1. Variability of the Chemical Composition and Bioactivity between the Essential Oils Isolated from Male and Female Specimens of Hedyosmum racemosum (Ruiz & Pav.) G. Don.
- Author
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Valarezo E, Morocho V, Cartuche L, Chamba-Granda F, Correa-Conza M, Jaramillo-Fierro X, and Meneses MA
- Subjects
- Allylbenzene Derivatives chemistry, Allylbenzene Derivatives isolation & purification, Anisoles chemistry, Anisoles isolation & purification, Anti-Bacterial Agents isolation & purification, Anti-Bacterial Agents pharmacology, Antioxidants isolation & purification, Antioxidants pharmacology, Arthrodermataceae drug effects, Arthrodermataceae growth & development, Benzothiazoles antagonists & inhibitors, Bicyclic Monoterpenes chemistry, Bicyclic Monoterpenes isolation & purification, Biphenyl Compounds antagonists & inhibitors, Cyclohexane Monoterpenes isolation & purification, Ecuador, Eugenol analogs & derivatives, Eugenol chemistry, Eugenol isolation & purification, Female, Gram-Negative Bacteria drug effects, Gram-Negative Bacteria growth & development, Gram-Positive Bacteria drug effects, Gram-Positive Bacteria growth & development, Humans, Magnoliopsida metabolism, Male, Microbial Sensitivity Tests, Picrates antagonists & inhibitors, Plant Leaves chemistry, Plants, Medicinal, Sesquiterpenes, Germacrane chemistry, Sesquiterpenes, Germacrane isolation & purification, Sex Factors, Sulfonic Acids antagonists & inhibitors, Anti-Bacterial Agents chemistry, Antioxidants chemistry, Cyclohexane Monoterpenes chemistry, Magnoliopsida chemistry, Oils, Volatile chemistry
- Abstract
Hedyosmum racemosum (Ruiz & Pav.) G. is a native species of Ecuador used in traditional medicine for treatment of rheumatism, bronchitis, cold, cough, asthma, bone pain, and stomach pain. In this study, fresh H. racemosum leaves of male and female specimens were collected and subjected to hydrodistillation for the extraction of the essential oil. The chemical composition of male and female essential oil was determined by gas chromatography-gas chromatography equipped with a flame ionization detector and coupled to a mass spectrometer using a non-polar and a polar chromatographic column. The antibacterial activity was assayed against five Gram-positive and two Gram-negative bacteria, and two dermatophytes fungi. The scavenging radical properties of the essential oil were evaluated by DPPH and ABTS assays. The chemical analysis allowed us to identify forty-three compounds that represent more than 98% of the total composition. In the non-polar and polar column, α-phellandrene was the principal constituent in male (28.24 and 25.90%) and female (26.47 and 23.90%) essential oil. Other main compounds were methyl chavicol, germacrene D, methyl eugenol, and α-pinene. Female essential oil presented a strong activity against Klebsiella pneumoniae (ATCC 9997) with an minimum inhibitory concentration (MIC) of 500 μg/mL and a scavenging capacity SC
50 of 800 µg/mL.- Published
- 2021
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