1. Mycobacterium monacense sp. nov.
- Author
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Reischl U, Melzl H, Kroppenstedt RM, Miethke T, Naumann L, Mariottini A, Mazzarelli G, and Tortoli E
- Subjects
- Adult, Aged, 80 and over, Anti-Bacterial Agents pharmacology, Bacterial Proteins genetics, Bronchoalveolar Lavage Fluid microbiology, Chaperonin 60, Chaperonins genetics, Child, DNA, Bacterial chemistry, DNA, Bacterial genetics, DNA, Ribosomal chemistry, DNA, Ribosomal genetics, DNA, Ribosomal Spacer chemistry, DNA, Ribosomal Spacer genetics, Female, Genes, rRNA, Germany, Humans, Italy, Male, Microbial Sensitivity Tests, Molecular Sequence Data, Nontuberculous Mycobacteria physiology, Phylogeny, Pigments, Biological, RNA, Bacterial genetics, RNA, Ribosomal, 16S genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Sputum microbiology, Wound Infection microbiology, Mycobacterium Infections, Nontuberculous microbiology, Mycolic Acids analysis, Nontuberculous Mycobacteria classification, Nontuberculous Mycobacteria isolation & purification
- Abstract
Four bacterial strains were isolated from independent clinical specimens in different countries and their genotypic and phenotypic characters support their classification in a novel species within the genus Mycobacterium. One strain was clearly responsible for a severe, post-traumatic wound infection in a healthy boy. The novel species, for which the name Mycobacterium monacense sp. nov. is proposed, is yellow-pigmented, non-photochromogenic and grows in less than a week on solid medium. Based on phenotypic investigations alone, distinction of these four strains from known scotochromogenic rapidly growing strains is problematic. However, the novel strains differ from any other mycobacterium in each of the molecular species markers investigated: the 16S rRNA gene, the 16S-23S rRNA gene internal transcribed spacer and the hsp65 gene. Of the strains investigated, two different sequevars were detected for the hsp65 region. Phylogenetic analysis revealed that these four strains were most closely related to Mycobacterium doricum. The type strain of Mycobacterium monacense sp. nov. is B9-21-178T (=DSM 44395T=CIP 109237T).
- Published
- 2006
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