1. Probing protein conformation electrochemically.
- Author
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Sage, Linda
- Subjects
- *
ELECTROCHEMISTRY , *TRYPTOPHAN , *PROTEIN conformation , *OSMIUM tetroxide , *BIPYRIDINE , *STREPTAVIDIN , *OXIDATION-reduction reaction , *VOLTAMMETRY - Abstract
This article discusses an electrochemical method for detecting tryptophan inside proteins. Tryptophans are often buried in the protein core, but when the protein unfolds, they emerge. Determining if tryptophan residues are exposed can show protein conformation when exposed to another protein or ligand. Researcher, Miroslav Fojta, at the Czech Academy of Sciences Brno and others at Masaryk University in the Czech Republic developed the procedure. They exposed osmium tetroxide-2,2'- bipyridine complex (Os,bipy) to avidin (or streptavidin) and measured the redox potential using a voltammetric cell. The research appears in the vol. 80, 2008 issue.
- Published
- 2008
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