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The Rozna Mine is one of the last active uranium mines in Europe. When the mine is closed and flooded, the natural groundwater flow pattern will be partly restored. Re-established groundwater flow system will be associated with an increase of groundwater discharge into draining rivers and streams. Since the groundwater inflows to streams can be contaminated by the mine water, the groundwater drainage characteristic of fractured aquifer should be carefully identified. Several methods of groundwater discharge zones identification were used including morphological analysis, thermometry, and electrical conductivity (EC) measurements. Stream temperatures and EC at more than 700 points in the area covering about 85 km2 were measured. The measurements were performed during winter period, when stream discharges were low and there was a maximum temperature contrast. There was a frequent presence of preferential discharge zones with resulting anomalous temperatures and electric conductivity values of stream water. The results show evident correlation of discharge zones with surface morphology and geological settings. Just like the aquifer discharge characteristics, the aquifer is strongly heterogeneous. The thermometry supported by measurement of EC proved to be a useful tool for large-scale investigation of groundwater flow and drainage in fractured aquifers. [ABSTRACT FROM AUTHOR]