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One new 14-membered ring cyclopeptide alkaloid, plenane A (1), along with six known compounds (2-7), was isolated from the roots of Clematis florida. Their structures were elucidated by means of NMR spectroscopic analysis and mass spectrometry. In addition, their anti- inflammatory effects on lipopolysaccharide-induced RAW264.7 macrophages were evaluated in vitro. The compounds 1, 2 and 6 exhibited potent indirect inhibitory effects, with IC50 values of 40.92, 22.88 and 6.32 μM, respectively. [ABSTRACT FROM AUTHOR]

One new indole-type alkaloid, α -L-rhamnopyranosyl-(1→6)- β -D- glucopyranosyl 6-methoxy-3-indolecarbonate ( 1 ), together with three known alkaloids ( 2 - 4 ), one aromatic acid ( 5 ) and five known saponins ( 6 - 10 ), was isolated from the roots of Clematis florida var. plena . Their structures were established by NMR spectroscopic analysis and acid hydrolysis. In in vivo anti-inflammatory activity, n -butanol extract was found to be potent against ear edema in mice, with inhibition rate of 48.7% at a dose of 800 mg/kg. Furthermore, compounds 8 and 9 obtained from the n -butanol extract exhibited significant anti-inflammatory activities with inhibition rates of 50.9% and 54.7% at a dose of 200 mg/kg.