1. Comparative analysis of Shigella sonnei biotype g isolated from paediatric populations in Egypt, 1999-2005.
- Author
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Dejli J, Nada RA, Mansour A, El-Moniem AA, Wasfy MO, and Klena JD
- Subjects
- Bacterial Typing Techniques, Child, Preschool, DNA, Bacterial genetics, DNA, Bacterial metabolism, Disk Diffusion Antimicrobial Tests, Dysentery, Bacillary epidemiology, Dysentery, Bacillary microbiology, Egypt epidemiology, Electrophoresis, Gel, Pulsed-Field, Feces microbiology, Female, Humans, Infant, Male, Microbial Sensitivity Tests, Phylogeny, Plasmids genetics, Plasmids metabolism, Polymerase Chain Reaction, Prospective Studies, Restriction Mapping, Shigella sonnei classification, Shigella sonnei isolation & purification, Anti-Bacterial Agents pharmacology, DNA, Bacterial drug effects, Drug Resistance, Multiple, Bacterial, Plasmids drug effects, Shigella sonnei drug effects, Shigella sonnei genetics
- Abstract
Strain characteristics of 51 Shigella sonnei isolates obtained from children seeking medical care (MC) and 48 isolates recovered during a prospective diarrhoea birth cohort (BC) study were compared. Biochemical characterization and antibiotic susceptibility testing determined that all S. sonnei isolates were biotype g and multidrug-resistant. Plasmid profiling identified 15 closely related patterns and XbaI pulsed-field gel electrophoresis confirmed the high degree of genetic similarity between isolates. All S. sonnei isolates harboured ipaH and class II integrase genes and 84∙3 and 80% of the MC and BC isolates, respectively carried the sen gene. Neither the class I integrase nor the set gene was detected. Our results indicate that S. sonnei isolates associated with severe diarrhoea were indistinguishable from those associated with mild diarrhoea. Additional genetic tests with greater discrimination might offer an opportunity to determine genetic differences within the globally disseminating biotype g clone.
- Published
- 2013
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