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Genetic characterization strawberry cultivars (Fragaria ananassa Duch.) existing in Cuba is of great interest because it allows to get basic information necessary for the design of conservation strategies of this germplasm for future crop improvement programs in the country, in order to select better adapted cultivars to our soil and climate. The aim of this study is to determine the variability in seven strawberry genotypes grown in different parts of the island by using biochemical markers. The analysis was performed for esterase, carbonic anhydrase, acid phosphatase and malate dehydrogenase isoenzyme systems. The total polymorphism degree of studied systems was high, at 89 %, been the most polymorphic malate dehydrogenase with 100 %. The highest level of polymorphism among cultivars was observed in the cultivar 'Missionary' with 81,2 % and the lowest in cultivar of Villa Clara with 32,4 %. Built a matrix absence - presence of bands, which was processed by the Past statistical software package version 2,14, was used Jaccard index and the UPGMA method to obtain the dendrogram. These results allowed to determine the degree of variability present in the strawberry cultivars exist in Cuba, information that can be exploited by breeding programs of the crop. [ABSTRACT FROM AUTHOR]

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Para caracterizar genéticamente la cabra Criolla Cubana, como parte del proyecto para la conservación y mejora de esta raza, declarada en peligro de extinción en el año 2003, se recolectaron muestras de pelo de 40 animales. De estos, 33 % procedían del único núcleo genético de la raza existente en Cuba, localizado en la Empresa Genética «Manuel Fajardo», en Jiguaní, Granma. El resto pertenecía a varias fincas, cooperativas de producción agropecuarias y otras explotaciones de las provincias Granma, Santiago de Cuba y Holguín. Se utilizó un panel de 26 microsatélites, recomendados por la FAO y la Sociedad Internacional de Genética Animal (ISAG) para estudios de diversidad genética de la especie caprina. La amplificación de los mismos se realizó mediante la reacción en cadena de la polimerasa (PCR) y los fragmentos amplificados, separados por electroforesis en geles de poliacrilamida. Se calcularon las frecuencias alélicas, las heterocigosidades esperadas (He) y las observadas (Ho) y el contenido de información polimórfica (PIC), así como el equilibrio Hardy-Weinberg (EHW) por marcador. El número medio de alelos encontrado por locus fue de 6.38. La totalidad de los loci estudiados resultaron polimórficos en la raza, y 77 % de ellos mostró un contenido de información polimórfica (PIC) elevado. Esto, unido a la presencia de más de 58 % de individuos heterocigotos, evidencia que la cabra Criolla Cubana es un ente racial con identidad propia, que posee una elevada diversidad genética. [ABSTRACT FROM AUTHOR]

Copyright of Revista Colombiana de Biotecnología is the property of Universidad Nacional de Colombia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Molecular characterization of 37 coconut (Cocos nucifera L.) ecotypes from a natural population of Baracoa municipality, Guantánamo province, was carried out by using Inverse Sequence Tagged Repeat (ISTR) technique. This method was able to detect the high polymorphism (93%) of the evaluated population showing its usefulness for studies on molecular diversity in coconut. The identification percentage was also relatively high (Pi= 86%) suggesting that the selected primer combinations could be used for the ecotype identification studies on coconut populations of Baracoa. Evaluation of the diversity in different coconut ecotypes by means of ISTR markers showed a low level of variability within the population studied in Baracoa region. These data suggest that coconut populations in this zone have been originated from coconuts with a relatively small quantity of seeds or even from introduced materials from populations of low diversity. [ABSTRACT FROM AUTHOR]

A study was carried out in order to make a deep analysis of the current knowledge of cassava's (Manihot esculenta Crantz) genetic diversity, and to evaluate its phylogenetic relationship with relatives cultivated in Africa, and South and Central America in order to facilitate a sustainable management of the genetic resources available in Cuba. A number of 94 cultivars from the Cuban cassava germplasm were studied according to their genetic or economic importance; besides, 54 clones from Africa and America, and 13 genotypes of genetic interest were incorporated. Diversity and genetic differentiation analysis were developed from data of 34 microsatellite markers (SSR). Genetic diversity indexes evidenced the high polymorphism observed for the tested microsatellites. The plant material coming from Cuba showed the highest average number of allele per locus with 5.8 and like Guatemala, it illustrated 100% polymorphic loci. The highest mean heterozygosity indexes (Ho) are presented by Cuba and Tanzania. The mean rate of heterozygous individuals observed (Ho) was high (0.5918 ±0.0351). These results, the first reported in Cuba on cassava, offer an important contribution to the genetic breeding program and to the sustainable management of the cassava genetic diversity in Cuba and in the Caribbean area. [ABSTRACT FROM AUTHOR]