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The main objective of this study was to evaluate the fruit quality characteristics and biochemical properties of Mondial Gala, Jeromine, and Granny Smith cultivars grafted on M9 full-dwarf clonal rootstock and Granny Smith, Fuji, Galaxy Gala, Red Chief, and Scarlett Spur cultivars grafted on MM 106 semi-dwarf clonal rootstock grown in Ordu (Türkiye) ecological conditions. During the research, fruit weight, fruit dimensional characteristics, fruit firmness, color characteristics, soluble solids content, titratable acidity, pH, vitamin C, total phenolic, total flavonoid and antioxidant activity of the cultivars were investigated. In the study, it was determined that, fruit weight, fruit firmness, SSC and Vitamin C 185.40 g (Mondial Gala/M9)- 284.03 g (Scarlet Spur/MM 106), 69.73 N (Galaxy Gala/MM 106)-84.47 N (Granny Smith/M9), 8.29% (Scarlett Spur/MM 106)-14.27% (Fuji/MM 106), 58.00 mg (Scarlet Spur/MM 106)-112.33 mg (Granny Smith/M9), respectively. In addition, it was determined that Granny Smith, Red Chief, Mondial Gala, and Jeromine cultivars stand out in terms of bioactive compounds that positively affect human health compared to other cultivars. As a result, it has been revealed that apple cultivars grafted on M9 and MM 106 clone rootstocks examined in the study can be grown in high quality in Ordu ecology. [ABSTRACT FROM AUTHOR]