1. Clonal emergence of extended-spectrum beta-lactamase (CTX-M-2)-producing Salmonella enterica serovar Virchow isolates with reduced susceptibilities to ciprofloxacin among poultry and humans in Belgium and France (2000 to 2003).
- Author
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Bertrand S, Weill FX, Cloeckaert A, Vrints M, Mairiaux E, Praud K, Dierick K, Wildemauve C, Godard C, Butaye P, Imberechts H, Grimont PA, and Collard JM
- Subjects
- Animals, Anti-Bacterial Agents pharmacology, Bacterial Proteins analysis, Bacterial Proteins genetics, Bacterial Typing Techniques, Belgium, Chromosomes, Bacterial genetics, Cluster Analysis, DNA Fingerprinting, DNA, Bacterial genetics, Electrophoresis, Gel, Pulsed-Field, France, Genome, Bacterial genetics, Genotype, Humans, Meat Products microbiology, Microbial Sensitivity Tests, Plasmids, Polymorphism, Restriction Fragment Length, Poultry, Salmonella enterica classification, Salmonella enterica enzymology, Salmonella enterica isolation & purification, beta-Lactamases genetics, Ciprofloxacin pharmacology, Drug Resistance, Multiple, Bacterial genetics, Poultry Diseases microbiology, Salmonella Infections microbiology, Salmonella Infections, Animal microbiology, Salmonella enterica drug effects, beta-Lactamases biosynthesis
- Abstract
Antibiotic treatment is not required in cases of Salmonella enterica gastroenteritis but is essential in cases of enteric fever or invasive salmonellosis or in immunocompromised patients. Although fluoroquinolones and extended-spectrum cephalosporins are the drugs of choice to treat invasive Salmonella, resistance to these antibiotics is increasing worldwide. During the period 2000 to 2003, 90 Salmonella enterica serovar Virchow poultry and poultry product isolates and 11 serovar Virchow human isolates were found to produce an extended-spectrum beta-lactamase, CTX-M-2, concomitantly with a TEM-1 beta-lactamase. The bla(CTX-M-2) gene was located on a large conjugative plasmid (>100 kb). Pulsed-field gel electrophoresis indicated a clonal relationship between the poultry and human isolates. All these isolates displayed additional resistance to trimethoprim-sulfamethoxazole and tetracycline as well as a reduced susceptibility to ciprofloxacin (MICs of between 0.5 and 1 mug/ml). CTX-M-2-producing Salmonella with a reduced susceptibility to fluoroquinolones constitutes a major concern, since such strains could disseminate on a large scale and jeopardize classical antibiotic therapy in immunocompromised patients.
- Published
- 2006
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