1. Immunohistochemical investigation of SV40 large T antigen in Hodgkin and non-Hodgkin's lymphoma.
- Author
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Brousset P, de Araujo V, and Gascoyne RD
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Canada epidemiology, Child, Child, Preschool, France epidemiology, Hodgkin Disease etiology, Humans, Immunoenzyme Techniques, Lymphoma, Non-Hodgkin etiology, Middle Aged, Antigens, Polyomavirus Transforming metabolism, Hodgkin Disease metabolism, Lymphoma, Non-Hodgkin metabolism, Simian virus 40 immunology
- Abstract
DNA sequences from simian virus 40 (SV40) have been detected in various human tumors, including non-Hodgkin's lymphomas (NHLs), by highly sensitive PCR techniques. However, there is a strong debate as to whether SV40 is present in lymphoma cells. Using immunohistochemistry and tissue microarrays, we investigated a series of French and Canadian cases of Hodgkin's lymphomas (HLs) and NHLs and tried to detect the SV40 large T antigen using routine paraffin sections with standard and highly sensitive catalyzed system amplification methods. None of the cases of HLs (n = 250) or NHLs (n = 232) were found to contain a single large T antigen-positive cell, whereas 2 positive controls were repeatedly stained. Therefore, our results do not support the hypothesis that SV40 is implicated in the etiology of human lymphomas.
- Published
- 2004
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