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Aim: This Phase I study evaluated the safety and early efficacy of an aldosterone synthase inhibitor (BI 690517) in people with diabetes and albuminuric chronic kidney disease. Methods: Double‐blind, placebo‐controlled study (NCT03165240) at 40 sites across Europe. Eligible participants [estimated glomerular filtration rate ≥20 and <75 ml/min/1.73 m2; urine albumin/creatinine ratio (UACR) ≥200 and <3500 mg/g] were randomized 6:1 to receive once‐daily oral BI 690517 3, 10 or 40 mg, or eplerenone 25‐50 mg, or placebo, for 28 days. The primary endpoint was the proportion of participants with drug‐related adverse events (AEs). Secondary endpoints included changes from baseline in the UACR. Results: Fifty‐eight participants were randomized and treated from 27 November 2017 to 16 April 2020 (BI 690517: 3 mg, n = 18; 10 mg, n = 13; 40 mg, n = 14; eplerenone, n = 4; placebo, n = 9) for 28 days. Eight (13.8%) participants experienced drug‐related AEs [BI 690517: 3 mg (two of 18); 10 mg (four of 13); 40 mg (two of 14)], most frequently constipation [10 mg (one of 13); 40 mg (one of 14)] and hyperkalaemia [3 mg (one of 18); 10 mg (one of 13)]. Most AEs were mild to moderate; one participant experienced severe hyperkalaemia (serum potassium 6.9 mmol/L; BI 690517 10 mg). UACR responses [≥20% decrease from baseline (first morning void urine) after 28 days] were observed for 80.0% receiving BI 690517 40 mg (eight of 10) versus 37.5% receiving placebo (three of eight). Aldosterone levels were suppressed by BI 690517, but not eplerenone or placebo. Conclusions: BI 690517 was generally well tolerated, reduced plasma aldosterone and may decrease albuminuria in participants with diabetes and albuminuric chronic kidney disease. [ABSTRACT FROM AUTHOR]

Coastal dunes worldwide are increasingly under pressure from the adverse effects of human activities. Therefore, more and more restoration measures are being taken to create conditions that help disturbed coastal dune ecosystems regenerate or recover naturally. However, many projects lack the (open-access) monitoring observations needed to signal whether further actions are needed, and hence lack the opportunity to "learn by doing". This submission presents an open-access data set of 37 high-resolution digital elevation models and 24 orthomosaics collected before and after the excavation of five artificial foredune trough blowouts ("notches") in winter 2012/2013 in the Dutch Zuid-Kennemerland National Park, one of the largest coastal dune restoration projects in northwest Europe. These high-resolution data provide a valuable resource for improving understanding of the biogeomorphic processes that determine the evolution of restored dune systems as well as developing guidelines to better design future restoration efforts with foredune notching. Dataset:  https://doi.org/10.5281/zenodo.7010095 Dataset License: CC BY 4.0 [ABSTRACT FROM AUTHOR]

From the raw measurements at a single Global Navigation Satellite System (GNSS) ground-based station, the Zenith Total Delay (ZTD) and the tropospheric gradient can be estimated. In order to assimilate such data into Numerical Weather Prediction (NWP) models, the observation operator must be developed. Our previously developed tropospheric gradient operator is based on a linear combination of tropospheric delays and, therefore, is difficult to implement into NWP Data Assimilation (DA) systems. In this technical note, we develop a fast observation operator. This observation operator is based on an integral expression which contains the north–south and east–west horizontal gradients of refractivity. We run a numerical weather model (the horizontal resolution is 10 km) and show that for stations located in central Europe and in the warm season, the root-mean-square deviation between the tropospheric gradients calculated by the fast and original approach is about 0.15 mm. This deviation is regarded acceptable for assimilation since the typical root-mean-square deviation between observed and forward modelled tropospheric gradients is about 0.5 mm. We then implement the developed operator in our experimental DA system and test the proposed approach. In particular, we analyze the impact of the assimilation on the refractivity field. The developed tropospheric gradient operator, together with its tangent linear and adjoint version, is freely available (Fortran code) and ready to be implemented into NWP DA systems. [ABSTRACT FROM AUTHOR]

Introduction: Analgesia and sedation are essential for the care of children in the pediatric intensive care unit (PICU); however, when prolonged, they may be associated with iatrogenic withdrawal syndrome (IWS) and delirium. We sought to evaluate current practices on IWS and delirium assessment and management (including non‐pharmacologic strategies as early mobilization) and to investigate associations between the presence of an analgosedation protocol and IWS and delirium monitoring, analgosedation weaning, and early mobilization. Methods: We conducted a multicenter cross‐sectional survey‐based study collecting data from one experienced physician or nurse per PICU in Europe from January to April 2021. We then investigated differences among PICUs that did or did not follow an analgosedation protocol. Results: Among 357 PICUs, 215 (60%) responded across 27 countries. IWS was systematically monitored with a validated scale in 62% of PICUs, mostly using the Withdrawal Assessment Tool‐1 (53%). The main first‐line treatment for IWS was a rescue bolus with interruption of weaning (41%). Delirium was systematically monitored in 58% of PICUs, mostly with the Cornell Assessment of Pediatric Delirium scale (48%) and the Sophia Observation Scale for Pediatric Delirium (34%). The main reported first‐line treatment for delirium was dexmedetomidine (45%) or antipsychotic drugs (40%). Seventy‐one percent of PICUs reported to follow an analgosedation protocol. Multivariate analyses adjusted for PICU characteristics showed that PICUs using a protocol were significantly more likely to systematically monitor IWS (odds ratio [OR] 1.92, 95% confidence interval [CI] 1.01–3.67) and delirium (OR 2.00, 95% CI 1.07–3.72), use a protocol for analgosedation weaning (OR 6.38, 95% CI 3.20–12.71) and promote mobilization (OR 3.38, 95% CI 1.63–7.03). Conclusions: Monitoring and management of IWS and delirium are highly variable among European PICUs. The use of an analgosedation protocol was associated with an increased likelihood of monitoring IWS and delirium, performing a structured analgosedation weaning and promoting mobilization. Education on this topic and interprofessional collaborations are highly needed to help reduce the burden of analgosedation‐associated adverse outcomes. [ABSTRACT FROM AUTHOR]

The increasing impact of severe weather over Europe on lives and weather‐sensitive economies can be mitigated by accurate 0–6 h forecasts (nowcasts), supporting a vital 'last line of defence' for civil protection and many other applications. Recognizing lack of skill in some complex situations, often at convective and local sub‐kilometre scales and associated with rare events, we identify seven recommendations with the aim to improve nowcasting in Europe by the national meteorological and hydrological services (NMHSs) by 2030. These recommendations are based on a review of user needs, the state of the observing system, techniques based on observations and high‐resolution numerical weather models, as well as tools, data and infrastructure supporting the nowcasting community in Europe. Denser and more accurate observations are necessary particularly in the boundary layer to better characterize the ingredients of severe storms. A key driver for improvement is next‐generation European satellite data becoming available as of 2023. Seamless ensemble prediction methods to produce enhanced weather forecasts with 0–24 h lead times and probabilistic products require further development. Such products need to be understood and interpreted by skilled forecasters operating in an evolving forecasting context. We argue that stronger co‐development and collaboration between providers and users of nowcasting‐relevant data and information are key ingredients for progress. We recommend establishing pan‐European nowcasting consortia, better exchange of data, common development platforms and common verification approaches as key elements for progressing nowcasting in Europe in this decade. [ABSTRACT FROM AUTHOR]

OBJECTIVE: To analyze whether the coronavirus disease 2019 (COVID-19) pandemic increased the number of cases or impacted seasonality of new-onset type 1 diabetes (T1D) in large pediatric diabetes centers globally. RESEARCH DESIGN AND METHODS: We analyzed data on 17,280 cases of T1D diagnosed during 2018–2021 from 92 worldwide centers participating in the SWEET registry using hierarchic linear regression models. RESULTS: The average number of new-onset T1D cases per center adjusted for the total number of patients treated at the center per year and stratified by age-groups increased from 11.2 (95% CI 10.1–12.2) in 2018 to 21.7 (20.6–22.8) in 2021 for the youngest age-group, <6 years; from 13.1 (12.2–14.0) in 2018 to 26.7 (25.7–27.7) in 2021 for children ages 6 to <12 years; and from 12.2 (11.5–12.9) to 24.7 (24.0–25.5) for adolescents ages 12–18 years (all P < 0.001). These increases remained within the expected increase with the 95% CI of the regression line. However, in Europe and North America following the lockdown early in 2020, the typical seasonality of more cases during winter season was delayed, with a peak during the summer and autumn months. While the seasonal pattern in Europe returned to prepandemic times in 2021, this was not the case in North America. Compared with 2018–2019 (HbA1c 7.7%), higher average HbA1c levels (2020, 8.1%; 2021, 8.6%; P < 0.001) were present within the first year of T1D during the pandemic. CONCLUSIONS: The slope of the rise in pediatric new-onset T1D in SWEET centers remained unchanged during the COVID-19 pandemic, but a change in the seasonality at onset became apparent. [ABSTRACT FROM AUTHOR]

The introduction of non‐native species to new locations is a growing global phenomenon with major negative effects on native species and biodiversity. Such introductions potentially bring competitors into contact leading to partial or total species replacements. This creates an opportunity to study novel species interactions as they occur, with the potential to address the strength of inter‐ and intraspecific interactions, most notably competition. Such potential has often not been realized, however, due to the difficulties inherent in detecting rapid and spatially expansive species interactions under natural field conditions. The invasive amphipod crustacean Gammarus pulex has replaced a native species, Gammarus duebeni celticus, in river and lake systems across Europe. This replacement process is at least partially driven by differential parasitism, cannibalism, and intraguild predation, but the role of interspecific competition has yet to be resolved. Here, we examine how abundance of an invasive species may affect spatial niche breadth of a native congeneric species. We base our analyses of niche breadth on ordination and factor analysis of biological community and physical parameters, respectively, constituting a summative, multidimensional approach to niche breadth along environmental gradients. Results derived from biological and environmental niche criteria were consistent, although interspecific effects were stronger using the biological niche approach. We show that the niche breadth of the native species is constrained as abundance of the invader increases, but the converse effect does not occur. We conclude that the interaction between invasive G. pulex and native G. d. celticus under natural conditions is consistent with strong interspecific competition whereby a native, weaker competitor is replaced by a superior invasive competitor. This study indicates a strong role of interspecific competition, alongside other known interactions such as differential intraguild predation, in rapid and expansive species replacements following biological invasions. [ABSTRACT FROM AUTHOR]

This article examines organizational identification construction in mergers and acquisitions (M&As). The majority of existing studies have focused on antecedents and outcomes of post-merger identification (PMI) in Western contexts. While more and more Chinese cross-border M&As are taking place, how Chinese employees construct PMI remains underexplored. We adopted a qualitative case study approach to investigate how Chinese managers construct PMI after acquiring a European company. As the main contribution, we introduce the concept of agile organizational identity (AOI), wherein agility is a central, enduring and distinctive characteristic of an organization, i.e. 'who we are and who we want to be'. Our findings reveal that AOI is leveraged by Chinese managers to deal with their perceived inferior status, help them cope with the change and contribute to the construction of a strong PMI. We believe that our study provides a new perspective on how employees can effectively cope with organizational change while maintaining a sense of identity continuity. This article examines organizational identification construction in mergers and acquisitions (M&As). We provide novel insights by introducing the agile organizational identity (AOI) concept, wherein the agility is a central, enduring and distinctive characteristic of an organization, i.e. 'who we are and who we want to be'. To maintain such a pre-merger identity after M&As, people strive to continue changing as change is the continuity. Thus, psychological bonds between an employee and the employing organization do not become weaker. As agility is incorporated in the organizational identity, AOI helps employees to cope with the low status of their organization, accept organizational changes, and identify with the post-merger organization. Organizational leaders might want to foster AOI in order to successfully conduct strategic change initiatives. [ABSTRACT FROM AUTHOR]

Objectives: To obtain information from radiology departments throughout Europe regarding the practice of emergency radiology Methods: A survey which comprised of 24 questions was developed and made available online. The questionnaire was sent to 1097 chairs of radiology departments throughout Europe using the ESR database. All data were collected and analyzed using IBM SPSS Statistics software, version 20 (IBM). Results: A total of 1097 radiologists were asked to participate, 109 responded to our survey. The response rate was 10%. From our survey, 71.6% of the hospitals had more than 500 beds. Ninety-eight percent of hospitals have an active teaching affiliation. In large trauma centers, emergency radiology was considered a dedicated section. Fifty-three percent of institutions have dedicated emergency radiology sections. Less than 30% had all imaging modalities available. Seventy-nine percent of institutions have 24/7 coverage by staff radiologists. Emergency radiologists interpret cross-sectional body imaging, US scans, and basic CT/MRI neuroimaging in more than 50% of responding institutions. Cardiac imaging examinations/procedures are usually performed by cardiologist in 53% of institutions, while non-cardiac vascular procedures are largely performed and interpreted by interventional radiologists. Most people consider the European Diploma in Emergency Radiology an essential tool to advance the education and the dissemination of information within the specialty of emergency radiology. Conclusion: Emergency radiologists have an active role in the emergency medical team. Indeed, based upon our survey, they have to interact with emergency physicians and surgeons in the management of critically ill patients. A broad skillset from ultrasonography and basic neuroimaging is required. Key Points: • At most major trauma centers in Europe, emergency imaging is currently performed by all radiologists in specific units who are designated in the emergency department. • Radiologists in the emergency section at present have a broad skillset, which includes cross-sectional body imaging, ultrasonography, and basic neuroimaging of the brain and spine. • A dedicated curriculum that certifies a subspecialty in emergency radiology with a diploma offered by the European Society of Emergency Radiology demonstrates a great interest by the vast majority of the respondents. [ABSTRACT FROM AUTHOR]

Background: Growing international migration has increased the complexity of tuberculosis transmission patterns. Italy's decision to close its borders in 2018 made of Spain the new European porte entrée for migration from the Horn of Africa (HA). In one of the first rescues of migrants from this region at the end of 2018, tuberculosis was diagnosed in eight subjects, mainly unaccompanied minors.Methods: Mycobacterium tuberculosis isolates from these recently arrived migrants were analysed by Mycobacterial Interspersed Repetitive-Unit/Variable-Number of Tandem Repeat (MIRU-VNTR) and subsequent whole genome sequencing (WGS) analysis. Data were compared with those from collections from other European countries receiving migrants from the HA and a strain-specific PCR was applied for a fast searching of common strains. Infections in a cellular model were performed to assess strain virulence.Results: MIRU-VNTR analysis allowed identifying an epidemiological cluster involving three of the eight cases from Somalia (0 single-nucleotide polymorphisms between isolates, HA cluster). Following detailed interviews revealed that two of these cases had shared the same migratory route in most of the trip and had spent a long time at a detention camp in Libya. To confirm potential en route transmission for the three cases, we searched the same strain in collections from other European countries receiving migrants from the HA. MIRU-VNTR, WGS and a strain-specific PCR for the HA strain were applied. The same strain was identified in 12 cases from Eritrea diagnosed soon after their arrival in 2018 to the Netherlands, Belgium and Italy. Intracellular replication rate of the strain did not reveal abnormal virulence.Conclusions: Our study suggests a potential en route transmission of a pan-susceptible strain, which caused at least 15 tuberculosis cases in Somalian and Eritrean migrants diagnosed in four different European countries. [ABSTRACT FROM AUTHOR]

Around fifteen thousand fieldworkers annually count breeding birds using standardized protocols in 28 European countries. The observations are collected by using country-specific and standardized protocols, validated, summarized and finally used for the production of continent-wide annual and long-term indices of population size changes of 170 species. Here, we present the database and provide a detailed summary of the methodology used for fieldwork and calculation of the relative population size change estimates. We also provide a brief overview of how the data are used in research, conservation and policy. We believe this unique database, based on decades of bird monitoring alongside the comprehensive summary of its methodology, will facilitate and encourage further use of the Pan-European Common Bird Monitoring Scheme results. Measurement(s) population Technology Type(s) Count Factor Type(s) time scale • geographic location Sample Characteristic - Organism Aves Sample Characteristic - Location Europe Machine-accessible metadata file describing the reported data: https://doi.org/10.6084/m9.figshare.13240760 [ABSTRACT FROM AUTHOR]

Aim: The spread of invasive non‐native species (INNS) will pose major threats to global biodiversity over the coming decades. However, predicting how key effects of climate change will influence the abilities of INNS to establish and exert ecological impact is a major challenge. One overlooked aspect of global change is the expected freshening of certain marine systems, which may interact with INNS and lead to drastic effects on community structure and stability. Location: Baltic Sea, Europe. Methods: Here, using three predatory amphipod crustaceans, we experimentally assessed how salinity reduction may affect the impacts of the emerging INNS, Pontogammarus maeoticus, relative to an existing INNS, Gammarus tigrinus and a trophically analogous native, Gammarus salinus. We quantified per capita impacts of the three species via the comparative functional response method (prey consumption over a range of prey densities) under a predicted seawater freshening scenario. We then combined amphipod functional responses with their life history traits to compare population‐level relative impact potential (RIP) on prey of the three amphipod species across salinities. Results: Freshening substantially altered the predicted relative ecological impacts of both the INNS compared with the native. First, the functional responses of invasive P. maeoticus and G. tigrinus increased under freshening, while that of the native G. salinus decreased. Second, RIP became consistently higher for both the INNS compared to the native with increased freshening. Main conclusions: Our methods thus reveal potential for climate change via seawater freshening to drive large shifts in dominance and ecological impacts of INNS compared with natives. With the number of INNS introductions unlikely to saturate in the near future, we highlight the need to assess the impacts of potential future INNS, alongside established non‐natives and native species, in combination with abiotic changes associated with climate change. [ABSTRACT FROM AUTHOR]

Background: Although some national recommendations for the role of radiology in a polytrauma service exist, there are no European guidelines to date. Additionally, for many interdisciplinary guidelines, radiology tends to be under-represented. These factors motivated the European Society of Emergency Radiology (ESER) to develop radiologically-centred polytrauma guidelines. Results: Evidence-based decisions were made on 68 individual aspects of polytrauma imaging at two ESER consensus conferences. For severely injured patients, whole-body CT (WBCT) has been shown to significantly reduce mortality when compared to targeted, selective CT. However, this advantage must be balanced against the radiation risk of performing more WBCTs, especially in less severely injured patients. For this reason, we recommend a second lower dose WBCT protocol as an alternative in certain clinical scenarios. The ESER Guideline on Radiological Polytrauma Imaging and Service is published in two versions: a full version (download from the ESER homepage, https://www.eser-society.org) and a short version also covering all recommendations (this article). Conclusions: Once a patient has been accurately classified as polytrauma, each institution should be able to choose from at least two WBCT protocols. One protocol should be optimised regarding time and precision, and is already used by most institutions (variant A). The second protocol should be dose reduced and used for clinically stable and oriented patients who nonetheless require a CT because the history suggests possible serious injury (variant B). Reading, interpretation and communication of the report should be structured clinically following the ABCDE format, i.e. diagnose first what kills first. [ABSTRACT FROM AUTHOR]

Prioritizing the management of invasive alien species (IAS) is of global importance and within Europe integral to the EU IAS regulation. To prioritize management effectively, the risks posed by IAS need to be assessed, but so too does the feasibility of their management. While the risk of IAS to the EU has been assessed, the feasibility of management has not. We assessed the feasibility of eradicating 60 new (not yet established) and 35 emerging (established with limited distribution) species that pose a threat to the EU, as identified by horizon scanning. The assessment was carried out by 34 experts in invasion management from across Europe, applying the Non‐Native Risk Management scheme to defined invasion scenarios and eradication strategies for each species, assessing the feasibility of eradication using seven key risk management criteria. Management priorities were identified by combining scores for risk (derived from horizon scanning) and feasibility of eradication. The results show eradication feasibility score and risk score were not correlated, indicating that risk management criteria evaluate different information than risk assessment. In all, 17 new species were identified as particularly high priorities for eradication should they establish in the future, whereas 14 emerging species were identified as priorities for eradication now. A number of species considered highest priority for eradication were terrestrial vertebrates, a group that has been the focus of a number of eradication attempts in Europe. However, eradication priorities also included a diverse range of other taxa (plants, invertebrates and fish) suggesting there is scope to broaden the taxonomic range of attempted eradication in Europe. We demonstrate that broad scale structured assessments of management feasibility can help prioritize IAS for management. Such frameworks are needed to support evidence‐based decision‐making. [ABSTRACT FROM AUTHOR]

We describe a procedure to quantify emissions of chemicals for environmental protection, assessment, and management purposes. The procedure uses production and use volumes from registration dossiers and combines these with Specific Environmental Release Category data. The procedure was applied in a case study. Emission estimations were made for chemicals registered under the European Union chemicals regulations for industrial chemicals (Registration, Evaluation, Authorisation and Restriction of Chemicals [REACH]) and for the active ingredients of medicines and crop protection products. Emissions themselves cannot be validated. Instead, emission estimates were followed by multimedia fate modeling and mixture toxic pressure modeling to arrive at predicted environmental concentrations (PECs) and toxic pressures for a typical European water body at steady state, which were compared with other such data. The results show that screening‐level assessments could be performed, and yielded estimates of emissions, PECs, and mixture toxic pressures of chemicals used in Europe. Steady‐state PECs agreed fairly well with measured concentrations. The mixture toxic pressure at steady state suggests the presence of effects in aquatic species assemblages, whereby few compounds dominate the predicted impact. The study shows that our screening‐level emission estimation procedure is sufficiently accurate and precise to serve as a basis for assessment of chemical pollution in aquatic ecosystems at the scale of river catchments. Given a recognized societal need to develop methods for realistic, cumulative exposures, the emission assessment procedure can assist in the prioritization of chemicals in safety policies (such as the European Union REACH regulation), where "possibility to be used safely" needs to be demonstrated, and environmental quality policies (such as the European Union Water Framework Directive), where "good environmental quality" needs to be reached. Environ Toxicol Chem 2020;39:1839–1851. © 2020 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC. [ABSTRACT FROM AUTHOR]

To describe current practices in European Paediatric Intensive Care Units (PICUs) regarding neuro-prognostication in comatose children after cardiac arrest (CA). An anonymous online survey was conducted among members of the European Society of Paediatric and Neonatal Intensive Care (ESPNIC) and the European Paediatric Neurology Society (EPNS) throughout January and February 2019. The survey consisted of 49 questions divided into 4 sections: general information, cardiac arrest, neuro-prognostication and follow-up. The survey was sent to 1310 EPNS and 611 ESPNIC members. Of the 108 respondents, 71 (66%) (23 countries, 45 PICUs) completed the "neuro-prognostication" section. Eight PICUs (20%) had a local neuro-prognostication guideline. The 3 methods considered as most useful were neurological examination (92%), magnetic resonance imaging (MRI) (82%) and continuous electroencephalography (cEEG) (45%). In 50% a Pediatric Cerebral Performance Category (PCPC) score ≥ 4 was considered as poor neurological outcome. In 63% timing of determining neurological prognosis was based on the individual patient. Once decided that neurological prognosis was futile, 55% indicated that withdrawing life-sustaining therapy (WLST) was (one of) the options, whereas 44% continued PICU treatment (with or without restrictions). In 28 PICUs (68%) CA-survivors were scheduled for follow-up visits. Local guidelines for neuro-prognostication in comatose children after CA are uncommon. Methods to assess neurological outcome were mainly neurological examination, MRI and cEEG. Consequences of poor outcome differed between respondents. Inaccuracies in neuro-prognostication can result in premature WLST, thereby biasing outcome research and creating a self-fulfilling cycle. Further research is needed to develop scientifically based international guidelines for neuro-prognostication in comatose children after CA. • Neuro-prognostication guidelines in comatose cardiac arrest survivors are uncommon. • Neurologic exam, MRI and cEEG are considered most useful in order to prognosticate. • Once decided the prognosis is futile, treatment consequences differed across Europe. [ABSTRACT FROM AUTHOR]

High‐throughput high‐density genotyping arrays continue to be a fast, accurate, and cost‐effective method for genotyping thousands of polymorphisms in high numbers of individuals. Here, we have developed a new high‐density SNP genotyping array (103,270 SNPs) for honey bees, one of the most ecologically and economically important pollinators worldwide. SNPs were detected by conducting whole‐genome resequencing of 61 honey bee drones (haploid males) from throughout Europe. Selection of SNPs for the chip was done in multiple steps using several criteria. The majority of SNPs were selected based on their location within known candidate regions or genes underlying a range of honey bee traits, including hygienic behavior against pathogens, foraging, and subspecies. Additionally, markers from a GWAS of hygienic behavior against the major honey bee parasite Varroa destructor were brought over. The chip also includes SNPs associated with each of three major breeding objectives—honey yield, gentleness, and Varroa resistance. We validated the chip and make recommendations for its use by determining error rates in repeat genotypings, examining the genotyping performance of different tissues, and by testing how well different sample types represent the queen's genotype. The latter is a key test because it is highly beneficial to be able to determine the queen's genotype by nonlethal means. The array is now publicly available and we suggest it will be a useful tool in genomic selection and honey bee breeding, as well as for GWAS of different traits, and for population genomic, adaptation, and conservation questions. [ABSTRACT FROM AUTHOR]

Central Europe has a vital and extensive meteorological research community comprising national weather services, universities, and research organizations and institutes. Nearly all of them are involved in the open scientific questions regarding clouds and precipitation processes. The research activities include observations (from in situ ground-based remote sensing radio soundings to satellite-based observations), model development on all scales (from direct numerical simulations to global climate models), and other activities. With Germany as an example our first objective is to show the large amount and the diversity of observations regarding clouds and precipitation. The goal is to give an overview of existing measurements and datasets to show the benefit of combining the different information from a variety of observations. Up to now the access to and the usage of these datasets from different sources was not straightforward, due to the issue of missing data and archiving standards for observational data. This then motivates our second objective, which is to introduce our solution for this issue—the novel Standardized Atmospheric Measurement Data archive (SAMD). SAMD is one of the outcomes of the German research initiative High Definition Clouds and Precipitation for Advancing Climate Prediction [HD(CP)2]. The goal of SAMD is an easy-to-use approach for both data producers and archive users. Therefore the archive provides observational data in the common Climate Forecast (CF) Conventions format and makes it available to the broader public. SAMD offers highly standardized quality-controlled data and metadata for a wide range of instruments, with open access, which makes this novel archive important for the research community. [ABSTRACT FROM AUTHOR]

An analysis of processing settings impacts on estimated tropospheric gradients is presented. The study is based on the benchmark data set collected within the COST GNSS4SWEC action with observations from 430 Global Navigation Satellite Systems (GNSS) reference stations in central Europe for May and June 2013. Tropospheric gradients were estimated in eight different variants of GNSS data processing using precise point positioning (PPP) with the G-Nut/Tefnut software. The impacts of the gradient mapping function, elevation cut-off angle, GNSS constellation, observation elevation-dependent weighting and real-time versus post-processing mode were assessed by comparing the variants by each to other and by evaluating them with respect to tropospheric gradients derived from two numerical weather models (NWMs). Tropospheric gradients estimated in post-processing GNSS solutions using final products were in good agreement with NWM outputs. The quality of high-resolution gradients estimated in (near-)real-time PPP analysis still remains a challenging task due to the quality of the real-time orbit and clock corrections. Comparisons of GNSS and NWM gradients suggest the 3 ∘ elevation angle cut-off and GPS + GLONASS constellation for obtaining optimal gradient estimates provided precise models for antenna-phase centre offsets and variations, and tropospheric mapping functions are applied for low-elevation observations. Finally, systematic errors can affect the gradient components solely due to the use of different gradient mapping functions, and still depending on observation elevation-dependent weighting. A latitudinal tilting of the troposphere in a global scale causes a systematic difference of up to 0.3 mm in the north-gradient component, while large local gradients, usually pointing in a direction of increasing humidity, can cause differences of up to 1.0 mm (or even more in extreme cases) in any component depending on the actual direction of the gradient. Although the Bar-Sever gradient mapping function provided slightly better results in some aspects, it is not possible to give any strong recommendation on the gradient mapping function selection. [ABSTRACT FROM AUTHOR]

Objectives: Previous studies in food-producing animals have shown associations between antimicrobial use (AMU) and resistance (AMR) in specifically isolated bacterial species. Multi-country data are scarce and only describe between-country differences. Here we investigate associations between the pig faecal mobile resistome and characteristics at the farm-level across Europe.Methods: A cross-sectional study was conducted among 176 conventional pig farms from nine European countries. Twenty-five faecal samples from fattening pigs were pooled per farm and acquired resistomes were determined using shotgun metagenomics and the Resfinder reference database, i.e. the full collection of horizontally acquired AMR genes (ARGs). Normalized fragments resistance genes per kilobase reference per million bacterial fragments (FPKM) were calculated. Specific farm-level data (AMU, biosecurity) were collected. Random-effects meta-analyses were performed by country, relating farm-level data to relative ARG abundances (FPKM).Results: Total AMU during fattening was positively associated with total ARG (total FPKM). Positive associations were particularly observed between widely used macrolides and tetracyclines, and ARGs corresponding to the respective antimicrobial classes. Significant AMU-ARG associations were not found for β-lactams and only few colistin ARGs were found, despite high use of these antimicrobial classes in younger pigs. Increased internal biosecurity was directly related to higher abundances of ARGs mainly encoding macrolide resistance. These effects of biosecurity were independent of AMU in mutually adjusted models.Conclusions: Using resistome data in association studies is unprecedented and adds accuracy and new insights to previously observed AMU-AMR associations. Major components of the pig resistome are positively and independently associated with on-farm AMU and biosecurity conditions. [ABSTRACT FROM AUTHOR]

Background: Previous studies indicate that low initial sensitivity to alcohol may be a risk factor for later alcohol misuse. Evidence suggests that initial sensitivity is influenced by genetic factors, but few molecular genetic studies have been reported. Methods: We conducted a meta‐analysis of 2 population‐based genome‐wide association studies of the Self‐Rating of the Effects of Alcohol scale. Our final sample consisted of 7,339 individuals (82.3% of European descent; 59.2% female) who reported having used alcohol at least 5 times. In addition, we estimated single nucleotide polymorphism (SNP)‐based heritability and conducted a series of secondary aggregate genetic analyses. Results: No individual locus reached genome‐wide significance. Gene and set based analyses, both overall and using tissue‐specific expression data, yielded largely null results, and genes previously implicated in alcohol problems and consumption were overall not associated with initial sensitivity. Only 1 gene set, related to hormone signaling and including core clock genes, survived correction for multiple testing. A meta‐analysis of SNP‐based heritability resulted in a modest estimate of hSNP2 = 0.19 (SE = 0.10), though this was driven by 1 sample (N = 3,683, hSNP2 = 0.36, SE = 0.14, p = 0.04). No significant genetic correlations with other relevant outcomes were observed. Conclusions: Findings yielded only modest support for a genetic component underlying initial alcohol sensitivity. Results suggest that its biological underpinnings may diverge somewhat from that of other alcohol outcomes and may be related to core clock genes or other aspects of hormone signaling. Larger samples, ideally of prospectively assessed samples, are likely necessary to improve gene identification efforts and confirm the current findings. Initial sensitivity to alcohol is associated with later problematic alcohol‐related outcomes. The current study found evidence of aggregate genetic influences on initial sensitivity, though no individual SNP met genome‐wide significance criteria. Further analyses suggest that the biology of initial sensitivity may diverge from that of other alcohol outcomes. As with other complex traits, how initial sensitivity is assessed is likely critical to the identification of robust genetic signals. [ABSTRACT FROM AUTHOR]

Background/Aims: The prevalence of diabetes mellitus type 2 and kidney disease in these patients varies widely between European countries. Methods: In addition to store bio-samples the “Prospective cohort study in patients with type 2 diabetes mellitus for validation of biomarkers” collects information on history, physical status, laboratory measurements and medication in 4000 patients with diabetes mellitus type 2, being taken care of at the primary level of healthcare in 5 European countries (Austria, Hungary, Netherlands, Poland and Scotland). Next to comparing the rate of loss of eGFR between the countries, a further objective of the PROVALID study is to determine the 5-year cumulative incidence of renal and cardiovascular outcomes. Results: The mean age of the population recruited is 62.9±10 years, 54.6% are male and the mean BMI is 30.9±5.4 kg/m2. Metabolic control (median HBA1c 6.8 % (6.2; 7.5)) is achieved via administration of metformin in 67.4% of the patients and insulin in 30.3%. Median systolic and diastolic blood pressure at recruitment is 135 (125; 146) and 80 (72; 85) mmHg, 65.4% of subjects received RAAS blocking agents. Mean eGFR is 80.7±29.2 ml/min/1.73m2 and median baseline albumin/creatinine ratio 8.3 mg (IQR: 3.8 and 25.1). Conclusion: PROVALID will provide information on incidence and progression of renal and cardiovascular disease and therapy in patients with type 2 diabetes mellitus in different European countries. Thus, in contrast to many other cohort studies we will be able to associate national clinical practise pattern with outcome in this highly vulnerable patient population. [ABSTRACT FROM AUTHOR]

The market for new durable products of modified wood has increased substantially during the last few years, especially in Europe. This increased interest depends partly on the restricted use of toxic preservatives due to increased environmental concern, as well as the need for reduced maintenance for wood products that are mainly for exterior use. Furthermore, as sustainability becomes a greater concern, the environmental impact of construction and interior materials should be included in planning by considering the entire life cycle and embodied energy of the materials used. As a result, wood modification has been implemented to improve the intrinsic properties of wood, widen the range of sawn timber applications, and acquire the form and functionality desired by engineers, without bringing environmental friendliness into question. The different wood modification processes are at various stages of development, and the challenges that must be overcome to expand to industrial applications differ amongst them. In this paper, three groups of wood modification processes are discussed and exemplified with modified wood products that have been newly introduced to the market: (i) chemical processing (acetylation, furfurylation, resin impregnation etc.); (ii) thermo-hydro processing (thermal treatment); and (iii) thermo-hydro-mechanical processing (surface densification). Building on these examples, the paper will discuss the environmental impact assessment of modification processes and further development needs. [ABSTRACT FROM AUTHOR]

Wood is a raw material with multifaceted properties making it useful for a diversity of industrial products. However, its primary production is restricted by environmental conditions and alternative land use. Forestry, as the main supplier of wood as raw material, is assumed to act strategically with regard to the market situation and to try, therefore, to provide the most beneficial mix of different assortments of wood. Changes in the supply of different assortments and their current value on the market are assumed to be closely related. Fluctuations in stock and market value affect the wood-using industry depending on the different assortments as raw material. The purpose of this study was to apply a concept of competitive strategies for market-driven goods to forest management in Europe, in order to derive information about the operational possibilities of forest management in specific market situations. For this purpose, Steinmann's and Schreyögg's concept of competitive strategies for market-driven goods was applied to forest management, in order to defining different competitive strategies for the production of different assortments of wood. The results show that different market strategies can be applied to forest management and that, even though the overall production is limited, forestry has quite a high flexibility regarding the competitive strategy used. The results also show possible impacts on the nature of forests due to changes in forest management. [ABSTRACT FROM AUTHOR]

The literature about secularization proposes two distinct explanations of anti-Muslim sentiment in secularized societies. The first theory understands it in terms of religious competition between Muslims and the remaining minority of orthodox Protestants; the second understands it as resulting from value conflicts between Muslims and the nonreligious majority. The two theories are tested by means of a multilevel analysis of the European Values Study 2008. Our findings indicate that, although more secularized countries are on average more tolerant towards Muslims and Islam, strongest anti-Muslim attitudes are nonetheless found among the nonreligious in these countries. [ABSTRACT FROM AUTHOR]

Introduction: Previous studies in adolescents were not adequately powered to accurately disentangle genetic and environmental influences on smoking initiation (SI) across adolescence.Methods: Mega-analysis of pooled genetically informative data on SI was performed, with structural equation modeling, to test equality of prevalence and correlations across cultural backgrounds, and to estimate the significance and effect size of genetic and environmental effects according to the classical twin study, in adolescent male and female twins from same-sex and opposite-sex twin pairs (N = 19 313 pairs) between ages 10 and 19, with 76 358 longitudinal assessments between 1983 and 2007, from 11 population-based twin samples from the United States, Europe, and Australia.Results: Although prevalences differed between samples, twin correlations did not, suggesting similar etiology of SI across developed countries. The estimate of additive genetic contributions to liability of SI increased from approximately 15% to 45% from ages 13 to 19. Correspondingly, shared environmental factors accounted for a substantial proportion of variance in liability to SI at age 13 (70%) and gradually less by age 19 (40%).Conclusions: Both additive genetic and shared environmental factors significantly contribute to variance in SI throughout adolescence. The present study, the largest genetic epidemiological study on SI to date, found consistent results across 11 studies for the etiology of SI. Environmental factors, especially those shared by siblings in a family, primarily influence SI variance in early adolescence, while an increasing role of genetic factors is seen at later ages, which has important implications for prevention strategies.Implications: This is the first study to find evidence of genetic factors in liability to SI at ages as young as 12. It also shows the strongest evidence to date for decay of effects of the shared environment from early adolescence to young adulthood. We found remarkable consistency of twin correlations across studies reflecting similar etiology of liability to initiate smoking across different cultures and time periods. Thus familial factors strongly contribute to individual differences in who starts to smoke with a gradual increase in the impact of genetic factors and a corresponding decrease in that of the shared environment. [ABSTRACT FROM AUTHOR]

Aim In the alpine life zone, plant diversity is strongly determined by local topography and microclimate. We assessed the extent to which aspect and its relatedness to temperature affect plant species diversity, and the colonization and disappearance of species on alpine summits on a pan-European scale. Location Mountain summits in Europe's alpine life zone. Methods Vascular plant species and their percentage cover were recorded in permanent plots in each cardinal direction on 123 summits in 32 regions across Europe. For a subset from 17 regions, resurvey data and 6-year soil temperature series were available. Differences in temperature sum and Shannon index as well as species richness, colonization and disappearance of species among cardinal directions were analysed using linear mixed-effects and generalised mixed-effects models, respectively. Results Temperature sums were higher in east- and south-facing aspects than in the north-facing ones, while the west-facing ones were intermediate; differences were smallest in northern Europe. The patterns of temperature sums among aspects were consistent among years. In temperate regions, thermal differences were reflected by plant diversity, whereas this relationship was weaker or absent on Mediterranean and boreal mountains. Colonization of species was positively related to temperature on Mediterranean and temperate mountains, whereas disappearance of species was not related to temperature. Main conclusions Thermal differences caused by solar radiation determine plant species diversity on temperate mountains. Advantages for plants on eastern slopes may result from the combined effects of a longer diurnal period of radiation due to convection cloud effects in the afternoon and the sheltered position against the prevailing westerly winds. In northern Europe, long summer days and low sun angles can even out differences among aspects. On Mediterranean summits, summer drought may limit species numbers on the warmer slopes. Warmer aspects support a higher number of colonization events. Hence, aspect can be a principal determinant of the pace of climate-induced migration processes. [ABSTRACT FROM AUTHOR]

Background: This position statement provides clinical recommendations for the assessment of pain, level of sedation, iatrogenic withdrawal syndrome and delirium in critically ill infants and children. Admission to a neonatal or paediatric intensive care unit (NICU, PICU) exposes a child to a series of painful and stressful events. Accurate assessment of the presence of pain and non-pain-related distress (adequacy of sedation, iatrogenic withdrawal syndrome and delirium) is essential to good clinical management and to monitoring the effectiveness of interventions to relieve or prevent pain and distress in the individual patient.Methods: A multidisciplinary group of experts was recruited from the members of the European Society of Paediatric and Neonatal Intensive Care (ESPNIC). The group formulated clinical questions regarding assessment of pain and non-pain-related distress in critically ill and nonverbal children, and searched the PubMed/Medline, CINAHL and Embase databases for studies describing the psychometric properties of assessment instruments. Furthermore, level of evidence of selected studies was assigned and recommendations were formulated, and grade or recommendations were added on the basis of the level of evidence.Results: An ESPNIC position statement was drafted which provides clinical recommendations on assessment of pain (n = 5), distress and/or level of sedation (n = 4), iatrogenic withdrawal syndrome (n = 3) and delirium (n = 3). These recommendations were based on the available evidence and consensus amongst the experts and other members of ESPNIC.Conclusions: This multidisciplinary ESPNIC position statement guides professionals in the assessment and reassessment of the effectiveness of treatment interventions for pain, distress, inadequate sedation, withdrawal syndrome and delirium. [ABSTRACT FROM AUTHOR]

Global Navigation Satellite Systems (GNSS) have revolutionised positioning, navigation, and timing, becoming a common part of our everyday life. Aside from these well-known civilian and commercial applications, GNSS is now an established atmospheric observing system, which can accurately sense water vapour, the most abundant greenhouse gas, accounting for 60-70 % of atmospheric warming. In Europe, the application of GNSS in meteorology started roughly two decades ago and today it is a well-established research field. This review covers the state-of-the-art in GNSS meteorology in Europe. Discussed are the advances in GNSS processing for derivation of tropospheric products, application of GNSS tropospheric products in operational weather prediction and application of GNSS tropospheric products for climate monitoring. Reviewed are the GNSS processing techniques and tropospheric products. Given is a summary of the use of the products for validation and impact studies with operational Numerical Weather Prediction (NWP) models as well as very short weather prediction (nowcasting) case studies. Climate research with GNSS is an emerging field of research, the studies so far have been limited to comparison with the climate models and derivation of trends. More than 15 years of GNSS meteorology in Europe has already achieved outstanding cooperation between the atmospheric and geodetic communities. It is now feasible to develop next-generation GNSS tropospheric products and applications that can enhance the quality of weather forecasts and climate monitoring. This work is carried out within COST Action ES1206 "Advanced Global Navigation Satellite Systems tropospheric products for monitoring Severe Weather Events and Climate" (GNSS4SWEC, http://gnss4swec.knmi.nl ). [ABSTRACT FROM AUTHOR]

To evaluate progress on political biodiversity objectives, biodiversity monitoring provides information on whether intended results are being achieved. Despite scientific proof that monitoring and evaluation increase the (cost) efficiency of policy measures, cost estimates for monitoring schemes are seldom available, hampering their inclusion in policy programme budgets., Empirical data collected from 12 case studies across Europe were used in a power analysis to estimate the number of farms that would need to be sampled per major farm type to detect changes in species richness over time for four taxa (vascular plants, earthworms, spiders and bees). A sampling design was developed to allocate spatially, across Europe, the farms that should be sampled., Cost estimates are provided for nine monitoring scenarios with differing robustness for detecting temporal changes in species numbers. These cost estimates are compared with the Common Agricultural Policy ( CAP) budget (2014-2020) to determine the budget allocation required for the proposed farmland biodiversity monitoring., Results show that the bee indicator requires the highest number of farms to be sampled and the vascular plant indicator the lowest. The costs for the nine farmland biodiversity monitoring scenarios corresponded to 0·01%-0·74% of the total CAP budget and to 0·04%-2·48% of the CAP budget specifically allocated to environmental targets., Synthesis and applications. The results of the cost scenarios demonstrate that, based on the taxa and methods used in this study, a Europe-wide farmland biodiversity monitoring scheme would require a modest share of the Common Agricultural Policy budget. The monitoring scenarios are flexible and can be adapted or complemented with alternate data collection options (e.g. at national scale or voluntary efforts), data mobilization, data integration or modelling efforts. [ABSTRACT FROM AUTHOR]

Invasive alien aquatic species, including marine and freshwater macroinvertebrates, have become increasingly important in terms of both environmental and socio-economic impacts. In order to assess their environmental and economic costs, we applied the Generic Impact Scoring System (GISS) and performed a comparison with other taxa of invaders in Europe. Impacts were scored into six environmental and six socioeconomic categories, with each category containing five impact levels. Among 49 aquatic macroinvertebrates, the most impacting species were the Chinese mitten crab, Eriocheir sinensis (Milne-Edwards, 1853) and the zebra mussel, Dreissena polymorpha (Pallas, 1771). The highest impacts found per GISS impact category were, separately; on ecosystems, through predation, as competitors, and on animal production. Eleven species have an impact score > 10 (high impact) and seven reach impact level 5 in at least one impact category (EU blacklist candidates), the maximum score that can be given is 60 impact points. Comparisons were drawn between aquatic macroinvertebrates and vertebrate invaders such as fish, mammals and birds, as well as terrestrial arthropods, revealing invasive freshwater macroinvertebrates to be voracious predators of native prey and damaging to native ecosystems compared with other taxa. GISS can be used to compare these taxa and will aid policy making and targeting of invasive species for management by relevant agencies, or to assist in producing species blacklist candidates. [ABSTRACT FROM AUTHOR]

The political situation in the Soviet Union during the twentieth century has led some to suggest that socialism is some kind of secular religion as opposed to ‘normal’ religion. In modern Europe, however, there have been vibrant Christian socialist movements. This article looks into the different attitudes of socialists towards religion and answers the question whether it is pressure of religious activity or pressure of religious identity that makes socialists resist religion. The results from a multilevel analysis of three waves of the European Values Study (1990–2008) in 21 Western European countries specifically point to an increase in anti-religiosity by socialists in countries marked by Catholic and Orthodox religious identities. [ABSTRACT FROM PUBLISHER]

Studying the evolutionary history of trait divergence, in particular those related to dispersal capacity, is of major interest for the process of local adaptation and metapopulation dynamics. Here, we reconstruct the evolution of different alleles at the nuclear-encoded mitochondrial NADP+-dependent isocitrate dehydrogenase (mtIdh) locus of the ground beetle Pogonus chalceus that are differentially and repeatedly selected in short- and long-winged populations in response to different hydrological regimes at both allopatric and sympatric scales along the Atlantic European coasts. We sequenced 2788 bp of the mtIdh locus spanning a ~7-kb genome region and compared its variation with that of two supposedly neutral genes. mtIdh sequences show (i) monophyletic clustering of the short-winged associated mtIDH-DE haplotypes within the long-winged associated mtIDH-AB haplotypes, (ii) a more than tenfold lower haplotype diversity associated with the mtIDH-DE alleles compared to the mtIDH-AB alleles and (iii) a high number of fixed nucleotide differences between both mtIDH haplotype clusters. Coalescent simulations suggest that this observed sequence variation in the mtIdh locus is most consistent with a singular origin in a partially isolated subpopulation, followed by a relatively recent spread of the mtIDHDE allele in short-winged populations along the Atlantic coast. These results demonstrate that even traits associated with decreased dispersal capacity can rapidly spread and that reuse of adaptive alleles plays an important role in the adaptive potential within this sympatric mosaic of P. chalceus populations. [ABSTRACT FROM AUTHOR]

Profiles heavy buyers of store brand products and compares them with light buyers in terms of demographics, socio-economic, and attitudinal variables. The results suggest that younger, unmarried, and smaller sized households tend to avoid store brands. As compared with heavy buyers, light buyers of store brands are less familiar with them and perceive them to be of lower quality, less value for money and as riskier choices. [ABSTRACT FROM AUTHOR]

Mapping of environmental variables often relies on map accuracy assessment through cross-validation with the data used for calibrating the underlying mapping model. When the data points are spatially clustered, conventional cross-validation leads to optimistically biased estimates of map accuracy. Several papers have promoted spatial cross-validation as a means to tackle this over-optimism. Many of these papers blame spatial autocorrelation as the cause of the bias and propagate the widespread misconception that spatial proximity of calibration points to validation points invalidates classical statistical validation of maps. We present and evaluate alternative cross-validation approaches for assessing map accuracy from clustered sample data. The first method uses inverse sampling-intensity weighting to correct for selection bias. Sampling-intensity is estimated by a two-dimensional kernel approach. The two other approaches are model-based methods rooted in geostatistics, where the first assumes homogeneity of residual variance over the study area whilst the second accounts for heteroscedasticity as a function of the sampling intensity. The methods were tested and compared against conventional k -fold cross-validation and blocked spatial cross-validation to estimate map accuracy metrics of above-ground biomass and soil organic carbon stock maps covering western Europe. Results acquired over 100 realizations of five sampling designs ranging from non-clustered to strongly clustered confirmed that inverse sampling-intensity weighting and the heteroscedastic model-based method had smaller bias than conventional and spatial cross-validation for all but the most strongly clustered design. For the strongly clustered design where large portions of the maps were predicted by extrapolation, blocked spatial cross-validation was closest to the reference map accuracy metrics, but still biased. For such cases, extrapolation is best avoided by additional sampling or limitation of the prediction area. Weighted cross-validation is recommended for moderately clustered samples, while conventional random cross-validation suits fairly regularly spread samples. • Cross-validation with clustered data produces too optimistic map accuracy estimates. • In contrast, blocked spatial cross-validation is pessimistically biased. • Sampling-intensity weighted cross-validation is recommended for clustered samples. [ABSTRACT FROM AUTHOR]