilic properties. Fish-eating birds are top predators in the aquatic food chain and may suffer adverse effects from exposure to PCB concentrations. In this review, we address the toxicity of PCBs to birds and have derived tissue residue guidelines (TRGs) and toxic reference values (TRVs) for PCBs for protecting birds in China. In deriving these protective indices, we utilized available data and three approaches, to wit: species sensitivity distribution (SSD), critical study approach (CSA) and toxicity percentile rank method (TPRM). The TRGs and TRVs arrived at by using these methods were 42.3, I 0. 7, 4.3 pg TEQs/g diet wm and 16.7, 15.5, and 5.5 pg TEQs/g tissue wm for the CSA SSD and TPRM approaches, respectively. These criteria values were analyzed and compared with those derived by others. The following TRG and TRY, derived by SSD, were recommended as avian criteria for protecting avian species in China: 10.7 pg TEQs/g diet wm and 15.5 pg TEQs/g tissue wm, respectively. The hazard of PCBs to birds was assessed by comparing the TRVs and TRGs derived in this study with actual PCB concentrations detected in birds or fish. The criteria values derived in this study can be used to evaluate the risk of PCBs to birds in China, and to provide indices that are more reasonable for protecting Chinese avian species. However, several sources of uncertainty exists when deriving TRGs and TRVs for the PCBs in birds, such as lack of adequate toxicity data for birds and need to use uncertainty factors. Clearly, relevant work on PCBs and birds in China are needed in the future. For example, PCB toxicity data for resident avian species in China are needed. In addition, studies are needed on the actual PCB levels in birds and fish in China. Such information is needed to serve as a more firm foundation for future risk assessments.

Contamination of the aquatic environment by EDCs has received considerable attention from scientists, government officials, and the public. E2, one of the EDCs with high estrogenic effect, has the potential to cause multiple endocrine-disrupting effects, even at small concentrations. In the present review, the toxicity of E2 to aquatic organisms was reviewed. Results of published studies show that, for aquatic species, reproductive effects were the most sensitive endpoint for E2 exposure.Although the risks posed by EDCs have caused much attention, the research on the WQC 'for EDCs is still at the initial stage. It has been suggested in several reports that the PNEC can be regarded as the most appropriate reference value for developing WQC for the EDCs. The SSD method was applied to derive PNECs that were based on reproductive effects endpoints. In the present review, 31 NOECs, based on reproductive effect endpoints for different species, were selected to construct the curve. ThePNEC value was determined to be 0.73 ng E2/L, which could protect the biodiversity of aquatic ecosystems. Moreover, 6 NOECs for multigeneration species were also analyzed in anticipation of sensitivity comparison between the Fa and the F1 generations.When multiple generations of aquatic species were exposed to concentrations no greater than 100 ng E2/L, nearly 71.4% of the F 1 generation individuals were more sensitive to the effects of E2 than those of the Fa generation. This result indicated that different generations of the same species may respond differently to EDCs exposure.Individuals of the F 1 generation were slightly more sensitive than those of the Fa generation,in general. Therefore, protecting the F1 generation of aquatic organisms is particularly important when WQC values for the EDCs are established.Considering the toxic effects of EDCs on reproduction, long-term toxic effects(viz., full-life cycle study and the most sensitive life stage) should be used in settingWQC. Unfortunately, the NOECs of E2 for multigeneration species did not meet the requirement of PNEC derivation for protecting the Fl generation. Therefore, further research results are needed on the Fl generation of aquatic species to provide more insight into what constitutes adequate protection for aquatics lives. In the present review, the PNEC values derived in the study were compared to thePNEC values developed by others, and the results showed that they were highly consistent. In addition, we also compared the PNEC value for E2 to the PNEC value for EE2, a similar estrogen, and the result was also highly consistent when their EEFs were considered. These comparisons affirmed that the method we used for deriving the PNEC value of E2 was reasonable and the PNEC values we derived were acceptable for protecting aquatic organisms. By comparing the PNEC values we calculated to actual E2 concentrations in the natural water environment, we found that E2 in surface waters may pose high risks in many countries, especially China, Japan, the USA, Great Britain, and Italy.

MeHg is the most biologically available and toxic from of mercury, and has the potential to bioaccumulate and biomagnify as it moves up the food chain. These characteristics result in MeHg exposure to avian wildlife at high trophic levels that can produce adverse effects. The toxicity of MeHg to birds was reviewed, and using available data, TRVs and TRCs were derived for protecting birds in China. The TRV and TRC values were based on concentrations of MeHg in diet (or fish tissue based) and tissues of birds. Two methods were applied to derive TRVs from concentrations in the diet or in tissues. These were the CSA and SSD approaches. Results of published studies show that reproductive productivity of while ibis was the most sensitive endpoint for MeHg exposure, and study results on white ibises were used for deriving the TRV and TRC values, which included applying a UF of 2.0. For the SSD approach, data for ten species were used to construct the SSD for MeHg, and to calculate the dietary-based TRV and TRC values. Using the CSA approach, the TRV was based on MeHg in the diet and was derived as 5.0 ng MeHg/g (bm).day; for feathers and blood, the TRV's were 3.16 μg THg/g (wwt), and 0.365 μg THg/g (wwt), respectively. The corresponding TRCs were 15.47 ng MeHg/g (wwt), 3.16 μg THg/g (wwt)respectively. The dietary-based TRV and TRC derived by SSD were 3.09 ng MeHg (bm)/day and 9.56 ng MeHg/g (wwt) respectively. However, birds tissue residue-based criteria were not available because insufficient MeHg effects data existed to construct an SSD for birds. We compared the criteria derived in our study to those developed by others, and concluded that our results provided more reasonable protection to Chinese avian wildlife. By comparing the criteria derived values we calculated to actual MeHg levels in fish and bird tissues, we concluded that these criteria values are useful indicators for screening-level risk assessments of avian wildlife in Chinese aquatic systems. The results of this meta-analysis might therefore have important implications for assessing the risk of Hg exposure to birds and for environmental management in China and in other regions. Moreover, because humans and top avian wildlife consumers are at the same trophic level, these criteria may also be used as a reference for human health risk assessment. The diet of birds consists of aquatic species from different trophic levels. However, the structure of the food web for avian wildlife and the environmental factors that effect their exposure to MeHg vary among aquatic systems. Therefore, further research results are needed on the food web structure of avian wildlife in Chinese aquatic systems to provide more insight into what constitutes adequate protection for avian wildlife.