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Human giardiasis is a common waterborne/foodborne parasitic disease worldwide, especially in developing countries. Prevalence and molecular identity of Giardia parasites are largely controversial. The present study was conducted to determine the occurrence of Giardia parasites and the genetic profile of circulating assemblage(s) in patients attended the outpatient clinic at Kafrelsheikh University hospital, Kafr El Sheikh Province, Egypt. A total of 318 patients, of different age and sex, referred to the clinic were subjected to fecal examination. Microscopic results revealed that 181/318 (56.9%) were positive for Giardia parasites. Multilocus genotyping by PCR/sequencing of beta-giardin (bg), triose phosphate isomerase (tpi), and glutamate dehydrogenase (gdh) genes of representative number of positive samples (65) revealed that assemblages A, B and mixed infections (A + B) occurred in 26/65 (40.0%), 32/65 (49.2%) and 10.8% (7/65) of the analyzed isolates, respectively. MLGs analysis indicated that assemblage A sequences clustered in two novel types of AII sub-assemblage. In assemblage B sequences, BIII was the predominant (22/23, 95.7%) sub-assemblage compared to BIV (1/23, 4.3%). Collectively, assemblage B MLGs displayed greater levels of genetic diversity compared to assemblage A. Our data indicate that assemblages A and B of G. duodenalis circulate in humans at Kafr El Sheikh Province, Egypt, and that high genetic diversity exists at the assemblage and/or sub-assemblage levels., (Copyright © 2019 Elsevier B.V. All rights reserved.)

In this study, 111 Cryptosporidium parvum IId isolates from several species of animals in China, Sweden, and Egypt were subtyped by multilocus sequence typing (MLST). One to eleven subtypes were detected at each of the 12 microsatellite, minisatellite, and single nucleotide polymorphism (SNP) loci, forming 25 MLST subtypes. Host-adaptation and significant geographical segregation were both observed in the MLST subtypes. A clonal population structure was seen in C. parvum IId isolates from China and Sweden. Three ancestral lineages and the same RPGR sequence were shared by these isolates examined. Therefore, the present genetic observations including the higher nucleotide diversity of C. parvum IId GP60 sequences in Western Asia, as well as the unique distribution of IId subtypes (almost exclusively found in Asia, Europe, and Egypt) and in combination with the domestication history of cattle, sheep, and goats, indicated that C. parvum IId subtypes were probably dispersed from Western Asia to other geographical regions. More population genetic structure studies involving various C. parvum subtype families using high-resolution tools are needed to better elucidate the origin and dissemination of C. parvum in the world.