1. Characteristics and complete genome sequence of the virulent Vibrio alginolyticus phage VAP7, isolated in Hainan, China.
- Author
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Gao, Mingming, Qin, Yanhong, Fan, Hang, Zhang, Xianglilan, Li, Puyuan, Liu, Huiying, Pei, Guangqian, Mi, Zhiqiang, Xu, Xuling, Tong, Yigang, and Bai, Changqing
- Subjects
VIBRIO alginolyticus ,NUCLEOTIDE sequencing ,BACTERIOPHAGES ,GENETIC transformation ,SEQUENCE analysis ,VIBRIO - Abstract
A novel Vibrio alginolyticus phage, VAP7, was isolated from seawater collected from Sanya, Hainan province, China. Whole-genome sequencing analysis revealed that phage VAP7 has a linear, double-stranded DNA genome of 144,685 bp with an average G+C content of 41.9% and a high degree of sequence similarity to Vibrio phage VP-1. Annotation results identified 193 open reading frames and one transfer RNA-encoding gene in the phage genome. The morphology and the results of phylogenetic analysis suggest that VAP7 should be classified as a new member of the family Ackermannviridae. Moreover, phage VAP7 grew over a wide pH (5.0-10.0) and temperature (4-40 °C) range. Host-range experiments revealed that VAP7 could infect 31 Vibrio alginolyticus strains. Thus, VAP7 infecting Vibrio alginolyticus strains represents a potential new candidate for use in phage therapy. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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