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Cold seep ecosystems can support enormous biomasses of free-living and symbiotic chemoautotrophic organisms that get their energy from the oxidation of methane or sulfide. Most of this biomass derives from animals that are associated with bacterial symbionts, which are able to metabolize the chemical resources provided by the seeping fluids. Often these systems also harbor dense accumulations of non-symbiotic megafauna, which can be relevant in exporting chemosynthetically fixed carbon from seeps to the surrounding deep sea. Here we investigated the carbon sources of lithodid crabs (Paralomis sp.) feeding on thiotrophic bacterial mats at an active mud volcano at the Costa Rica subduction zone. To evaluate the dietary carbon source of the crabs, we compared the microbial community in stomach contents with surface sediments covered by microbial mats. The stomach content analyses revealed a dominance of epsilonproteobacterial 16S rRNA gene sequences related to the free-living and epibiotic sulfur oxidiser Sulfurovum sp. We also found Sulfurovum sp. as well as members of the genera Arcobacter and Sulfurimonas in mat-covered surface sediments where Epsilonproteobacteria were highly abundant constituting 10% of total cells. Furthermore, we detected substantial amounts of bacterial fatty acids such as i-C15∶0 and C17∶1ω6c with stable carbon isotope compositions as low as −53‰ in the stomach and muscle tissue. These results indicate that the white microbial mats at Mound 12 are comprised of Epsilonproteobacteria and that microbial mat-derived carbon provides an important contribution to the crab's nutrition. In addition, our lipid analyses also suggest that the crabs feed on other 13C-depleted organic matter sources, possibly symbiotic megafauna as well as on photosynthetic carbon sources such as sedimentary detritus. [ABSTRACT FROM AUTHOR]

Mud extrusion is frequently observed as a dewatering phenomenon in compressional tectonic settings such as subduction zones. Along the Middle American Trench, several of these features have been recently discovered. This paper presents a heat flow study of actively venting Mound Culebra, offshore Nicoya Peninsula, and is complemented by data from geophysical surveys and coring. The mud diapir is characterised by methane emission and authigenic carbonate formation at its crest, and is composed of overconsolidated scaly clays and clast-bearing muds. Compared with the conductive background heat flow, the flux through the mud dome is elevated by 10–20 mW/m2, possibly related to advection of heat by fluids rising from greater depth. Decreased chlorinity in the pore waters from gravity cores may support a deep-seated fluid origin. Geothermal measurements across the mound and temperature measurements made with outriggers on gravity corers were corrected for the effects of thermal refraction, forced by the topography of the mound. Corrected values roughly correlate with the topography, suggesting advection of heat by fluids rising through the mound, thereby generating the prominent methane anomaly over the dome and nurturing vent biota. However, elevated values occur also to the southeast of the mound. We believe that the overconsolidated clays and carbonates on the crest form an almost impermeable lid. Fluids rising from depth underneath the dome are therefore partially channelled towards the flanks of the mound. [Copyright &y& Elsevier]