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This paper presents two datasets about college students' subjective well-being and mental health in a developing country. The first data set of this report offers a diagnosis of the prevalence of self-reported symptoms associated with stress, anxiety, depression, and overall evaluation of subjective well-being. The study uses validated scales to measure self-reported symptoms related to mental health conditions. To measure stress, the study used the Perceived Stress Scale (PSS-10) and the 7-item Generalized Anxiety Disorder Scale (GAD-7) to measure symptoms associated with anxiety (GAD-7), and the 9-item Patient Health Questionnaire (PHQ-9) to measure symptoms associated with depression. This diagnosis was collected in a college student sample of 3052 undergrad students in 2022 at a medium-sized university in Colombia. The second dataset reports the evaluation of a positive education intervention implemented in the same university. The Colombian Minister of Science and Technology financed the intervention to promote strategies to mitigate the consequences on college students' well-being and mental health after the pandemic. The program evaluation data cover two years (2020–2022) with 193 college students in the treatment group (students enrolled in a class teaching evidence-based interventions to promote well-being and mental health awareness) and 135 students in the control group. Data for evaluation include a broad array of variables of life satisfaction, happiness, negative emotions, COVID-19 effects, relationships valuations, and habits and the measurement of three scales: The Satisfaction with Life Scale (SWLS), a brief measurement of depressive symptomatology (CESD-7), and the Brief Strengths Scale (BSS). Dataset: Diagnosis dataset: https://data.mendeley.com/datasets/bytb22nf7m/1 (accessed on 21 February 2024), Available at Mendeley Data; Program evaluation dataset: https://data.mendeley.com/datasets/c4nmk6h3kj (accessed on 21 February 2024), Available at Mendeley Data. Dataset License: CC BY-NC-SA [ABSTRACT FROM AUTHOR]