1. Multiplex Mediator Displacement Loop-Mediated Isothermal Amplification for Detection of Treponema pallidum and Haemophilus ducreyi.
- Author
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Becherer, Lisa, Knauf, Sascha, Marks, Michael, Lueert, Simone, Frischmann, Sieghard, Borst, Nadine, von Stetten, Felix, Bieb, Sibauk, Adu-Sarkodie, Yaw, Asiedu, Kingsley, Mitjà, Oriol, and Bakheit, Mohammed
- Subjects
TREPONEMA pallidum ,NUCLEIC acid amplification techniques ,HAEMOPHILUS ,GLOBUS pallidus ,RESEARCH ,MOLECULAR diagnosis ,YAWS ,RESEARCH methodology ,CHANCROID ,EVALUATION research ,MEDICAL cooperation ,COMPARATIVE studies ,RESEARCH funding ,BACTERIA - Abstract
Yaws, a neglected tropical disease caused by the bacterium Treponema pallidum subspecies pertenue, manifests as ulcerative skin lesions. Nucleic acid amplification tests, like loop-mediated isothermal amplification (LAMP), are versatile tools to distinguish yaws from infections that cause similar skin lesions, primarily Haemophilus ducreyi. We developed a novel molecular test to simultaneously detect T. pallidum and H. ducreyi based on mediator displacement LAMP. We validated the T. pallidum and H. ducreyi LAMP (TPHD-LAMP) by testing 293 clinical samples from patients with yaws-like lesions. Compared with quantitative PCR, the TPHD-LAMP demonstrated high sensitivity and specificity for T. pallidum (84.7% sensitivity, 95.7% specificity) and H. ducreyi (91.6% sensitivity, 84.8% specificity). This novel assay provided rapid molecular confirmation of T. pallidum and H. ducreyi DNA and might be suitable for use at the point of care. TPHD-LAMP could support yaws eradication by improving access to molecular diagnostic tests at the district hospital level. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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