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This article focuses on a study related to some biological species as of January 1998. To determine the phylogenetic positions of two new species, armillaria jezoensis and aimillaria singula and one new subspecies, the nucleotide sequences of the intergenic spacers of their ribosomal DNA were investigated, and compared with those of four other armillaria species from Japan and those of nine armillaria species from Europe and North America. The authors conclude that Armillaria jezoensis, and armillaria singula belong to the armillaria gallica cluster as armillaria cepistipes, armillaria gallica and armillaria sinapina from Japan.