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The present study was conducted on 18 dogs of either, sex, breed, aged between 4-14 months brought to Veterinary Clinical Complex and Department of Veterinary Surgery and Radiology, College of Veterinary Science, Rajendranagar, Hyderabad having a femoral diaphyseal fracture in dogs. The dogs were randomly divided into three equal groups irrespective of breed and sex. The radiographs in two standard views were taken to diagnose the fracture and to select the size of titanium implants of Intramedullary Interlocking Nail, Dynamic Compression Plate and Locking Compression Plate for treatment. In all the three groups, the diaphyseal fractures were repaired with internal fixation using Titanium implants. In group I, the femoral fracture was repaired by using the titanium Intramedullary Interlocking Nail. In group II, femoral fracture was repaired by using the titanium Dynamic Compression Plate, while in group III, femoral fractures was repaired by using titanium Locking Compression Plate. A 5 ml blood was collected from each animal on the day of surgery and at different time intervals of 30th, 60th and 90th post-operative day for haemato-biochemical estimation. Haemato-biochemical parameters like haemoglobin, packed cell volume, total erythrocyte count, total leucocyte counts and differential leukocyte count included neutrophil, lymphocyte, eosinophil, and monocyte activity and serum calcium, phosphorus and alkaline phosphatase enzyme activity during fracture healing were analyzed and found that progressive increase in haemoglobin, packed cell volume, total erythrocyte count and progressive decrease in total leukocyte count, differential leukocyte count on different post-operative days within the physiological limits and progressive increase in serum calcium and phosphorus up to 60th post-operative day and then decrease in value and serum alkaline phosphatase were found highest on pre-operative day with gradual decrease up to 90th Post-operative day within the normal physiological limits provided help in assessment of fracture healing in all the three groups of dogs. [ABSTRACT FROM AUTHOR]