1. Characterization and horizontal transfer of class 1 integrons in Salmonella strains isolated from food products of animal origin
- Author
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Meng, Hecheng, Zhang, Zhigang, Chen, Miaorui, Su, Yongyu, Li, Lin, Miyoshi, Shin-ichi, Yan, He, and Shi, Lei
- Subjects
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SALMONELLA , *MEAT industry , *SEAFOOD contamination , *POLYMERASE chain reaction , *SEROTYPES , *GENETIC transformation - Abstract
Abstract: A total of 81 Salmonella isolates from retail meats and seafood in Hebei province, China, were assayed for the presence of and horizontal transfer of class 1 integrons. By the PCR screening for the integrons, class 1 integron was detected from strains in serotypes of Derby, Indiana, London and Choleraesuis, which were isolated from pork, chicken or seafood; however, two isolates contained the empty integron that lacked the resistance cassette, a potential hotspot for development of the multidrug resistance. In contrast, two other isolates had the antibiotic resistance gene cassettes within the class 1 integron, which were dfrA1-aadA1 and aadB-cmlA, respectively. The conjugation experiments demonstrated the plasmid-mediated transfer of the class 1 integrons. Furthermore, each of the integrons was transmitted to Streptococcus mutans via natural gene transformation. These findings suggest the possible transfer of class 1 integrons from foodborne pathogens to human residential bacteria via horizontal gene transfer. [Copyright &y& Elsevier]
- Published
- 2011
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