1. NOVEL POTENTIAL PROBIOTICS FROM GUT MICROBIOTA OF HONEYBEES (APIS MELLIFERA) IN CLOVER FEEDING SEASON IN EGYPT.
- Author
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Khalafalla, Galal M., Sadik, Mahmoud W., Ali, Mahmoud A. M., and Mohamed, Rasha S.
- Subjects
PROBIOTICS ,GUT microbiome ,HONEYBEES ,PATHOGENIC microorganisms - Abstract
A total of 128 microbial isolates obtained from the gut of honey bees in different life stages in 2017 clover feeding season in Egypt was assessed for in vitro antagonistic activity against human pathogens. 52 out of 128 isolates have an antagonistic effect against one or more pathogens. 5 isolates out of 52 showed a high potential to act as probiotics. They were identified by molecular means as Wickerhamomyces anomalus, Lachancea thermotolerans, Zygosaccharomyces mellis, Bacillus licheniformis and Paenibacillus polymyxa. The antagonistic mechanisms were analyzed using cylinder diffusion assay and transmission electron microscopy. They tolerate pH up to 1.5 and bile salt conc. up to 3% after 3 hours of incubation. GC/MS analysis revealed the presence of Heptadecane, Palmitic acid, Dodemorph, Paraldehyde, Octadecnoic and Fenoprofen which may inhibit the growth of pathogenic microbes. Although it seems unconventional to use yeast strains and non-lactic acid bacteria as probiotics, these five isolates have a high potential to be used as probiotics for animals and humans. [ABSTRACT FROM AUTHOR]
- Published
- 2019