1. Three-dimensional printed bulking agents reduce antibiotic resistance genes in swine manure aerobic composting by regulating oxygen concentration to alter host microorganisms and mobile genetic elements.
- Author
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Ma, Shuangshuang and Liu, Hongtao
- Subjects
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MOBILE genetic elements , *SWINE manure , *COMPOSTING , *DRUG resistance in bacteria , *POLLUTANTS , *REDUCING agents , *ANAEROBIC microorganisms - Abstract
[Display omitted] • 3DBAs with regular shape and standardized porosity facilitated oxygen consumption. • Addition of 3DBAs reduced total ARGs, TRGs, MRGs, and SRGs rather than FRGs. • 3DBAs reduced the risk of ARGs by regulating oxygen to alter host microbes and MGEs. Antibiotic resistance genes (ARGs) in manure aerobic composting are a potential environmental pollutant. Therefore, reducing the abundance of ARGs is crucial. The effects of adding three-dimensional printed bulking agents (3DBAs) on ARGs in aerobic composting of swine manure were investigated in this study. Compared with the control group, 3DBAs with different addition dosages can greatest reduce the total ARGs by 5.98%, tetracycline resistance genes by 14.02%, macrolide resistance genes by 9.65%, and sulfonamide resistance genes by 20.59%. By further combining physicochemical parameters, host microorganisms, and mobile genetic elements (MGEs) for analysis, it was found that oxygen concentration was vital for ARGs reduction, and 3DBAs with regular porosity and uniform size indirectly regulate the activity of host microorganisms and MGEs abundance by changing the oxygen consumption, finally reducing vertical or horizontal ARGs transfer risks. Overall, 3DBAs addition is an effective strategy to reduce the abundance of ARGs in aerobic composting. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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