1. Genetic variability and functional implication of HPV16 from cervical intraepithelial neoplasia in Shanghai women.
- Author
-
Zhao J, Zhu J, Guo J, Zhu T, Zhong J, Liu M, Ruan Y, Liao S, and Li F
- Subjects
- Adult, Aged, Amino Acid Substitution, China, DNA, Viral, Female, Genotyping Techniques, Humans, Middle Aged, Mutation, Phylogeny, Protein Conformation, Squamous Intraepithelial Lesions virology, Virus Integration genetics, Young Adult, Human papillomavirus 16 genetics, Human papillomavirus 16 pathogenicity, Oncogene Proteins, Viral genetics, Papillomavirus E7 Proteins genetics, Papillomavirus Infections virology, Repressor Proteins genetics, Uterine Cervical Neoplasms virology, Uterine Cervical Dysplasia virology
- Abstract
Human papillomavirus (HPV)16 gene mutation is usually associated with persistent HPV infection and cervical intraepithelial neoplasia (CIN). However, the functional implications of HPV16 mutations remain poorly understood.145 LCR/E6/E7 of the HPV16 isolates were amplified and sequenced, and HPV16 integration status was detected. In total, 89 SNPs (68 in the LCR, 13 in E6, 8 in E7) were discovered, 11 of which were nonsynonymous mutations (8 in E6, 3 in E7). The H85Y and E120D variants in E6 were significantly reduced in the high-grade squamous intraepithelial lesion (HSIL) group compared to the
T," a potential binding site for TATA-binding protein, is the most common in LCR variants. A4 (Asian) was associated with an increased risk of HSIL compared to A1-3(P = .009). The H85/E120 in E6 and N29 in HPV16 E7 might play a critical role in carcinogenesis by disrupting p53 and Rb degradation due to affecting their interaction, respectively. In a word, the findings in this study provide preventative and therapeutic interventions of HPV16 -related cervical lesions/cancer., (© 2019 Wiley Periodicals, Inc.) - Published
- 2020
- Full Text
- View/download PDF