1. Heterogeneous nuclear ribonucleoprotein A2/B1 identified as an autoantigen in autoimmune hepatitis by proteome analysis.
- Author
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Huguet S, Labas V, Duclos-Vallee JC, Bruneel A, Vinh J, Samuel D, Johanet C, and Ballot E
- Subjects
- Adolescent, Adult, Aged, Antibodies, Antinuclear analysis, Case-Control Studies, Electrophoresis, Gel, Two-Dimensional, Electrophoresis, Polyacrylamide Gel, Female, Fluorescent Antibody Technique, Indirect, France, Heterogeneous-Nuclear Ribonucleoprotein Group A-B, Humans, Immunoblotting, Male, Mass Spectrometry, Middle Aged, Molecular Weight, Silver Staining, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Autoantigens immunology, Hepatitis, Autoimmune immunology, Proteome analysis, Proteomics methods
- Abstract
Our aim was to characterize antinuclear antibody (ANA) targets in type 1 autoimmune hepatitis (AIH1) using a proteomic tool. ANA-positive sera from 29 patients with AIH1 and 14 negative controls were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. The principal antigens identified were a 30 kDa triplet band which was recognized by 79% of patient sera and 14% of control sera, and a 36 kDa antigen which was recognized by 52% and 14% of sera, respectively. The latter antigen was studied in more detail using two-dimensional electrophoresis (2-DE) and mass spectrometry (MS). Its identification corresponded to heterogeneous nuclear ribonucleoprotein A2/B1. This paper presents the first description of this protein as an antigen in AIH1.
- Published
- 2004
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