1. Characterization of Canine parvovirus 2 variants circulating in Greece.
- Author
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Ntafis V, Xylouri E, Kalli I, Desario C, Mari V, Decaro N, and Buonavoglia C
- Subjects
- Amino Acid Sequence, Animals, Base Sequence, Capsid Proteins genetics, Diarrhea epidemiology, Diarrhea virology, Dog Diseases epidemiology, Dogs growth & development, Dogs virology, Germany, Greece epidemiology, Italy, Parvoviridae Infections epidemiology, Parvovirus, Canine isolation & purification, Polymerase Chain Reaction methods, Polymerase Chain Reaction veterinary, Spain, United States, Diarrhea veterinary, Dog Diseases virology, Genetic Variation, Parvoviridae Infections veterinary, Parvovirus, Canine genetics
- Abstract
The aim of the present study was to characterize Canine parvovirus 2 (CPV-2) variants currently circulating in Greece. Between March 2008 and March 2009, 167 fecal samples were collected from diarrheic dogs from different regions of Greece. Canine parvovirus 2 was detected by standard polymerase chain reaction, whereas minor groove binder probe assays were used to distinguish genetic variants and discriminate between vaccine and field strains. Of 84 CPV-2-positive samples, 81 CPV-2a, 1 CPV-2b, and 2 CPV-2c were detected. Vaccine strains were not detected in any sample. Sequence analysis of the VP2 gene of the 2 CPV-2c viruses revealed up to 100% amino acid identity with the CPV-2c strains previously detected in Europe. The results indicated that, unlike other European countries, CPV-2a remains the most common variant in Greece, and that the CPV-2c variant found in Europe is also present in Greece.
- Published
- 2010
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