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Genetic variants of SARS-CoV-2 have been emerging and circulating in many places across the world. Rapid detection of these variants is essential since their dissemination can impact transmission rates, diagnostic procedures, disease severity, response to vaccines or patient management. Sanger sequencing has been used as the preferred approach for variant detection among circulating human immunodeficiency and measles virus genotypes. Using primers to amplify a fragment of the SARS-CoV-2 genome encoding part of the Spike protein, we showed that Sanger sequencing allowed us to rapidly detect the introduction and spread of three distinct SARS-CoV-2 variants in two major Brazilian cities. In both cities, after the predominance of variants closely related to the virus first identified in China, the emergence of the P.2 variant was quickly followed by the detection of the P1 variant, which became dominant in less than one month after it was first detected., (Copyright © 2021. Published by Elsevier Inc.)

In the city of Rio de Janeiro (Brazil), the districts located in the northern area are considered the most critical regarding ozone levels year-round. In this study, the potential factors that contribute to high levels of ozone in the district of Irajá were investigated. The obtained results clearly showed that, in spite of the high correlation of ozone concentrations with wind speed and temperature, ozone episodes depend on the ratios of volatile organic compounds (VOCs) to nitrogen oxides (NO x ) rather than on the individual VOC and NO x levels, as expected for the VOC-controlled condition typical of the urban area of Rio de Janeiro. Moreover, high VOCs/NO x ratios are highly dependent on the transport of air masses. When pollutants are transported from urban areas with heavy vehicular flux and high NO x concentrations, ozone levels are reduced. When air masses are transported from the industrial petrochemical area, NO x levels are relatively low, and ozone episodes are frequent.

When Europeans arrived in America, the Brazilian Atlantic rainforest covered approximately 1,290,000 km 2 . Now, only 8% of the biome's original vegetation remains. One of the largest areas is Tijuca Forest National Park. In this work, the concentrations of 13 carbonyl compounds in an isolated area inside Tijuca Forest, in an urban park with primary and secondary vegetation (Gericinó Natural Park) and in two typical urban areas (Tijuca District and the city of Nilópolis) were determined. The main compounds were formaldehyde and acetaldehyde. The formaldehyde mean concentrations were 0.98 ± 1.00, 1.27 ± 1.67, 3.09 ± 1.60 and 2.33 ± 2.17 μg m -3 for Tijuca Forest, Gericinó Natural Park, Tijuca District and the city of Nilópolis, respectively. The mean acetaldehyde concentrations were, for the same locations, 0.93 ± 1.05, 2.94 ± 2.54, 2.78 ± 0.91 and 5.48 ± 1.90 μg m -3 . The results indicate that the compounds measured within the forest are transported from the city and that the trees play an important role in removing air pollutants. In contrast, the Gericinó protected area is heavily affected by urban emissions, and its capacity to dilute or absorb pollutants is low because of the sparse vegetation.

Administrative data show high vaccination coverage rates in Brazil, but there is no assessment of the validity and timeliness of dose administration, or whether the vaccination schedule is complete. This study assessed timely and updated coverage rates in children 12 to 24 months of age. This was a longitudinal population-based study in Araraquara, São Paulo State, a predominantly urban medium-sized municipality, using the Juarez System, an electronic immunization registry (EIR). Coverage rates were assessed in 49,741 children born from 1998 to 2013, a period in which five different vaccination schedules were used. Trends were estimated with the Prais-Winsten linear regression method. Updated coverage of the complete schedule varied from 79.5% to 91.3% at 12 months and from 75.8% to 86.9%, at 24 months. Timely coverage (all doses applied at the recommended ages, with no delays) ranged from 53.3% to 74% at 12 months and from 36.7% to 53.8% at 24 months. There was an upward trend in updated coverage at 24 months. The delays in relation to recommended age increased starting at six months and appeared to relate more to age than to the number of doses in the schedule. The proportion of invalid and late doses was lower than in other studies. Despite the increase in the number of doses in the vaccination schedule, the study showed high updated coverage rates and higher timely coverage than reported in the national and international literature; however, more effort is needed to increase timeliness. EIR proved relevant for assessing and monitoring vaccination coverage with more accurate analyses.