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Beilschmiedia mengwangensis, a new species of Beilschmiedia (Lauraceae) from Yunnan Province, Southwest China, is here described and illustrated based on morphological and phylogenetic evidence. The new species is morphologically similar and phylogenetically closely related to Beilschmiedia brachythyrsa. However, it can be distinguished from Beilschmiedia brachythyrsa by having larger fruits, longer and thicker fruiting pedicels, larger leaf blades, and more lateral veins. Taxonomic keys for identification are also provided in this study. [ABSTRACT FROM AUTHOR]

Yellowhorn (Xanthoceras sorbifolium Bunge) is an oil-bearing tree species in northern China. In this study, we used yellowhorn from Heilongjiang to analyze the morphological and physiological changes of fruit development and conducted transcriptome sequencing. The results showed that the fruit experienced relatively slow growth from fertilization to DAF20 (20 days after flowering). From DAF40 to DAF60, the fruit entered an accelerated development stage, with a rapid increase in both transverse and longitudinal diameters, and the kernel contour developed completely at DAF40. From DAF60 to DAF80, the transverse and vertical diameters of the fruit developed slowly, and the overall measures remained stable until maturity. The soluble sugar, starch, and anthocyanin content gradually accumulated until reaching a peak at DAF80 and then rapidly decreased. RNA-seq analysis revealed differentially expressed genes (DEGs) in the seed coat and kernel, implying that seed components have different metabolite accumulation mechanisms. During the stages of seed kernel development, k-means clustering separated the DEGs into eight sub-classes, indicating gene expression shifts during the fruit ripening process. In subclass 8, the fatty acid biosynthesis pathway was enriched, suggesting that this class was responsible for lipid accumulation in the kernel. WGCNA revealed ten tissue-specific modules for the 12 samples among 20 modules. We identified 54 fatty acid biosynthesis pathway genes across the genome, of which 14 was quantified and confirmed by RT-qPCR. Most genes in the plastid synthesis stage showed high expression during the DAF40-DAF60 period, while genes in the endoplasmic reticulum synthesis stage showed diverse expression patterns. EVM0012847 (KCS) and EVM0002968 (HCD) showed similar high expression in the early stages and low expression in the late stages. EVM0022385 (HCD) exhibited decreased expression from DAF40 to DAF60 and then increased from DAF60 to DAF100. EVM0000575 (KCS) was increasingly expressed from DAF40 to DAF60 and then decreased from DAF60 to DAF100. Finally, we identified transcription factors (TFs) (HB-other, bHLH and ARF) that were predicted to bind to fatty acid biosynthesis pathway genes with significant correlations. These results are conducive to promoting the transcriptional regulation of lipid metabolism and the genetic improvement in terms of high lipid content of yellowhorn. [ABSTRACT FROM AUTHOR]

'Shuijingmiyou' pummelo (SJ), one of the most popular fruits in Yunnan province of China, is of relatively low fruit shape (FS) quality. In this study, we compared the FS promoting effects of cross pollinations using pollens from seven pummelo varieties, and found that 'Guanximiyou' pummelo (GX) cross-pollination showed the best FS promoting effects on SJ fruits by shortening its fruit neck. To explore the underlying mechanism, physiochemical and transcriptomic differences between self- and cross-pollinated SJ ovaries (SJO and GXO) were investigated. Higher salicylic acid, gibberellin and indole acetic acid contents and superoxide dismutase, peroxidase and catalase activities, and lower polyphenol oxidase activity were determined in GXO compared with SJO. Enrichment analysis of the identified 578 differentially expressed genes (123 up-regulated and 455 down-regulated) in GXO showed that genes involved in solute transport, RNA biosynthesis, phytohormone action and cell wall organization were significantly enriched. The results obtained in this study will be helpful in understanding the influences of cross-pollination on pummelo ovary and fruit development, and can provide the basis for clarifying the underlying mechanism of cross-pollination improved fruit quality. [ABSTRACT FROM AUTHOR]

China's unprecedented economic growth in recent decades can largely be attributed to millions of migrant labourers that made China the 'world's factory'. It is well documented, however, that migrant labourers experience discrimination in the labour market. Despite China's phenomenal economic growth, few studies have investigated whether the working conditions of low-skilled migrants have improved. In this study, we ask: in the past two decades, have disparities in working conditions and earnings between low-skilled rural-to-urban migrant workers and their urban counterparts decreased? We contrast the working conditions and earnings between migrants and urban residents who lack a high school degree and age 18-55 years using panel data, the China Health and Nutrition Survey (1993-2015), and individual fixed effects models to account for selection problems. Findings indicate that over a span of twenty years migrants and urban residents had similar trends in employment and earned incomes, but migrants' weekly working hours and tendency to work more than legal work hours increased more than urban residents'. Working longer without earning more suggests persistent social inequality in Chinese society in general and deeper injustice among migrant workers. [ABSTRACT FROM AUTHOR]

The Chinese plum (Prunus salicina L.) is a fruit tree belonging to the Rosaceae family, native to south-eastern China and widely cultivated throughout the world. Fruit sugar metabolism and color change is an important physiological behavior that directly determines flavor and aroma. Our study analyzed six stages of fruit growth and development using RNA-seq, yielding a total of 14,973 DEGs, and further evaluation of key DEGs revealed a focus on sugar metabolism, flavonoid biosynthesis, carotenoid biosynthesis, and photosynthesis. Using GO and KEGG to enrich differential genes in the pathway, we selected 107 differential genes and obtained 49 significant differential genes related to glucose metabolism. The results of the correlation analyses indicated that two genes of the SWEET family, evm.TU.Chr1.3663 (PsSWEET9) and evm.TU.Chr4.676 (PsSWEET2), could be closely related to the composition of soluble sugars, which was also confirmed in the ethylene treatment experiments. In addition, analysis of the TOP 20 pathways between different growth stages and the green stage, as well as transient overexpression in chili, suggested that capsanthin/capsorubin synthase (PsCCS) of the carotenoid biosynthetic pathway contributed to the color change of plum fruit. These findings provide an insight into the molecular mechanisms involved in the ripening and color change of plum fruit. [ABSTRACT FROM AUTHOR]

The olecranon honey peach is China's national geographic identification product because of its crisp texture and high sweetness. In recent years, new field management practices have been developed. In this study, fruits ('SJH') grown through new planting methods, i.e., black bags combined with bio-organic fermentation fertilizer, were compared with those ('SBY') grown by conventional planting methods, i.e., using a chemical fertilizer without bagging, to determine their effects on improving fruit quality. At maturity, the 'SJH' was significantly higher than 'SBY' in terms of weight, hardness, and sugar content by 14.43%, 19.55% and 9.66%, respectively. RNA sequencing (RNA-seq) technology analysis was performed on 'SJH' and 'SBY' to identify the main regulatory pathways involved in fruit development, especially focusing on cell-wall biogenesis and sugar metabolism. We identified a total of 1660 differentially expressed genes (DEGs) in 'SJH' and 5673 genes in 'SBY' throughout the development. A clustering analysis of DEGs revealed that the expression patterns of key genes involved in cell-wall biogenesis and sugar metabolism pathways in 'SJH' differed from those in 'SBY', such as cellulose synthase-like protein D (CS-LPD), sucrose-phosphate synthase (SPS) and sucrose synthase (SUS). The new cultivation technology promoted soluble sugar accumulation and cell-wall synthesis through molecular regulation, which improved the sweetness and sensory quality of the fruit. These findings contribute towards to the development of novel ideas for the better cultivation of peaches and provide a deeper investigation into the molecular mechanism of their development. [ABSTRACT FROM AUTHOR]

Wolfberry (Lycium barbarum) is a traditional cash crop in China and is well-known worldwide for its outstanding nutritional and medicinal value. Lycium ruthenicum is a close relative of Lycium barbarum but differs significantly in size, color, flavor and nutritional composition. To date, the metabolic differences between the fruits of the two wolfberry varieties and the genetic basis behind them are unclear. Here, we compared metabolome and transcriptome data of two kinds of wolfberry fruits at five stages of development. Metabolome results show that amino acids, vitamins and flavonoids had the same accumulation pattern in various developmental stages of fruit but that Lycium ruthenicum accumulated more metabolites than Lycium barbarum during the same developmental stage, including L-glutamate, L-proline, L-serine, abscisic acid (ABA), sucrose, thiamine, naringenin and quercetin. Based on the metabolite and gene networks, many key genes that may be involved in the flavonoid synthesis pathway in wolfberry were identified, including PAL, C4H, 4CL, CHS, CHI, F3H, F3'H and FLS. The expression of these genes was significantly higher in Lycium ruthenicum than in Lycium barbarum, indicating that the difference in the expression of these genes was the main reason for the variation in flavonoid accumulation between Lycium barbarum and Lycium ruthenicum. Taken together, our results reveal the genetic basis of the difference in metabolomics between Lycium barbarum and Lycium ruthenicum and provide new insights into the flavonoid synthesis of wolfberry. [ABSTRACT FROM AUTHOR]

In China, the wild population of Nanjing Linden (Tilia miqueliana M.) is experiencing a drastic decline, primarily due to high levels of empty seeds. This study aimed to measure the biochemical and physiological changes during fruit and seed development in T. miqueliana to determine the developmental mechanism. The weight method and photosynthetic respiration were used to determine the biological aspects of both fruits and embryos, while transmission electron microscopy and the anthrone colorimetric method were used to determine the endosperm content, including sugar, starch, protein, and fat. Enzyme-linked immunosorbent assays were conducted to determine the levels of endogenous plant hormones such as indole-3-acetic acid (IAA), gibberellic acid 3 (GA3), zeatin riboside (ZR), and abscisic acid (ABA). The nonlinear least-squares method was used to fit the model of nutrient and hormone levels, revealing that fruit size expanded from the 5th to the 65th day and that fruit moisture content exhibited a downward trend, along with a decrease in fruit respiration intensity. Embryos were found to be fully developed between 35 DAF and 65 DAF, while the nutrients in the endosperm, i.e., sugar, starch, protein, and fat, continuously accumulated after 50 DAF. Additionally, ABA, IAA, GA3, and ZR contents were found to synergistically regulate seed development and maturation. [ABSTRACT FROM AUTHOR]

Due to its unique flavor and high antioxidant content, the sea buckthorn (genus Hippophae L.) fruit is increasingly favored by consumers. Developing from the perianth tube, the sea buckthorn fruit varies greatly among species in both size and shape. However, the cellular regulation mechanism of sea buckthorn fruit morphogenesis remains unclear. This study presents the growth and development patterns, morphological changes, and cytological observations of the fruits of three Hippophae species (H. rhamnoides ssp. sinensis, H. neurocarpa, and H. goniocarpa). The fruits were monitored every 10–30 days after anthesis (DAA) for six periods in their natural population on the eastern margin of the Qinghai-Tibet Plateau in China. The results showed that the fruits of H. rhamnoides ssp. sinensis and H. goniocarpa grew in a sigmoid mode, while H. neurocarpa grew in an exponential mode under the complex regulation of cell division and cell expansion. In addition, cellular observations showed that the mesocarp cells of H. rhamnoides ssp. sinensis and H. goniocarpa were larger in the area with prolonged cell expansion activity, while H. neurocarpa had a higher cell division rate. Elongation and proliferation of the mesocarp cells were found to be essential factors affecting the formation of fruit morphology. Finally, we established a preliminary cellular scenario for fruit morphogenesis in the three species of sea buckthorn. Fruit development could be divided into a cell division phase and a cell expansion phase with an overlap between 10 and 30 DAA. In particular, the two phases in H. neurocarpa showed an additional overlap between 40 and 80 DAA. The description of the sea buckthorn fruit's transformation and its temporal order may provide a theoretical basis to explore the growth mechanism of fruits and regulate their size through certain cultivation techniques. [ABSTRACT FROM AUTHOR]

Hawthorn (Crataegus pinnatifida var. major), a cultivated fruit tree, is native and unique to China. Its fruits have high nutritional, health, and medicinal values. However, the development and ripening process of hawthorns is accompanied by dramatic changes in flavor, aroma, and bioactive phytochemicals, which are the fundamental factors that contribute to the potential health benefits and establishment of fruit quality. Therefore, an exploration of the dynamic changes in metabolites and their regulatory networks during the development of hawthorn fruits can elucidate the formation mechanisms of active substances in hawthorn fruits. In this study, we used a broad targeted metabolomics approach to identify and analyze the dynamics of metabolites in hawthorn fruits at five developmental stages. The results revealed 998 primary and secondary metabolites that were classified into 15 categories. The accumulation levels of most sugars increased during fruit development and then accelerated at the fruit ripening stage. The accumulation levels of a few organic acids (e.g., citric acid, isocitric acid, and quinic acid) continuously increased. Many organic acids exhibited significant decreasing trends. Among the 561 secondary metabolites detected, 189 were phenolic acids and 199 were flavonoids. The levels of many flavonoids were significantly reduced at later stages of fruit development; in contrast, the levels of two anthocyanins significantly increased during fruit ripening. Correlation analysis revealed that there is a certain correlation within and between primary as well as secondary metabolites during fruit development. Furthermore, the integration of metabolomic and transcriptomic data in this study revealed that changes in the expression of some differentially expressed genes (DEGs) were associated with the accumulation of metabolites such as sugars, organic acids, and flavonoids, e.g., the upregulated expression levels of CS (citrate synthase) genes were consistent with the continued accumulation of citric acid. Overall, this study demonstrates the metabolic changes that occur during the development of hawthorn fruit, explores the molecular mechanisms that underlie metabolite changes during fruit development, and lays a strong theoretical foundation for the improvement of hawthorn fruit quality and the development of functional components. [ABSTRACT FROM AUTHOR]

Copyright of Acta Agriculturae Zhejiangensis is the property of Acta Agriculturae Zhejiangensis Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Lycium barbarum Thunb. and Lycium ruthenicum Murray (wolfberries) have been utilized as traditional medicinal and nutritional plants in China for centuries. Much research has been focused on their high quality, yet the molecular mechanisms underlying morphological differences remain unclear. In this study, a comparative analysis of morphological and cytological characteristics indicated that significant differences existed. Meanwhile, transcriptomic analyses of the flower and fruit were performed at different developmental stages, and a total of 54 795 differentially expressed genes (DEGs) were screened. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses showed that these DEGs were significantly enriched in substance metabolism, catalytic activity, single organism process, starch and sucrose metabolism, carotenoid biosynthesis, amino sugar and nucleotide sugar metabolism, phenylpropanoid biosynthesis, and other pathways. Based on these significantly enriched pathways, the ratio between nonsynonymous and synonymous substitution rates (Ka/Ks), and numerous studies related to flower and fruit development, we preliminarily screened eight transcription factor families related to flower and fruit development and counted the number of potential transcription factor genes. These candidate genes could provide a basis for future functional verification, helping to further research on the molecular mechanism of morphological differences in the two Lycium species. [ABSTRACT FROM AUTHOR]

Stauntonia obovatifoliola Hayata subsp. urophylla is a novel edible and healthy fruit in China, commonly known as "Jiuyuehuang" (September yellow). The fully ripe fruit of S. obovatifoliola subsp. urophylla has a soft fruit pulp texture, golden flesh, and sweet flavor which is very popular with the locals. In this paper, we have investigated the fruit appearance quality, physiochemical quality, and nutritional quality of S. obovatifoliola subsp. urophylla that was harvested at six stages (S1: 60 DAFB, S2: 90 DAFB, S3: 130 DAFB, S4: 160 DAFB, S5: 190 DAFB, S6: 205 DAFB). An increase in fruit size (including single fruit weight, fruit length, and fruit diameter) was related to the ripeness stage of fruit development. The total soluble solids, firmness, dry matter, sugar and starch showed remarkable changes as the fruit approached ripening (S5–S6 stage). The main sugar components in the fruit were fructose, glucose, and maltose. The contents of fructose, glucose, and total sugars in S. obovatifoliola subsp. urophylla fruit progressively increased from the S1 to the S6 stage while increasing sharply from the S4 to the S5 stage. As for the content of maltose and starch, they both showed an increasing trend from the S1 to the S4 stage but decreased sharply at the S5 stage. The vitamin B, vitamin C, total phenolics, total flavonoids, and amino acid levels showed an overall downward trend during fruit development. To our knowledge, this is the first study to compare the phytochemical characteristics, nutrient composition, and antioxidant content during the different fruit development stages. The results of this study may provide a scientific basis for clarifying the growth and development characteristics of S. obovatifoliola subsp. urophylla fruit and the further utilization of these excellent medicinal and edible germplasm resources. [ABSTRACT FROM AUTHOR]

In order to explore the effect of malic acid accumulation, spraying the foliar calcium and magnesium of ‘Feizixiao' litchi, during the period of ‘Feizixiao' litchi fruit development, 0.3% magnesium chloride(Mg), 0.3% calcium chloride(Ca), their mixture (Ca+Mg) and clean water(CK) were sprayed on leaves, and contents of water-soluble calcium, water-soluble magnesium and malic acid and the activities of malic acid metabolism-related enzymes in fruit flesh were measured. The multivariate linear correlation analysis were also performed. The results were as follows: (1) The content of malic acid showed a L-shaped trend, Mg, Ca and Ca+Mg treatments might promote the accumulation of malic acid in the early stage of fruit growth and development, while Ca treatment might promote the accumulation of malic acid in the late stage. (2)The content of water-soluble calcium in the flesh showed increasing trend, and the content of water-soluble magnesium showed the trend like “M”.(3)The content of malic acid was positively correlated with the activities of NADP-ME in CK and Ca, and PEPC and NAD-MDH in Ca+Mg, while the content of malic acid was negatively correlated with the activities of PEPC and MS in CK. (4)The water-soluble calcium inhibited the activities of NAD-MDH and NADP-ME, while water-soluble magnesium inhibited the activities of NADP-MDH and MS. In conclusion, foliar spraying of calcium and magnesium fertilizers can affect the contents of water-soluble calcium, magnesium and malic acid metabolism-related enzymes, and the linear correlation also changes. Different treatments can affect the accumulation of malic acid in pulp and cause the change of total acid content in pulp. Ca treatment may inhibit pulp respiration by accumulation of malic acid and then reducing the loss of sugar in pulp, which can be used as fertilizer technology in production. This results provide the theoretical reference and technical support for the actual production of litchi in China. [ABSTRACT FROM AUTHOR]

• Determining GAs contents in lychee improves our understanding of its physiological mechanisms of fruit and flower development. • A straightforward pre-treatment procedure was optimized for the extraction of lychee GAs. • A high-efficiency UHPLC-QQQ-MS/MS method was established for simultaneous quantitation of bioactive GAs in diverse lychee samples. • GA 1 and GA 3 may be important for lychee fruit development, while GA 4 and GA 7 may be vital for the flower development. Gibberellins (GAs) are a group of phytohormones that have profound and diverse effects on plant growth and development. Within this group, bioactive GAs, including GA1, GA3, GA4, and GA7, play a particularly significant role in regulating these processes. Lychee (Litchi chinensis Sonn.) is a tropical fruit tree native to southern China. Optimization of rapid and precise methods for analyzing bioactive GAs in lychee is crucial to enhance our understanding of the underlying physiological mechanisms of fruit and flower development and to explore its potential industrial applications in this fruit tree. In this study, we developed a high-efficiency method for simultaneous quantitation of bioactive GAs in lychee using ultra-high performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry (UHPLC-QQQ-MS/MS). Ultimately, this method was effectively utilized to quantify the bioactive GAs in various tissues of lychee, including fruits, seeds, and flowers. The developed method exhibited satisfactory recoveries and precision, as well as short chromatographic run time and low limits of detection and quantitation of bioactive GAs in lychee. This protocol provides a valuable method for the characterization of physiological roles and molecular mechanism of GAs in lychee. [ABSTRACT FROM AUTHOR]

PA-enhanced content causes astringency in persimmon fruit. PCNA persimmons can lose their astringency naturally and they become edible when still on the tree, which allows for conserves of physical and financial resources. C-PCNA persimmon originates in China. Its deastringency trait primarily depends on decreased PA biosynthesis and PA insolubilization at the late stage of fruit development. Although some genes and transcription factors that may be involved in the deastringency of C-PCNA persimmon have been reported, the expression patterns of these genes during the key deastringency stage are reported less. To investigate the variation in PA contents and the expression patterns of deastringency-related genes during typical C-PCNA persimmon 'Xiaoguo-tianshi' fruit development and ripening, PA content and transcriptional profiling were carried out at five late stages from 70 to 160 DAF. The combinational analysis phenotype, PA content, and DEG enrichment revealed that 120–140 DAF and 140–160 DAF were the critical phases for PA biosynthesis reduction and PA insolubilization, respectively. The expression of PA biosynthesis-associated genes indicated that the downregulation of the ANR gene at 140–160 DAF may be associated with PA biosynthesis and is decreased by inhibiting its precursor cis-flavan-3-ols. We also found that a decrease in acetaldehyde metabolism-associated ALDH genes and an increase in ADH and PDC genes might result in C-PCNA persimmon PA insolubilization. In addition, a few MYB-bHLH-WD40 (MBW) homologous transcription factors in persimmon might play important roles in persimmon PA accumulation. Furthermore, combined coexpression network analysis and phylogenetic analysis of MBW suggested that three putative transcription factors WD40 (evm.TU.contig1.155), MYB (evm.TU.contig8910.486) and bHLH (evm.TU.contig1398.203), might connect and co-regulate both PA biosynthesis and its insolubilization in C-PCNA persimmon. The present study elucidated transcriptional insights into PA biosynthesis and insolubilization during the late development stages based on the C-PCNA D. kaki genome (unpublished). Thus, we focused on PA content variation and the expression patterns of genes involved in PA biosynthesis and insolubilization. Our work has provided additional evidence on previous knowledge and a basis for further exploration of the natural deastringency of C-PCNA persimmon. [ABSTRACT FROM AUTHOR]

Vaccinium bracteatum Thunb. (VBT) is widely distributed in the mountainous areas in eastern and southern China. VBT leaves have great medical value and can be used to stain rice to produce "Wumifan". Its fruits also contain rich nutrients. However, there has been limited attention to exploring the molecular content of VBT. Previously, we performed RNA-seq on three typical VBT fruits that were at various stages of ripening, although a reliable reference gene was lost in validation.In this study, we selected ten candidate reference genes based on previous studies and transcriptomics analyses. Subsequently, these genes were evaluated using a combination of methods, including geNorm, NormFinder, and Bestkeeper, with a comprehensive ranking assessment. As a result, we found that the actin2, NADH, and ADK genes have high reliability for analysing the expression levels of genes involved in fruit development. Furthermore, the transcript levels of 15 DEGs from transcriptomic analysis were assessed using NADH as a reference gene, and RT-qPCR data were highly consistent with the transcriptomic data. These results provide reliable reference genes for further studying gene expression, which will be beneficial for comprehensively exploring VBT. [ABSTRACT FROM AUTHOR]

Simple Summary: Pear is a widely eaten fruit all over the world. Volatile aroma is an important factor affecting fruit quality and the fatty acid metabolism pathway is important in synthesizing volatile aromas. In this study, Panguxiang (Pyrus spp. Panguxiang) is a new variety bred from P. bretschneideri Rehd. cv. 'Biyang piaoli' and, unlike most white pear varieties cultivated in China, its aroma is also vital. The study aimed to explore unique pear resources of rich fruit aroma and to clarify the metabolism and regulation mechanism of the aromatic components in pear fruit. This paper used physiological and transcriptome methods to explore the molecular network behind volatile aroma formation in Panguxiang revealed via fatty acid metabolic pathways. Through transcriptome sequencing, weighted gene co-expression network analysis (WGCNA) identified yellow functional modules and several biological and metabolic pathways related to fatty acid formation. Finally, we identified seven and eight hub genes in the fatty acid synthesis and fatty acid metabolism pathways, respectively. Further analysis of the co-expression network allowed us to identify several key transcription factors related to the volatile aroma, including AP2/ERF-ERF, C3H, MYB, NAC, C2H2, GRAS, and Trihelix, which may also be involved in fatty acid synthesis and further influence the formation of aroma. Pears are popular table fruits, grown and consumed worldwide for their excellent color, aroma, and taste. Volatile aroma is an important factor affecting fruit quality, and the fatty acid metabolism pathway is important in synthesizing volatile aromas. Most of the white pear varieties cultivated in China are not strongly scented, which significantly affects their overall quality. Panguxiang is a white pear cultivar, but its aroma has unique components and is strong. The study of the mechanisms by which aroma is formed in Panguxiang is, therefore, essential to improving the quality of the fruit. The study analyzed physiological and transcriptome factors to reveal the molecular network behind volatile aroma formation in Panguxiang. The samples of Panguxiang fruit were collected in two (fruit development at 60, 90, 120, and 147 days, and fruit storage at 0, 7, 14, 21, and 28 days) periods. A total of nine sample stages were used for RNA extraction and paired-end sequencing. In addition, RNA quantification and qualification, library preparation and sequencing, data analysis and gene annotation, gene co-expression network analysis, and validation of DEGs through quantitative real-time PCR (qRT-;PCR) were performed in this study. The WGCNA identified yellow functional modules and several biological and metabolic pathways related to fatty acid formation. Finally, we identified seven and eight hub genes in the fatty acid synthesis and fatty acid metabolism pathways, respectively. Further analysis of the co-expression network allowed us to identify several key transcription factors related to the volatile aroma, including AP2/ERF-ERF, C3H, MYB, NAC, C2H2, GRAS, and Trihelix, which may also be involved in the fatty acid synthesis. This study lays a theoretical foundation for studying volatile compounds in pear fruits and provides a theoretical basis for related research in other fruits. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Oil Crop Sciences is the property of Oil Crops Research Institute of Chinese Academy of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Camellia oleifera is an economic tree species in southern China and is famous for its oil. The surrounding climate is filtered by the tree itself, resulting in the canopy microclimate, which affects the growth and fruit quality of C. oleifera. This study investigated the effect of canopy positions on microclimate and fruit growth, maturation and qualities by comparing the differences in canopy position. This study also considered the relationship between microclimate and fruit qualities during the oil conversion period. The fruit qualities and microclimate were studied by dividing the canopy into two vertical layers and horizontal layers, creating the following canopy positions: upper outer canopy (UO), upper inner canopy (UI), lower outer canopy (LO) and lower inner canopy (LI). The light intensity increased significantly from inside to outside and from top to bottom in the canopy; however, there were no significant differences in temperature and relative humidity. At maturity, the moisture content of fruits and kernels in UO and LO was approximately <5% of those in UI and LI. The soluble sugar content increased by 10.90%, 8.47% and 6.84% in UO, UI and LO in November, while no significant change was observed in LI. The kernel oil content (KOC) obtained a higher value in UO and UI at maturity. However, KOC decreased by 5.16%, 3.02%, 3.10% and 0.67% in UO, UI, LO and LI in November. Light intensity in September and October was correlated, and temperature and relative humidity in August and September were correlated. [ABSTRACT FROM AUTHOR]

Chinese jujube (Ziziphus jujuba) is a vital economic tree native to China. Jujube fruit with abundant L-Ascorbic Acid (AsA) is an ideal material for studying the mechanism of AsA biosynthesis and metabolism. However, the key transcription factors regulating AsA anabolism in jujube have not been reported. Here, we used jujube variety "Mazao" as the experimental material, conducted an integrative analysis of transcriptome and metabolome to investigate changes in differential genes and metabolites, and find the key genes regulating AsA during jujube fruit growth. The results showed that AsA was mostly synthesized in the young stage and enlargement stage, ZjMDHAR gene takes an important part in the AsA recycling. Three gene networks/modules were highly correlated with AsA, among them, three genes were identified as candidates controlling AsA, including ZjERF17 (LOC107404975), ZjbZIP9 (LOC107406320), and ZjGBF4 (LOC107421670). These results provide new directions and insights for further study on the regulation mechanism of AsA in jujube. [ABSTRACT FROM AUTHOR]

Cucumbers are rich in vitamins and minerals. The cucumber has recently become one of China's main vegetable crops. More specifically, the adjustment of the Chinese agricultural industry's structure and rapid economic development have resulted in increases in the planting area allocated to Chinese cucumber varieties and in the number of Chinese cucumber varieties. After complete sequencing of the "Chinese long" genome, the transcriptome, proteome, and metabolome were obtained. Cucumber has a small genome and short growing cycle, and these traits are conducive to the application of molecular breeding techniques for improving fruit quality. Here, we review the developments and applications of molecular markers and genetic maps for cucumber breeding and introduce the functions of gene families from the perspective of genomics, including fruit development and quality, hormone response, resistance to abiotic stress, epitomizing the development of other omics, and relationships among functions. [ABSTRACT FROM AUTHOR]

Fruit setting and ripening are crucial in the reproductive cycle of many desert plant species, but their response to precipitation changes is still unclear. To clarify the response patterns, a long-term in situ water addition experiment with five treatments, namely natural precipitation (control) plus an extra 25%, 50%, 75%, and 100% of the local mean annual precipitation (145 mm), was conducted in a temperate desert in northwestern China. A whole series of fruiting events including the onset, peak, and end of fruit setting and the onset, peak, and end of fruit ripening of a locally dominant shrub, Nitraria tangutorum, were observed from 2012 to 2018. The results show that (1) water addition treatments had no significant effects on all six fruiting events in almost all years, and the occurrence time of almost all fruiting events remained relatively stable compared with leaf phenology and flowering phenology after the water addition treatments; (2) the occurrence times of all fruiting events were not correlated to the amounts of water added in the treatments; (3) there are significant inter-annual variations in each fruiting event. However, neither temperature nor precipitation play key roles, but the preceding flowering events drive their inter-annual variation. [ABSTRACT FROM AUTHOR]

To determine the heat unit requirement and assess its subsequent effects on flowering and fruiting characteristics, a field experiment was conducted during 2018-19 with seven cultivars of litchi viz., Calcuttia, Elaichi, Bedana, Bombai, China, Shahi and Muzaffarpur in randomized block design. Bedana showed better result in terms of maximum fruit weight (17.88g), lowest seed content (10.84%), maximum fruit diameter (3.01 cm), maximum fruit volume (18.70 ml), highest TSS (15.870 Brix), total sugar (15.96%), reducing sugar (12.61%), and ascorbic acid (29.47 mg/100g) content. [ABSTRACT FROM AUTHOR]

As widely planted fruits with high nutritional and medical values, pomelos are managed systematically to achieve the largest economic benefits. But the annual shedding of young pomelos, which could be applied as feedstocks for essential oil extraction with their abundant volatiles, leads to a waste of source. The present study selected two commonly planted pomelo (Citrus maxima (Burm.) Merr.) varieties in Southern China, to investigate the volatile profiles during young pomelo fruits development. Combing transcriptomic analysis, this study aimed at identifying the prominent volatile components in young pomelo fruits in order to preferably extract profitable volatiles, as well, increasing the knowledge concerning regulatory roles of transcription factors (TFs) on volatiles accumulation in young pomelos. Totally 29 volatiles were identified, including 14 monoterpenoids and 13 sesquiterpenoids. Diprene was the principal component with the highest amount. Volatiles were generally decreased during fruits development but preferable stages were figured out for volatile collections. 12 and 17 TFs were related to developing time while ERF003 and MYC2 were highly correlated to monoterpenoids. These findings put forward the comprehensive usages of young pomelos and enriched the regulatory roles of TFs on both fruit development and volatiles metabolism. [ABSTRACT FROM AUTHOR]

Defoliation is used to ease mechanical harvest and reduce lint staining of cotton (Gossypium hirsutum L.). Crop conditions are important determinants of its efficiency, but their effects have not been fully examined. We performed a multiple‐site field study during 2018–2020 (under 17 environmental conditions, covering three cotton regions in China) to explore the relationship between boll retention and defoliation at the fruiting site level. The hormonal defoliant, thidiazuron and boll opener, ethephon, were tank‐mixed or their combination product was used in this study. There was a positive Spearman ranking correlation between boll retention and defoliation in most experimental sites, with correlation coefficients (r) ranging from.275 to.725 at 7 d after application of harvest aids, suggesting that the leaves at fruiting sites bearing bolls dropped more easily than those at fruiting sites where abscission occurred. The positive correlation declined with the fruiting sites up the plant and away the mainstem. The differences in defoliation between fruiting sites with or without bolls were high (around 60–70%) at lower and/or inner positions, but low or negligible at upper and/or outer positions. It appears that the higher light intensity at upper and outer portions of plant may enhance leaf drop and thus reduce the dependence of defoliation on boll retention. In conclusion, a high boll retention would accelerate leaf removal, and may enhance leaf abscission on cloudy days or within more closed canopy. Core Ideas: There was a significant positive correlation between cotton boll retention and defoliation.The positive correlation weakened over time.The positive correlation is closer at lower and first fruiting nodes than other position. [ABSTRACT FROM AUTHOR]

Background: Hami melon (Cucumis melo var. saccharinus) is a popular fruit in China because of its excellent taste, which is largely determined by its physicochemical characteristics, including flesh texture, sugar content, aroma, and nutrient composition. However, the mechanisms by which these characteristics are regulated have not yet been determined. In this study, we monitored changes in the fruits of two germplasms that differed in physicochemical characteristics throughout the fruit development period. Results: Ripe fruit of the bred variety 'Guimi' had significantly higher soluble sugar contents than the fruit of the common variety 'Yaolong.' Additionally, differences in fruit shape and color between these two germplasms were observed during development. Comparative transcriptome analysis, conducted to identify regulators and pathways underlying the observed differences at corresponding stages of development, revealed a higher number of differentially expressed genes (DEGs) in Guimi than in Yaolong. Moreover, most DEGs detected during early fruit development in Guimi were associated with cell wall biogenesis. Temporal analysis of the identified DEGs revealed similar trends in the enrichment of downregulated genes in both germplasms, although there were differences in the enrichment trends of upregulated genes. Further analyses revealed trends in differential changes in multiple genes involved in cell wall biogenesis and sugar metabolism during fruit ripening. Conclusions: We identified several genes associated with the ripening of Hami melons, which will provide novel insights into the molecular mechanisms underlying the development of fruit characteristics in these melons. [ABSTRACT FROM AUTHOR]

Red bayberry is a sweet, tart fruit native to China and grown widely in the south. The key organic compounds forming the distinctive aroma in red bayberry, are terpenoids, mainly β-caryophyllene and α-pinene. However, the key genes responsible for different terpenoids are still unknown. Here, transcriptome analysis on samples from four cultivars, during fruit development, with different terpenoid production, provided candidate genes for volatile organic compound (VOC) production. Terpene synthases (TPS) are key enzymes regulating terpenoid biosynthesis, and 34 TPS family members were identified in the red bayberry genome. MrTPS3 in chromosome 2 and MrTPS20 in chromosome 7 were identified as key genes regulating β-caryophyllene and α-pinene synthesis, respectively, by qRT-PCR. Subcellular localization and enzyme activity assay showed that MrTPS3 was responsible for β-caryophyllene (sesquiterpenes) production and MrTPS20 for α-pinene (monoterpenes). Notably, one amino acid substitution between dark color cultivars and light color cultivars resulted in the loss of function of MrTPS3, causing the different β-caryophyllene production. Our results lay the foundation to study volatile organic compounds (VOCs) in red bayberry and provide potential genes for molecular breeding. [ABSTRACT FROM AUTHOR]

Euscaphis konishii is an evergreen plant that is widely planted as an industrial crop in Southern China. It produces red fruits with abundant secondary metabolites, giving E. konishii high medicinal and ornamental value. Auxin signaling mediated by members of the AUXIN RESPONSE FACTOR (ARF) and auxin/indole-3-acetic acid (Aux/IAA) protein families plays important roles during plant growth and development. Aux/IAA and ARF genes have been described in many plants but have not yet been described in E. konishii. In this study, we identified 34 EkIAA and 29 EkARF proteins encoded by the E. konishii genome through database searching using HMMER. We also performed a bioinformatic characterization of EkIAA and EkARF genes, including their phylogenetic relationships, gene structures, chromosomal distribution, and cis -element analysis, as well as conserved motifs in the proteins. Our results suggest that EkIAA and EkARF genes have been relatively conserved over evolutionary history. Furthermore, we conducted expression and co-expression analyses of EkIAA and EkARF genes in leaves, branches, and fruits, which identified a subset of seven EkARF genes as potential regulators of triterpenoids and anthocyanin biosynthesis. RT-qPCR, yeast one-hybrid, and transient expression analyses showed that EkARF5.1 can directly interact with auxin response elements and regulate downstream gene expression. Our results may pave the way to elucidating the function of EkIAA and EkARF gene families in E. konishii , laying a foundation for further research on high-yielding industrial products and E. konishii breeding. [ABSTRACT FROM AUTHOR]

[Objective] The present paper aimed to explore the effects of different types of fruit bags on the formation of fruit quality and the change of peel pigment of Qiumitao No. 1, so as to provide reference for the high-quality production of Qiumitao No. 1 in southern China. [Method] Taking the fruit of Qiumitao No. 1 as the experimental material, four different types of fruit bags (yellow outside and black inside, single yellow bag, single white bag, single semi white translucent bag) were bagged after the first physiological fruit drop. The changes of pigment content in single fruit weight, vertical and horizontal diameters, fruit size, peel pigment, and pulp glyc acid content and relative enzyme activities were measured and analyzed every 7 days (5 times), before plucking fruit, and the change rule was analyzed. [Result] The results showed that the surface of fruit covered with yellow outside and black inside double-layer bag was smooth, the chlorophyll content was significantly lower than that of single-layer bag and showing white jade color (P < 0. OS, the same as below), the longitudinal diameter, transverse diameter and single fruit weight were higher than those of the other three treatments, and the content of quinic acid, malic acid, sucrose, sorbitol and total sugar of fruit covered with yellow outside and black inside double-layer bag were significantly lower than those of other single-layer bag treatments, which match with the light taste at maturity. Among the three single-layer bags, the yellow singlelayer bag had higher sucrose and fructose content, lower citric acid and quinic acid content, the highest total sugar content, the lowest total acid content and the highest sugar acid ratio. The results of correlation analysis showed that there was no significant correlation between the acid invertase activity and neutral invertase activity with sucrose content at late stage of fruit development (P > 0. OS). [Conclusion] Qiumitao No. 1 bagged with double-layer yellow outside and black inside has low pigment content and beautiful appearance, but the content of sugar and acid was lower, and the taste was lighter; the peel pigment content of Qiumitao No. 1 bagged with single-layer yellow fruit bag was higher than that of double-layer bag, and the fruit appearance was green and dull, but the content of sugar and acid in the fruit was higher than that of other bagged fruits. In production and markting, the type of fruit bag can be selected according to the needs of sales, which can improve the overall economic benefits. [ABSTRACT FROM AUTHOR]

Background: The oil-tea tree (Camellia oleifera Abel.) is a woody tree species that produces edible oil in the seed. C. oleifera oil has high nutritional value and is also an important raw material for medicine and cosmetics. In China, due to the uncertainty on maturity period and oil synthesis mechanism of many C. oleifera cultivars, growers may harvest fruits prematurely, which could not maximize fruit and oil yields. In this study, our objective was to explore the mechanism and differences of oil synthesis between two Camellia oleifera cultivars for a precise definition of the fruit ripening period and the selection of appropriate cultivars. Results: The results showed that 'Huashuo' had smaller fruits and seeds, lower dry seed weight and lower expression levels of fatty acid biosynthesis genes in July. We could not detect the presence of oil and oil bodies in 'Huashuo' seeds until August, and oil and oil bodies were detected in 'Huajin' seeds in July. Moreover, 'Huashuo' seeds were not completely blackened in October with up to 60.38% of water and approximately 37.98% of oil in seed kernels whose oil content was much lower than normal mature seed kernels. The oil bodies in seed endosperm cells of 'Huajin' were always higher than those of 'Huashuo' from July to October. Conclusion: Our results confirmed that C. oleifera 'Huashuo' fruits matured at a lower rate compared to 'Huajin' fruits and that 'Huajin' seeds entered the oil synthesis period earlier than 'Huashuo' seeds. Moreover, 'Huashuo' fruits did not mature during the Frost's Descent period (October 23–24 each year). [ABSTRACT FROM AUTHOR]

Commercial cultivars of kiwifruit (Actinidia spp.) can have green-fleshed or yellow-fleshed fruit depending on their chlorophyll degradation ability. In recent years, an increasing number of yellow-fleshed cultivars have been bred in China. However, the mechanisms underlying the flesh colouration of these new cultivars remain elusive. In this study, the pigment contents of the yellow-fleshed cultivar 'Jinshi NO.1' were determined during fruit development, and RNA sequencing was conducted to screen for differentially expressed genes and identify potential candidates for pigment metabolism. The chlorophyll content continuously decreased from 0 days after full bloom (DAFB) (T1) to 189 DAFB (T4), resulting in a more than 30-fold change, while the contents of anthocyanins and carotenoids showed sharp decreases from T1 to 44 DAFB (T2) and remained stable thereafter. Weighted gene co-expression network analysis (WGCNA) indicated that the chlorophyll contents were highly correlated with chlorophyll-degradation-related genes, especially three chlorophyll a/b-binding protein (CAB) genes and one stay-green gene (AcSGR1), but no carotenoid-related genes. Furthermore, these genes were found to be highly correlated with 15 transcription factors and 11 hub genes. These results indicate the important role of chlorophyll degradation in the colouration of 'Jinshi NO.1' and provide candidates for further functional characterisation and transcriptional regulation. [ABSTRACT FROM AUTHOR]

Lindera glauca with rich resource and fruit terpene has emerged as potential material for utilization in China, but different germplasms show a variation for essential oil content and volatile profiling. This work aimed to determine key regulators (enzymes or transporters) and unravel mechanism of governing high production of essential oil of L. glauca fruit (EO-LGF). Temporal analysis of fruit growth and EO-LGF accumulation (yield, volatile compounds and contents) during development revealed a notable change in the contents of EO-LGF and its 45 compounds in developing fruits, and the major groups were monoterpene and sesquiterpene, showing good antioxidant and antimicrobial activities. To highlight molecular mechanism that govern such difference in terpene content and compound in developing fruits, Genome-wide assay was used to annotate 104 genes for terpene-synthesis pathway based on recent transcriptome data, and the comparative associations of terpene accumulative amount with gene transcriptional level were conducted on developing fruits to identify some crucial determinants (enzymes and transporters) with metabolic regulation model for high-quality terpene accumulation, involving in carbon allocation (sucrose cleavage, glycolysis and OPP pathway), metabolite transport, isoprene precursor production, C 5 -unit formation (MEP and MVA pathways), and mono−/sesqui-terpene synthesis. Our findings may present strategy for engineering terpene accumulation for utilization. • Essential oil with 45 volatile compounds in response to fruit development • The first global assay of genes for terpene-biosynthetic enzymes and transporters • Dynamic profile assay of terpene and gene transcript to identify key determinants • Enzyme/transporter-mediated metabolic regulatory model for high terpene production • Great potential of Lindera glauca essential oil to serve as novel natural agent [ABSTRACT FROM AUTHOR]

Background. Oil-tea Camellia is a very important edible oil plant widely distributed in southern China. Tea oil extracted from the oil-tea Camellia seeds is beneficial to health and is considered as a health edible oil. We attempt to identify genes related to fatty acid biosynthesis in an oil-tea Camellia seed kernel, generated a comprehensive transcriptome analysis of the seed kernel at different developmental stages, and explore optimal picking time of fruit. Material and Methods. A gas chromatography-mass spectrometer was used to detect the content of various fatty acids in samples. Transcriptome analysis was performed to detect gene dynamics and corresponding functions. Results. Multiple phenotypic data were counted in detail, including the oil content, oleic acid content, linoleic acid content, linolenic acid content, fruit weight, fruit height, fruit diameter, single seed weight, seed length, and seed width in different developmental stages, which indicate that a majority of indicators increased with the development of oil-tea Camellia. The transcriptomics was conducted to perform a comprehensive and system-level view on dynamic gene expression networks for different developmental stages. Short Time-series Expression Miner (STEM) analysis of XL106 (the 6 time points) and XL210 (8 time points) was performed to screen related fatty acid (FA) gene set, from which 1041 candidate genes related to FA were selected in XL106 and 202 related genes were screened in XL210 based on GO and KEGG enrichment. Then, candidate genes and trait dataset were combined to conduct correlation analysis, and 10 genes were found to be strongly connected with several key traits. Conclusions. The multiple phenotypic data revealed the dynamic law of changes during the picking stage. Transcriptomic analysis identified a large number of potential key regulatory factors that can control the oil content of dried kernels, oleic acid, linoleic acid, linolenic acid, fresh seed rate, and kernel-to-seed ratio, thereby providing a new insight into the molecular networks underlying the picking stage of oil-tea Camellia, which provides a theoretical basis for the optimal fruit picking point. [ABSTRACT FROM AUTHOR]

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Background: Lycium barbarum and L. ruthenicum have been used as traditional medicinal plants in China and other Asian counties for centuries. However, the molecular mechanisms underlying fruit development and ripening, as well as the associated production of medicinal and nutritional components, have been little explored in these two species. Results: A competitive transcriptome analysis was performed to identify the regulators and pathways involved in the fruit ripening of red wolfberry (L. barbarum) and black wolfberry (L. ruthenicum) using an Illumina sequencing platform. In total, 155,606 genes and 194,385 genes were detected in red wolfberry (RF) and black wolfberry (BF), respectively. Of them, 20,335, 24,469, and 21,056 genes were differentially expressed at three different developmental stages in BF and RF. Functional categorization of the differentially expressed genes revealed that phenylpropanoid biosynthesis, flavonoid biosynthesis, anthocyanin biosynthesis, and sugar metabolism were the most differentially regulated processes during fruit development and ripening in the RF and BF. Furthermore, we also identified 38 MYB transcription factor-encoding genes that were differentially expressed during black wolfberry fruit development. Overexpression of LrMYB1 resulted in the activation of structural genes for flavonoid biosynthesis and led to an increase in flavonoid content, suggesting that the candidate genes identified in this RNA-seq analysis are credible and might offer important utility. Conclusion: This study provides novel insights into the molecular mechanism of Lycium fruit development and ripening and will be of value to novel gene discovery and functional genomic studies. [ABSTRACT FROM AUTHOR]

Background. Quantitativereal-time reverse transcriptase polymerase chain reaction is the common method to quantify relative gene expression. Normalizating using reliable genes is critical in correctly interpreting expression data from qRT-PCR. Euscaphis konishii is a medicinal plant with a long history in China, which has various chemical compounds in fruit. However, there is no report describing the selection of reference genes in fruit development of Euscaphis konishii. Methods. We selected eight candidate reference genes based on RNA-seq database analysis, and ranked expression stability using statistical algorithms GeNorm, NormFinder, BestKeeper and ReFinder. Finally, The nine genes related to the anthocyanin synthesis pathway of Euscaphis konishii were used to verify the suitability of reference gene. Results. The results showed that the stability of EkUBC23, EkCYP38 and EkGAPDH2 was better, and the low expression reference genes (EkUBC23 and EkCYP38) were favourable for quantifying low expression target genes, while the high expression reference gene (EkGAPDH2) was beneficial for quantifying high expression genes. In this study, we present the suitable reference genes for fruit development of Euscaphis konishii based on transcriptome data, our study will contribute to further studies in molecular biology and gene function on Euscaphis konishii and other closely related species. [ABSTRACT FROM AUTHOR]

Attempts had been made to provide evidences insight into the pattern and physiological mechanism of sweet cherry fruit dropping. However, the fruit abscission pattern and mechanism underlying the heavy fruit dropping in insufficient chilling regions, especially the roles of interior factors including carbohydrates and hormones in abnormal fruit dropping, has not yet been better understood so far. Currently, four five-year-old sweet cherry (Prunus avium L.) cultivars, i.e., 'Santina', 'Brooks', 'Sparkerry' and 'Black Pearl', which were grown in Weining County (104.28° E, 26.86° N), Guizhou province of China, were used as materials to elucidate the physiological fruit abscission pattern of sweet cherry and the chief interior causes for the heavy fruit dropping in insufficient chilling region of China. It was explicated that the abscission pattern of sweet cherry fruit somewhat varied among the cultivars in a low chilling region. Higher rate of pollen germination and longer polar axis length was proved to be remarkably beneficial to fruit development. A comparative anatomic dissection of fruit stalk between the retention and dropped fruit elaborated that there is obvious difference in the microstructure with the progress of fruit development. The discrepancy was mostly showed by the cell space of abscission zone tissues for the dropped fruit enlarged gradually and was remarkably larger than that of the retention ones. Furthermore, the imbalance of endogenous hormones in fruit is a crucial trigger for fruit dropping with the progress of fruit development, particularly the active ZR, GA, as well as IAA levels in fruit are positively correlated to fruit setting, conversely, ABA played a negative role in fruit retention. Moreover, carbohydrate contents in retention fruit were considerably higher than that of the dropped ones during the fruit development, however, the contents of glucose and fructose in dropped fruit did not reach the required threshold which was possibly a vital cause leading to fruit abscission. [ABSTRACT FROM AUTHOR]

An experiment was conducted in the year 2018 at ICAR-NRC on Litchi to investigate the effect of different pollen grain sources on fruit set and fruit retention in 'Bedana' litchi. The enclosed flower panicles of 'Bedana' were hand pollinated with male (M2) pollen from 'Shahi', 'China', 'Rose Scented', 'SwarnaRoopa' and 'Bedana' flowers. The current season's pollen as well as one year old pollen stored at 4 °C was used for pollination. The numbers of fruit set and fruit retention per panicle were determined throughout the fruit development period starting from pollination to harvest on a weekly basis. Initial fruit set was higher in all cross-pollination with pollen sources 'Shahi', 'China' 'Rose Scented' and 'Swarna Roopa' compared to self-pollination with pollen 'Bedana'. The final fruit retention in 'Bedana' was higher with all sources used for cross-pollination whereas there was no fruit retention in self-pollination where "Bedana" was used as source of pollen grains. Results indicated that cross-pollination in 'Bedana' litchi is essential for fruit set and fruit retention. The limited number of initial fruit set resulted in high retention of fruits (1.64% and 1.69%) in 'Bedana' with current season and one year old pollen of 'SwarnaRoopa'. It indicated that number of female flower should be restricted in a panicle for further pollination which would ensure good fruit retention at harvest. [ABSTRACT FROM AUTHOR]

Although unconventional water can be applied as an alternative for agricultural production, inappropriate irrigation water quality may adversely affect the soil crop system. Thus, field experiments under mulched drip irrigation were performed over three years (2017–2019) to investigate the impacts of three salinity (1, 2 and 3 g·L−1) with five Na:Ca molar ratios (1, 3, 5, 7 and 9) of irrigation water on tomato plant growth, fruit development, soil salt accumulation and soil sodium adsorption ratio. The results showed that, with irrigation water salinity of 1 g·L−1, the critical value of irrigation water Na:Ca molar ratio for the high yield and dry matter accumulation was 3 and 5, respectively, while the threshold for high concentration of vitamin C and lycopene was 7. With the irrigation water salinity of 2 g·L−1 and 3 g·L−1, the Na:Ca molar ratio threshold for a high concentration of soluble solids, vitamin C and lycopene was 5. Moreover, considering the increase of plant nitrogen and phosphorus accumulation, the decrease of sodium adsorption ratio at 40–100 cm soil layer inside the film, and the diminution of soil salt accumulation at 0–100 cm layer, irrigation water with higher salinity and lower Na:Ca molar ratio was more proper than that with lower salinity and higher Na:Ca molar ratio. Based on the multi-objective optimization with entropy-weight TOPSIS method, irrigation water with a salinity level at 2 g·L−1 and the Na:Ca molar ratio at 5 was suggested for tomato planting in the Yinbei Yellow River Irrigation District, China. • The effects of irrigation water Na:Ca molar ratio on soil-crop system was indicated. • Critical values of Na:Ca molar ratio for high-yield and good-quality were recognized. • Appropriate water salinity and Na:Ca molar ratio for tomato cultivation in Ningxia. [ABSTRACT FROM AUTHOR]

Lycium chinense is an important medicinal plant in the northwest of China. Flavonoids are the major pharmacological components of L. chinense fruits. However, flavonoid metabolism during fruit development of L. chinense remains to be studied. Here, we analyzed the change of flavonoid contents, enzyme activity, and gene expression during fruit development of L. chinense. We found that flavonoids, anthocyanins, and catechins are the most important components of L. chinense fruits. Flavonoid content was increased with fruit development and was high at the late developmental stage. PAL, CHS, and F3H enzymes played a significant role in flavonoid accumulation in fruits. Transcriptomic analysis showed that anthocyanin pathway, flavonol pathway, flavonoid biosynthesis, and phenylpropanoid synthesis pathway were the major pathways involved in flavonoid metabolism in L. chinense. Gene expression analysis indicated that PAL1 and CHS2 genes were critical for flavonoid metabolism in L. chinense fruits. These discoveries help us understand the dynamic changes in flavonoids during fruit development and enhance the use of L. chinense fruits. [ABSTRACT FROM AUTHOR]

Litchi chinensis Sonn. cv. ‘Feizixiao’ is commonly cultivated in orchards in Hainan Island of tropical China; however, its commodity value was reduced due to the non-synchronized pericarp coloration and low aril nutritional quality. 5-Aminolevulinic acid (5-ALA), a key precursor in the biosynthesis of porphyrin compounds, is known to be an effective plant growth regulator for improving fruit coloration and nutritive quality. In this study, 5-ALA solution was exogenously sprayed at concentrations of 80, 100, 120, 140, and 160 mg L −1 on the fruit peel of ‘Feizixiao’ litchi at 45 days after full bloom. Fruit growth, coloration and nutritive quality were determined. The treatment with 5-ALA at 80 mg L −1 maintained the vitamin C content of the aril at a higher level than that of the control. The application of 5-ALA significantly increased fruit length and diameter compared with that of the control, but there was no significant effect on the titratable acid content. At 20 days after 5-ALA treatment, anthocyanin contents of the pericarp were significantly higher and the hue angle (h°) was significantly lower than those of the control. Redundancy analysis showed that 5-ALA improved the skin color of ‘Feizixiao’ litchi by promoting anthocyanin accumulation, but not chlorophyll degradation, in the pericarp during the time of harvest. This study showed that 5-ALA significantly improved the skin color at harvest time and fruit quality by promoting the growth of fruit, delaying the degradation of soluble solids, increasing the edible rate, and shortening the interval of aril development and skin coloration. [ABSTRACT FROM AUTHOR]

• 38 phenotypes including phenological records, flower and fruit traits were evaluated among 1009 Chinese cherry accessions. • An index system for Chinese cherry DUS testing was firstly established by statistically phenotyping 26 traits. • The phenotypic variations mainly attributed to the fruit weight, size, firmness, pit weight, titratable acid and total soluble solid. Chinese cherry [ Cerasus pseudocerasus (Lindl.) G.Don], native to China, is an economically important tetraploid fruiting cherry species. In order to perfect the cherry germplasm descriptors system, and effectively rate on characters in DUS (distinctness, uniformity, stability) testing, we comprehensively investigated and analyzed 38 Chinese cherry phenotypes, including phenological records, flower, pit and main fruit quality traits, of 59 germplasm collections and 950 individuals from four cross F 1 populations. An index system for Chinese cherry DUS test was firstly established by statistically phenotyping 26 traits. The results showed that the fruit development period ranged from 43 to 69 days, which could be well applied to evaluate the maturity time and were divided into five ratings. Thirteen qualitative characters, including two in flower, five in fruit, and six in pit, were classified into two to six ratings, respectively. Significant variations were detected among these characters, with Shannon-Wiener indexes ranging from 0.37 to 1.49. The average coefficient of variation was 20.94% ranging from 5.48 to 76.56% among 22 quantitative traits. The fruit weight, size, firmness, pit weight, titratable acid and soluble solid mainly contributed to the phenotypic variations by principal component analysis. Eight fruit and pit characters, which were in accordance with the normal distribution, were rating by the least significant difference method, while four fruit characters, being out of the normal distribution, were rating by the range method. The establishment of phenotyping protocol will enrich cherry descriptors system and DUS test guideline, and provide important basis for marker-assisted breeding in Chinese cherry. Graphical abstract [Display omitted]. [ABSTRACT FROM AUTHOR]

Abstract: Fossil fruits of Schima (Theaceae) and seeds of Toddalia (Rutaceae) have been described as Schima nanlinensis sp. nov. and Toddalia nanlinensis sp. nov. respectively, from the Miocene of Nanlin Formation in Longchuan Basin, Dehong Autonomous Prefecture, Yunnan Province, China. The former are 5-loculed capsules with loculicidal dehiscence and remains of calyx at the base, as well as reniform flat seeds, while the latter are boat-shaped seeds with tegmen that is composed of thin-walled cells with fine criss-crossed spiral lignifications. The genus Schima Reinwardt ex Blume is known from the Palaeogene and Neogene of Germany and Austria, but today it is confined to South, East and Southeast Asia. Schima nanlinensis sp. nov. represents the first fossil record of the genus in Asia. The genus Toddalia, known to have existed in Europe from Eocene to Pliocene, is now widely distributed from Africa to Asia. Toddalia nanlinensis sp. nov. is the first fossil record of the genus in Asia and suggests that the genus probably extended the geographic distribution from Europe to Asia at least in the Miocene. [Copyright &y& Elsevier]

Changes in 3-alkyl-2-methoxypyrazines (MPs) in Cabernet Sauvignon (Vitis vinifera) and Zuoshanyi (Vitis amurensis Rupr.) grapes from different areas of North China were monitored throughout berry development in 2010. One kind of MP, 3-isobutyl-2-methoxypyrazine (IBMP), was detected in Cabernet Sauvignon grapes, and its content in grapes from Gaotai County was much higher than in those from the other two producing areas at the stages of véraison and maturity. Zuoshanyi grapes contained much more IBMP than Cabernet Sauvignon grapes, and an additional kind of MP, 3-isopropyl-2-methoxypyrazine (IPMP), was also detected in developing Zuoshanyi grapes. Abundant MPs might explain the stronger 'green' and 'herbaceous' characters in Zuoshanyi grapes. MPs accumulated in the early developmental stage in both Zuoshanyi and Cabernet Sauvignon grapes and rapidly decreased in the later stages. Delays in the time of harvest for Cabernet Sauvignon in Gaotai County and Zuoshanyi in Beijing are suggested in order to reduce MPs levels and the herbaceous character of these grapes. [ABSTRACT FROM AUTHOR]

Abstract: Knowledge of genetic divergence among inbred lines is essential for cross breeding. The objectives of this study were to (1) analyze the level and character of genetic diversity in C. moschata accessions, and (2) classify the genetic divergence among inbred lines in C. moschata to assist in selection of parent genotypes for genetic improvement. Twenty agronomic characters were investigated and rich diversities were demonstrated among 39 inbred lines of C. moschata from China. Various degree correlations among these characters made it possible to summarize the diversities of the twenty characters into 3 major principal components: leaf, fruit and flesh quality factor. Forty-one inbred lines of pumpkin were clustered into four groups based on principal component data, which is more distinct for classification that based on the original data of the 20 characters. However, parent inbred lines whose hybrids displayed significant heterosis in fruit weight, soluble solid and fruit shape were located in different clusters or sub-clusters based on standardized original data. It was suggested that genotypes in the same clusters may represent members of one heterotic group. [Copyright &y& Elsevier]

Background and Aims: Diptychocarpus strictus is an annual ephemeral in the cold desert of northwest China that produces heteromorphic fruits and seeds. The primary aims of this study were to characterize the morphology and anatomy of fruits and seeds of this species and compare the role of fruit and seed hetermorphism in dispersal and germination. [ABSTRACT FROM PUBLISHER]

Abstract: The effects of ultrasound treatment on fruit decay and physiological quality of strawberry fruit were investigated. Freshly harvested strawberries were treated with 0, 25, 28, 40 or 59kHz ultrasound at 20°C for 10min and then stored at 5°C for 8days. The results showed that 40kHz ultrasonic treatment significantly reduced decay incidence and numbers of microorganism. Ultrasound treatment also inhibited the decrease of firmness and maintained significant higher levels of total soluble solids (TSS), total titratable acidity (TA) and vitamin C. Treatments with 25 and 28kHz ultrasound had no significant effects on fruit decay and quality deterioration of strawberry fruit. Thus, ultrasound treatment has potential to extend shelf-life and maintain quality in strawberry fruit. [Copyright &y& Elsevier]

Abstract: Emergy and economic methods were used to evaluate and compare a traditional tropical fruit cultivation system, for bananas, and three newly introduced fruit cultivation systems, for papaya, guava and wampee, on reclaimed wetlands of the Pearl River Estuary, China. The goal of this study was to apply ecological engineering principles to fruit production system designs to maximize total emergy benefits and sustainability. The evaluations considered input structure, production efficiency, environmental impacts, economic viability and sustainability. The market effects on emergy exchange were assessed both for purchasing the inputs to production and for selling the fruit. These market effects were also considered in the evaluation of sustainability by using the Emergy Index for Sustainable Development (EISD), which was evaluated with and without taking the change in natural capital (i.e., soil organic matter) into consideration. The results showed that all three of the newly introduced systems are much more sustainable than the traditional banana production system. The guava production system had the highest value of the Emergy Sustainability Index (ESI=0.40). The high price of wampee gave it the highest economic yield/cost ratio (4.87) and EISD (0.73). Emergy and economic evaluations are complementary methods, with emergy analysis shedding more light on environmental support and impacts of the production systems not considered in the market value, and economic analysis focusing on the effects of markets on fruit production. The Emergy Exchange Ratio (EER) was proposed as a bridge between emergy and economic evaluations for specific systems and/or processes. [Copyright &y& Elsevier]

We report the release of a new, largefruited kiwifruit cultivar, 'White', selected from Actinidia eriantha. Fruit of 'White' had a mean fruit weight of 94 g, and a maximum fruit weight of 132 g at harvest in Zhejiang, China. The mean fruit weight is c. 2-4 times that of wild A. eriantha fruit found in Zhejiang and nearly 4 times that of 'Bidan', the only other registered A. eriantha cultivar. The fruit are oblong and the peelable skin is covered with dense white hair. The flesh of ripe fruit is green and has good eating quality but little juice. The soluble solids concentration was 14.7%, total sugar 9%, total acid 1.24%, and vitamin C 628.37 mg/100 g fresh weight. Its harvest season is during the last 10 days of October in the south of Zhejiang province, which is c. 2 weeks later than A. deliciosa 'Bruno' in the same area. It is sometimes referred to as the "banana kiwifruit" because the skin is easy to peel. This new cultivar has been registered in China (PVR No. CNA20050673.0). [ABSTRACT FROM AUTHOR]