1. Antioxidant Capacities and Total Polyphenol Contents of Hydro-ethanolic Extract of Phytococktail from Trans-Himalaya.
- Author
-
Dhar, P., Tayade, A.B., Bajpai, P.K., Sharma, V.K., Das, S.K., Chaurasia, O.P., Srivastava, R.B., and Singh, S.B.
- Subjects
ANTIOXIDANTS ,PLANT polyphenols ,MEDICINAL plants ,SEA buckthorn ,APRICOT ,OXIDATIVE stress ,TREATMENT of mountain sickness ,THERAPEUTICS - Abstract
The aim of this study was to evaluate the in vitro antioxidant potential of hydro-ethanolic extract of a novel phytococktail comprising of sea buckthorn, apricot, and Rhodiola (SAR) from trans-Himalaya. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) activity of the extract increased in a dose-dependent manner (upto 0.1 mg/mL), and was found to be about 38% of that of ascorbic acid at 0.1 mg/mL. The hydro-ethanolic extract of SAR also scavenged the ABTS
.+ radical generated by ABTS/potassium persulfate (PPS) system and was found to be about 62% of that of ascorbic acid at 0.1 mg/ mL. The total antioxidant power of the extract was determined by ferric reducing antioxidant power (FRAP) assay. Total phenolic content was found to be 1.28016 × 10−3 mol gallic acid equivalent (GAE)/g extract. Total flavonoid and flavonol contents were estimated to be 2.5970 × 10−4 mol and 4.87 × 10−4 mol quercetin equivalent/g extract, respectively. The hydro-ethanolic extract of this phytococktail indicated presence of essential phytoconstituents of polyphenols, flavonoids, flavonols, and ascorbic acid, which contributed significantly to its antioxidant capacity. The combination of the 3 plants may well support their use in traditional medicine to combat oxidative stress and high-altitude sickness. [ABSTRACT FROM AUTHOR]- Published
- 2012
- Full Text
- View/download PDF