1. Improving HIV-2 detection by a combination of serological and nucleic acid amplification test assays.
- Author
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Ciccaglione AR, Miceli M, Pisani G, Bruni R, Iudicone P, Costantino A, Equestre M, Tritarelli E, Marcantonio C, Tataseo P, Marazzi MC, Ceffa S, Paturzo G, Altan AM, San Lio MM, Mancinelli S, Ciccozzi M, Lo Presti A, Rezza G, and Palombi L
- Subjects
- Adolescent, Adult, Female, Guinea-Bissau, HIV Infections virology, HIV-1 classification, HIV-1 genetics, HIV-1 immunology, HIV-1 isolation & purification, HIV-2 classification, HIV-2 genetics, HIV-2 immunology, Humans, Immunoassay methods, Male, Middle Aged, Molecular Sequence Data, Nucleic Acid Amplification Techniques methods, RNA, Viral genetics, Sensitivity and Specificity, Sequence Analysis, DNA, Young Adult, HIV Antibodies blood, HIV Infections diagnosis, HIV-2 isolation & purification, RNA, Viral isolation & purification
- Abstract
The ability to detect HIV-2 and to discriminate between HIV-1 and HIV-2 infections was evaluated in 46 serum samples from Guinea-Bissau (GB) and Guinea-Conakry (GC) using serological tests and commercial (HIV-1) and in-house (HIV-2) real-time PCR assays. Samples were first identified as HIV-2 positive by Genie I/II assay in GB and GC. HIV positivity was detected in 44 of 46 samples by all screening and confirmatory assays. A diagnostic strategy based on Inno-LIA and HIV-1/2 RNA detection assays allowed accurate discrimination between HIV-1 and HIV-2 in 84% of single infections and confirmed 32% of double infections. In samples with double reactivity in the Inno-LIA test and no detection of both genomes, cross-reactivity likely hampered the identification of true double infections. In conclusion, the implementation of a diagnostic strategy, based on multiple specific serological tests and highly sensitive quantitative PCR assays, is recommended to ensure accurate HIV-2 diagnosis and appropriate therapy for individuals from areas in which the virus is endemic.
- Published
- 2010
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