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Fixed timed artificial insemination (FTAI) is nowadays a very popular reproductive technology to handle anestrous problems during the postpartum period of dairy cattle. Therefore, the present study was designed to assess the suitable fixed timed AI protocols for therapeutic management of post-partum anestrous in cows. A total of ninety cows were selected in and around the Aizawl district of Mizoram, India which lies within the tropics between latitude 21˚ 56'N to 24˚31'N and longitude 92˚ 16'E to 93˚26'E. The experimental cows were examined per rectum to study the ovarian and genital status after two months of calving. They were randomly divided into three groups; A, B and C, comprising 30 animals in each group and were treated by three fixed timed AI protocols; G6G (PGF day-0; GnRH 2, 8 days; PGF 15, 16 days, GnRH 17 days; FTAI 18 days), select-synch and co-synch, respectively. Blood samples were collected to study the serum levels of glucose, total cholesterol, calcium, phosphorus, estrogen and progesterone on day 0 (before starting the protocol) and day 1 (day before AI). The serum level of glucose, total cholesterol, calcium, phosphorus, estrogen and progesterone significantly low in post-partum anestrous cows on day 0. But after post therapy, the level of glucose, total cholesterol, calcium, phosphorus, estrogen increased significantly except progesterone hormone on day before AI within each group. The conception rates of G6G, co-synch and select-synch protocols were 33.33%, 56.66% and 96.66%, respectively. In conclusion, the select-synch protocol was found to be the best in comparison to G6G and co-synch protocols (P<0.05) for therapeutic management of post-partum anoestrous in cows. [ABSTRACT FROM AUTHOR]