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Introduction: Introduction: professional soccer both in the local setting and in other countries is highly competitive, and those who practice must have specific morphological, anthropometric, and body composition characteristics, in addition to constant monitoring of nutritional and training interventions. Currently, the gold-standard criterion for the evaluation of body composition is Dual Energy X-ray Absorciometry (DXA), which is a costly laboratory method with limited use for many professionals. Knowing which field methods obtain similar results to this would allow a better interdisciplinary approach, which could have a positive impact on sports performance. The objective of this study was to evaluate the accuracy of different formulas for predicting fat percentage and fat mass, as compared to DXA, in Colombian soccer team players. Methods: a cross-sectional, analytical study using convenience sampling. A total of 79 professional male soccer players, belonging to 4 Colombian first and second division clubs, were included. Thirty anthropometric variables were measured, wherewith a descriptive analysis was performed using the SPSS v.21 program, and a procedure with analytical scope was carried out to establish concordance indices between different measurements using the Bland and Altman method. This statistical process was performed using the library (BlandAltmanLeh) of the statistical program "R". Results: average age was 23 ± 4.4 years, and the percentage of body fat was estimated using six equations: Jackson and Pollock (7.20 ± 2.58 %), Yuhasz as modified by Carter (7.52 ± 8.50 %), Reilly (10.04 ± 1.43 %), Faulkner (11.23 ± 11.90 %), Pariskova and Buskova (11.08 ± 16.06 %), and Durnin and Womersley (12.41 ± 20,10 %), in addition to the calculation of fat percentage for fat mass using the five-component method (13.17 ± 2.86 %). The percentage of body fat that showed the lowest intermethod difference was fractionation by five components (0.54 ± 3.56), followed by Durnin and Womersley (0.66 ± 3.52). Conclusion: the calculations of fat percentage using the fractionation of 5 components method and the Durnin and Womersley equation were closest to the results obtained by the gold-standard method (DXA) in soccer players of Colombian professional teams. [ABSTRACT FROM AUTHOR]