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Purpose: Bovine babesiosis causes morbidity in tropical and subtropical countries worldwide. The present study aimed to determine the seroprevalence of Babesia bigemina and B. bovis in cattle and water buffaloes in Menoufia province, where the second-highest population of bovines in Lower Egypt are raised. Materials and Methods: A total of 506 blood samples were collected from cattle (N = 262) and water buffaloes (N = 244) in Menoufia province, Egypt. Seroprevalences of B. bigemina and B. bovis in the samples were determined using recombinant Babesia antigen-specific enzyme-linked immunosorbent assays (ELISA). Results: In cattle, the seroprevalences of B. bigemina and B. bovis were 41.60 and 38.17% (37.40 and 35.88% for IgM and 9.54 and 6.11% for IgG), respectively, whereas those of water buffaloes were 35.66 and 31.97% (27.87 and 21.72% for IgM and 15.16 and 15.16% for IgG), respectively. Statistically significant changes in the seroprevalences of the two infective agents were recorded on the basis of region and season of sample collection. Conclusion: In conclusion, babesiosis is frequent and presents a threat of an epidemic among bovines in Menoufia province. In turn, control of bovine babesiosis is required because of its potential to detrimentally affect milk and meat production in Menoufia province. [ABSTRACT FROM AUTHOR]

• Theileria equi genotype E detected in Egypt for the first time. • New Babesia caballi genotype identified via phylogenetic analysis. • Babesia naoakii detected again in Egyptian camels, need investigation. • Babesia microti detected again in Egyptian camels, raises health concern. • Future focus: large-scale studies on piroplasmids in Egyptian camels. Piroplasmosis, a tick-borne disease affecting livestock, including camels, is caused by intracellular apicomplexan parasites belonging to the order Piroplasmida. Despite its importance, there's limited research on piroplasmosis among Egyptian camels. This study aimed to fill this gap by investigating tick-borne piroplasmids in camels from Cairo and Giza Governorates. Out of 181 blood samples collected between October 2021 and March 2022 from apparently healthy one-humped camels (Camelus dromedarius), PCR assays revealed a 41.4 % infection rate with various piroplasmids. Detected species included B. bovis (17.7 %), B. bigemina (12.2 %), B. caballi (8.3 %), B. naoakii (11.6 %), B. microti (1.7 %), T. equi (4.4 %), and Theileria spp. (28.7 %). Phylogenetic analysis revealed the first detection of T. equi genotype E in Egypt and identified a novel B. caballi genotype. Additionally, B. microti isolates were identified as the US-type. These findings shed lights on piroplasmosis among Egyptian camels, and provide valuable information for devising effective control strategies, especially B. microti , a pathogen with potential human health risks. [Display omitted] [ABSTRACT FROM AUTHOR]

Tick-borne diseases are of global economic importance, especially due to the costs associated with disease treatment and productivity losses in livestock. In this study, 244 livestock animals (cattle N = 92, buffaloes N = 86 and sheep N = 66) from Menoufia, Egypt were tested for Anaplasma, Ehrlichia and Babesia species using PCR. Results revealed detection of A. ovis (9.1%) in sheep while Anaplasma spp. (14.1%), A. marginale (15.2%) , B. bigemina (6.5%) and B. bovis (5.4%) in cattle. On the other hand, Anaplasma spp. (1.2%), A. marginale (1.2%) and B. bovis (1.2%), were detected in buffaloes. Significantly higher detection rates were observed in cattle for Anaplasma spp. (P =.020), A. marginale (P =.001) and B. bigemina (P =.022) than in buffaloes. Sequence analysis of Anaplasma spp. isolates from cattle, revealed A. platys- like strains. Phylogenetic analyses of the A. platys -like isolates revealed variation among the strains infecting cattle. The A. marginale buffalo isolate, on the other hand, showed some level of divergence from the cattle isolates. This study reports the first detection of A. ovis in sheep and A. platys- like strains in cattle in Menoufia and Egypt at large. The results of the current study provide valuable information on the epidemiology and genetic characteristics of tick-borne pathogens infecting livestock in Egypt. Unlabelled Image [ABSTRACT FROM AUTHOR]