1. Characteristics of hepatitis B virus genotype G coinfected with genotype H in chimeric mice carrying human hepatocytes.
- Author
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Tanaka Y, Sanchez LV, Sugiyama M, Sakamoto T, Kurbanov F, Tatematsu K, Roman S, Takahashi S, Shirai T, Panduro A, and Mizokami M
- Subjects
- Animals, Base Sequence, Cell Transplantation, Cells, Cultured, Chimera, Europe, Female, Hepatitis B pathology, Hepatitis B virus classification, Hepatocytes transplantation, Hepatocytes virology, Humans, Liver Cirrhosis pathology, Liver Cirrhosis virology, Male, Mexico, Mice, Mice, SCID, Molecular Sequence Data, Sequence Alignment, Genome, Viral, Hepatitis B virology, Hepatitis B virus physiology, Virus Replication
- Abstract
Accumulated evidence indicated that hepatitis B virus genotype G (HBV/G) is present exclusively in coinfection with other HBV genotypes. In Mexico, HBV/G from 6 men who had sex with men were coinfected with HBV/H. Phylogenetically complete genomes of the 6 Mexican HBV/G strains were closely related to previous ones from the US/Europe. Using uPA/SCID mice with human hepatocytes, monoinfection with HBV/G did not result in detectable HBV DNA in serum, whereas superinfection with HBV/G at week 10 inoculated HBV/H when HBV/H DNA was elevated to >10(7) copies/mL has enhanced the replication of HBV/G. The HBV/G was enhanced in another 3 inoculated with a serum passage containing HBV/G with a trace of HBV/H. Coinfection of mice with HBV/G and H induced fibrosis in the liver. In conclusion, the replication of HBV/G can be enhanced remarkably when it is coinfected with HBV/H. Coinfection with HBV/G may be directly cytopathic in immunosuppressive conditions.
- Published
- 2008
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