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Clayey soils in Raithal, Jalore, Rajasthan, India, have been used for local manufacturing of clay ceramic water filters by potter communities. Potters are trying clayey soils from different locations across India to produce these filters. The suitability of clayey soils from Jheepasani, Jodhpur, and Gajsinghpura, Jodhpur, Rajasthan, India, to manufacture these water filters is compared with soils from Raithal, Jalore, Rajasthan, India. The shrinkage limit and maximum dry density were the lowest for Jheepasani soils. Jheepasani soils have quartz in large quantities, while Gajsinghpura soils are characterized by zeolite. Scanning electron microscopy revealed dense, flaky structure of Gajsinghpura soils and a loosely packed matrix in Jheepasani soils. The micrograph of soils depicts the presence of crystalline character in constituents of Raithal and Gajsinghpura soils. A 3L frustum-shaped composite is made by mixing an equal amount of each of the three soils listed above (taken on their own) with an equal amount of burnout material. The mixture is then pressed into the shape. These press-formed frustums were traditionally baked at 750°C in a potter's open-hearth kiln at Banad village, Jodhpur, Rajasthan, India, to get the new 3L ceramic water filters. A maximum percolation rate of 1.8 L/h was observed from filters manufactured using Jheepasani soils. The samples extracted from the walls of Gajsinghpura soil-based ceramic water filters were comparatively more substantial than those manufactured from the soils of Raithal and Jheepasani. The effectiveness of these 3L filters was tested using Escherichia coli as a surrogate for other waterborne pathogens. E. coli removal with a log reduction value of 2.62 was showcased by Raithal soil-based ceramic water filters. On the basis of this result, Raithal soil-based ceramic water filters were found to be functionally better among the three ceramic water filter variants manufactured from clayey soils found near the Thar Desert in India. [ABSTRACT FROM AUTHOR]

The global agenda of sustainable development (SD) will get a major boost from the successful implementation of the Sustainable Development Goals (SDGs) in developing countries. This requires effective planning and understanding of the SDGs at the national level and developing sound SDG indicators that account for country priorities. Currently, there is no formal SDG indicator set in India, but the process for it is underway. This paper has attempted to propose a unique technique for identifying the most representative indicator set for SD measurement in India based on the SDGs. Considering the needs and preferences of the country, a three‐stage process is proposed to develop the priority indicator set. The application of the approach has been presented for SDG 7, which relates to energy. This is supported with a background on India's SD progress. The proposed technique is simple yet effective, and can be easily replicated by other developing countries. [ABSTRACT FROM AUTHOR]

The Saptashrungi gad temple (SGT) situated on basaltic hills belongs to Deccan volcanic of Upper Cretaceous to Lower Eocene, is one among the 51 Shakti Peeths and most holy place for pilgrims. Rockfall is a major problem in the past and causing danger to the lives of the villagers settled at the toe of the SGT hill as well as the pilgrims who perform parikrama along the tracks. On the evening of 16 April 2011, an old woman died due to rockfall at SGT hill when she was performing parikrama, moreover, two persons got injured during the deliverance process of this old woman from the continuous rockfall activity. The problem of rockfall could be linked to rainfall, jointing, weathering, man-made or the compounding of all. In this research, the rockfall hazard analysis at SGT hill is assessed using both 2D and 3D rockfall programs along the two parikrama paths: Parikrama Path 1 (or the Badi Parikrama Path 'BPP'), and Parikrama Path 2 (or the Chhoti Parikrama Path 'CPP'). Also, the study area of the SGT hill has been divided into eight zones (Zone#01 to Zone#08), based on field observations, orientations of joint sets and hill slope faces and eighteen topographic profiles (AA' to RR') have been taken from these eight zones for rockfall analysis. A detailed topographic survey along with field investigation has been carried out along the temple for ascertaining the nature of rock, discontinuity orientations, and slope geometry. DEM has been generated using topographic profile in ArcGIS to facilitate the 3D rockfall analysis. Maximum rock block sizes are taken into the analysis and run-out distance, bounce height, kinetic energy and velocity of the basaltic blocks are evaluated separately. Based on the analyzed data, the rockfall hazard zone map has been prepared and site having potential rockfall risks have been identified. Finally, wire/net meshing has been proposed after removal of unstable blocks as a stabilization and protection measures. It is worth mentioning here that for the first time rockfall hazard assessment was made in such detail for a site. Suggestions made are implemented by the State Government for the protection of the temple as well as the life of pilgrims performing the parikrama from the rockfall. [ABSTRACT FROM AUTHOR]

This paper mainly describes the assessment of rockfall risk for the large number of pilgrims along the parikrama path (circumbultory or pathway around the shrine) at the Saptashrungi Gad Temple, Vani, Nashik, Maharashtra, India. The temple is constructed on moderately weathered and fractured basaltic terrain along the steep high hill slope. The loose rockmass from the top of the hill occasionally fall, bounce, roll and slide down along the slope of the temple with some rockmass blocks landing on or passing the parikrama path. This leads to risk while taking parikrama along the hill. A quantitative risk assessment (QRA) was undertaken to determine the risk for the pilgrims performing parikrama along the parikrama paths struck by rockfalls and the risk reduction capabilities of various remedial work. The obtained QRA results indicate that, the societal risk cannot be acceptable. [ABSTRACT FROM AUTHOR]

Abstract: Rockfalls are frequent phenomena in hilly regions and have threatened manmade environment as well as generated loss of human life and property. Therefore, the detachment of large size boulders and their fall track represent issues that should be evaluated for urban planning and for the construction of lifelines. In order to achieve this evaluation, several methodologies has been proposed and applied, regarding rockfall hazard. In this paper, a delineation of areas susceptible to rock-falling at the foothills of around the Saptashrungi Gad temple has been investigated. Various cases were simulated and analyzed using numerical tools and pertinent parameters incorporated and calibrated in field and laboratory using silent witnesses. The area mainly comprises compact and amygdaloidal basalt with weak planes of red bole. The outcome of this study is the estimation of simulated fall track and the rockfall bounce height with distance, variation of bounce height with mass of the falling block and dependence of translational kinetic energy on block mass. Bounce height decreases as the mass of the falling block increases whereas translational kinetic energy increases with the mass of the falling block. Also rockfall simulations have been used to know the capacity of the protection barrier and the barrier capacity has found to be 180kJ for 200kg of falling blocks. [Copyright &y& Elsevier]

This article examines a neglected aspect of international librarianship, namely the role of NGOs (nongovernmental organizations) in the development and management of public and community libraries. We present a case study of NGOs operating in neglected and underserved slum areas of the southern Indian metropolis of Bangalore. This case study is based on recent field research the authors have conducted, and provides insights into this alternative model of library development. The qualitative research methods employed in the field research include face-to-face interviews, focus groups, and observations. One of the important findings of this research is that communitybased libraries are viable and important components of a social development agenda in India. However, more research is needed to study the role of NGOs in library development, both in India and in other parts of the developing world. NGOs can be effective service delivery mechanisms, but also can suffer from issues of sustainability, governance, and accountability. Thus while NGOs are reaching some of the most marginalized populations in India through their library services, the government still has an important role to play in library development. An enhanced government-run public library system, in combination with these other NGO services, might present an ideal scenario for serving the largest cross section of the public most effectively. This research confirms the need for and importance of community library services in India. As we move forward, developing larger-scale models and finding best practices in this area should be a concern for future researchers, concerned citizens, library officials, and policymakers. [ABSTRACT FROM AUTHOR]

The growth of slums in urban regions of the Global South is an issue of major concern for a variety of fields. These rapid urban changes are presenting a myriad of challenges, including the provision of social and educational services to urban populations that are often invisible to the gaze of the state. One issue that is not often discussed in slums is the role that educational spaces and places can play in facilitating community development. Specifically, this article discusses the case of community libraries in the slums of Bangalore, India. Community libraries, while only part of a larger empowerment and development strategy, can play an important role in providing a sense of place and belonging. The libraries that are part of this case study are focused on providing services to children, which in certain respects creates wider community support. [ABSTRACT FROM AUTHOR]

Efficacy of sulfoxaflor was evaluated in six field experiments against the cotton jassid, Amrasca devastans (Distant) during 2008 and 2009 in Hyderabad and Multan areas of Pakistan. The data revealed that sulfoxaflor 240SC significantly reduced A. devastans populations upto one week in three trials conducted during 2008. Sulfoxaflor @ 36 g a.i. per hectare provided very good control even at 10 days. Movento 150 OD (spirotetramat) and Confidor 200SL (imidacloprid) at 50 g a.i. per hectare were also highly active on Indian jassid in Hyderabad, but in Multan where the initial pest pressure was high, only neonicotinoid provided some initial activity. In 2009, where the population of A. devastans was very high, initial efficacy after 3 days application and residuality of two neonicotinoids was low, whereas sulfoxaflor 500WDG demonstrated very robust short-term and residual activity when applied @ 25-50 g a.i. per hectare. In all field experiments conducted in Multan and Hyderabad sulfoxaflor at 24-50 g a.i. per hectare clearly exhibited consistent performance against A. devastans and proved as an excellent new tool for managing the most destructive pest of cotton in Pakistan. [ABSTRACT FROM AUTHOR]

This Daylighting is one of the basic components of passive solar building design and its estimation is essential. In India there are a few available data of measured illuminance as in many regions of the world. The Indian climate is generally clear with overcast conditions prevailing through the months of July to September, which provides good potential to daylighting in buildings. Therefore, an analytical model that would encompass the weather conditions of New Delhi was selected. Hourly exterior horizontal and slope daylight availability has been estimated for New Delhi using daylight modeling techniques based on solar radiation data. A model to estimate interior illuminance was investigated and validated using experimental hourly inside illuminance data of an existing skylight integrated vault roof mud house in composite climate of New Delhi. The interior illuminance model was found in good agreement with experimental value of interior illuminance. [ABSTRACT FROM AUTHOR]

In this paper, an attempt has been made to develop a new model to evaluate the hourly solar radiation for composite climate of New Delhi. The comparison of new model for hourly solar radiation has been carried out by using various model proposed by others. The root mean square error (RMSE) and mean bias error (MBE) have been used to compare the accuracy of new and others model. The results show that the ASHRAE and new proposed model estimate hourly solar radiation better for composite climate of New Delhi in comparison to other models. Hourly solar radiation estimated by constants obtained by new model (modified ASHRAE model) for composite climate of India is fairly comparable with measured data. The percentage mean bias error with new constants for New Delhi was found as low as 0.15 and 0% for hourly beam and diffuse radiation, respectively. There is a 1.9–8.5% RMSE between observed and predicted values of beam radiation using new constants for clear days. The statistical analysis has been used for the present study. Copyright © 2008 John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR]

An etiological and epidemiological study of oral submucous fibrosis (OSMF) has been done in Patna, Bihar. Total 157 cases of OSMF and 135 control subjects were selected for study in the period of 2002-2004. It was observed that Male:Female ratio was 2.7:1. The youngest case of OSMF was 11 year old and the oldest one was 54 years of age. Maximum number of cases were belonging to 21-40 years of age and they were belonging to low or middle socioeconomic class. Most of the OSMF cases used heavy spices and chillies, where as control mild spices and chillies. Gutkha was the most commonly used by the OSMF cases only 3 per cent did not use any gutkha or other areca nut product where as 80 per cent control did not have any chewing habit. The OSMF cases used gutkha and other products 2-10 pouches per day and kept in the mouth for 2-10 minutes and they were using since 2-4 years. Most of the OSMF cases kept gutkha in the buccal vestibule or they chewed and swallowed it, only a small number of patients chewed and spitted it out. It was also observed that OSMF developed on one side of the buccal vestibule where they kept the chew and other side was normal. [ABSTRACT FROM AUTHOR]

Evapotranspiration (ET) from irrigated land is one of the most useful indicators to explain whether the water is used as "intended". In this study, the Surface Energy Balance Algorithm for Land (SEBAL) was used to compute actual ET from a Landsat7 image of December 29, 2000 for diverse land use in the Krishna Basin in India. SEBAL ETa varies between 0 to 4.7 mm per day over the image and was quantified for identified land use classes. Seasonal/annual comparison of ETa from different land uses requires time series images, processed by SEBAL. In this study, the Landsat-derived snapshot SEBAL ETa result was interpreted using the cropping calendar and time series analysis of MODIS imagery. The wastewater irrigated area in the basin has the highest ETa in the image, partly due to its advanced growth stage compared to groundwater-irrigated rice. Shrub and forests in the senescence phase have similar ETa to vegetable/cash crops, and ETa from grasslands is a low 0.8 mm per day after the end of the monsoon. The results indicate that wastewater irrigation of fodder and rice is sufficient to meet crop water demand but there appears to be deficit irrigation of rice using groundwater. [ABSTRACT FROM AUTHOR]

A study has been conducted at the Bicholim iron-ore mine, North Goa, India to investigate the hydrogeological continuity between the mine's open pit and surrounding villages. Water resource accounting by demarcating different watersheds has also been carried out to analyse the major causes of groundwater scarcity in the region during dry periods. A resistivity survey and a ground penetrating radar survey were completed around the periphery of open pits, the surrounding village areas and Mayem Lake. The results of the two independent surveys and other related data confirm that there is no observable hydrogeological continuity between the pits, nearby villages and Mayem Lake. Hence, there may not be any significant effect on surrounding water resources due to mining or mine dewatering. Water resource accounting has revealed that only 15–20% of 3500 mm precipitation per annum becomes part of the groundwater resource. The remainder of the annual precipitation is lost as heavy runoff, evapotranspiration and subsurface capillary moisture. Closely spaced wells in the region have also aggravated the groundwater scarcity problem due to draw down effects. Therefore, an effective water resource conservation plan is required for the region to overcome the problem. [ABSTRACT FROM AUTHOR]

This paper deals with the drinking water quality of the Ganga-Kali sub-basin which occupies 1300 km2 over parts of Aligarh and Etah districts. Water samples were collected from shallow and deep aquifers and were analyzed for major ions and trace elements. The analytical data were interpreted according to published guidelines. Chemical analysis shows that the groundwater in the basin is alkali bicarbonate type. Trace element studies of water from the shallow aquifer show that the concentration of toxic metals Fe, Mn, Cd, Pb, and Cr+6 are above permissible limits which may present a health hazard. The water from the deep aquifer is comparatively free from contamination. The aquifers are subject to contamination due to sewage effluents and excessive use of fertilizers and pesticides in agriculture. [ABSTRACT FROM AUTHOR]

Industrial activities are paramount to sustaining the economy in a rapidly developing nation and global powerhouse like India. Leather industries are important in the country's economic map due to the high revenue and employment generation opportunities. Several of these industries contribute largely to environmental pollution. The pollution of the environment is mainly caused by improper disposal of the tannery effluents that are highly rich in hexavalent chromium, a potent human carcinogen. Hexavalent chromium imparts toxic effects on the biotic components, which include plants, animals, and humans. The review portrays the current status of the Indian leather tanning sector and its impact on the Indian economy. The process of chromium tanning and its adverse effects on the environmental biotic components have been briefly discussed. Phytoremediation of these effluents using suitable hyperaccumulating plants has been suggested as an eco-friendly and cost-effective approach for the sustainable restoration of the polluted environment. The mechanism behind the remediation approach and the factors influencing it have been detailed. The manuscript briefly discusses some important advancements in the field of phytoremediation and emerging technologies and concludes by emphasizing further research for sustainable management of tannery wastes., (© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)

In India, Ceramic Water Filters (CWF) named G-filters are becoming a point-of-use household water treatment solution. The filter's reliability may be primarily characterized by the filtrate turbidity and microbial removal efficiency. The temperature and moisture gradients of the location affect its performance. This paper describes a 19-week laboratory investigation of G-filters simulating field conditions. The basic objective during the study was to ascertain the effective useable life of the filter. The discharge rate and turbidity reduction ability of the filters are studied in depth. The G-filters were prepared using locally available salty clay and sawdust taken in equal volume fractions. A total of 30 filters with a 9-liter capacity each were used for the experiments. The results showed that CWFs can reduce turbidity effectively. The influent water used for the study had a mean turbidity of 7.65 NTU, which reduced in the range of 0.03 NTU to 1.03 NTU. Over the course of four months, the average flow rate decreased from 1.94 Lh -1 to 0.93 Lh -1 . Although a soft brushing of filters improved the filtration rate but that too failed in the long run. Brunauer-Emmett-Teller (BET) nitrogen adsorption-desorption measurements were used to determine the variation in pore size of filters with time. An average pore radius of 2.91 nm was found in the original filter that has not been used for filtration experiments. Moreover, an average pore radius of 1.77 nm, 1.21 nm, and 1.22 nm were reported in G-filters through which 427.5 L, 807.5 L, and 1035.5 L, respectively, of water were passed. This reduction in pore size is attributed to the clogging of pores over a period of time. This study aims to collect performance data to establish a scientific model using statistical analysis for implementing CWF technology in developing nations., Competing Interests: Declaration of competing interest There is not conflict of interest among the authors which penning down the paper titled “Assessment of the physiochemical properties and sustainability of locally produced ceramic G-filter in a long term experiment”., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Background: Wild food plants (WFPs) play an important role in the traditional dietary habits of various indigenous communities worldwide, particularly in mountainous regions. To understand the dynamics of food preferences, cross-cultural studies on food plants should be conducted across diverse ethnic groups in a given area. In this context, the current study investigated the use of WFPs by seven different cultural groups in the Kashmir Himalayan Region. In this area, people gather wild plants and their parts for direct consumption, traditional foods, or sale in local markets. Despite this reliance, documentation of the food system, especially concerning WFPs, is notably lacking. Hence, our research aimed to document WFPs, along with associated traditional ecological knowledge, and identify major threats to their long-term sustainability in Division Muzaffarabad., Methods: Through a comprehensive approach involving questionnaires, interviews, focus groups, and market surveys, we gathered data from 321 respondents. PCA was performed to analyze threats and plant use using "factoextra" in R software. Origin Pro was used to create a chord diagram, while R software was used to generate a Polar heat map. Additionally, a Venn diagram was created using Bioinformatics software., Results: The study included 321 informants, of whom 75.38% were men and 24.61% were women. In total, 113 plant taxa from 74 genera and 41 botanical families were reported. Polygonaceae and Rosaceae accounted for the majority (17 species each), followed by Lamiaceae (7 species). Leaves were the most used part as food sources (41.04%), followed by fruits (33.33%). Most of the species are consumed as cooked (46.46%) and as raw snacks (37.80%). A total of 47 plant species were collected and cooked as wild vegetables, followed by 40 species used as fruits. This study is the first to describe the market potential and ecological distribution of WFPs in the study area. Cross-comparison showed that utilization of WFPs varies significantly across the region and communities, including their edible parts and mode of consumption. Jaccard index (JI) value ranged from 5.81 to 25. Furthermore, the current study describes 29 WFPs and 10 traditional food dishes that have rarely been documented in Pakistan's ethnobotanical literature. Climate change, invasive species, expansion of agriculture, and plant diseases are some of the most significant threats to WFPs in the study area., Conclusions: The older age group has more knowledge about WFPs compared to the younger generation, who are not interested in learning about the utilization of WFPs. This lack of interest in information about WFPs among the younger generation can be attributed to their limited access to markets and availability of food plants in the study area. Traditional gathering of food plants has been reduced in younger generations during recent years; therefore, it is crucial to develop effective conservation strategies. These efforts not only safeguard indigenous flora, food knowledge, and cultural heritage, but they also contribute to food security and public health by utilizing local wild foods in the examined area., (© 2024. The Author(s).)

Repeated serological testing tells about the change in the overall infection in a community. This study aimed to evaluate changes in antibody prevalence and kinetics in a closed cohort over six months in different sub-populations in India. The study included 10,000 participants from rural and urban areas in five states and measured SARS-CoV-2 antibodies in serum in three follow-up rounds. The overall seroprevalence increased from 73.9% in round one to 90.7% in round two and 92.9% in round three. Among seropositive rural participants in round one, 98.2% remained positive in round two, and this percentage remained stable in urban and tribal areas in round three. The results showed high antibody prevalence that increased over time and was not different based on area, age group, or sex. Vaccinated individuals had higher antibody prevalence, and nearly all participants had antibody positivity for up to six months., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2023 Misra et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

Outbreaks of HFMD in children aged <5 years have been reported worldwide and the major causative agents are Coxsackievirus (CV) A16, enterovirus (EV)-A71 and recently CVA6. In India, HFMD is a disease that is not commonly reported. The purpose of the study was to identify the enterovirus type(s) associated with large outbreak of Hand, foot, and mouth disease during COVID-19 pandemic in 2022. Four hundred and twenty five clinical samples from 196-suspected cases were collected from different parts of the country. This finding indicated the emergence of CVA6 in HFMD along with CVA16, soon after the gradual easing of non-pharmaceutical interventions during-pandemic COVID-19 and the relevance of continued surveillance of circulating enterovirus types in the post-COVID pandemic era., (Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)

Background: COVID-19 was declared as a Public Health Emergency of International Concern on 30th January 2020. Compared to the general population, healthcare workers and their families have been identified to be at a higher risk of getting infected with COVID-19. Therefore, it is crucial to understand the risk factors responsible for the transmission of SARS-CoV-2 infection among health workers in different hospital settings and to describe the range of clinical presentations of SARS-CoV-2 infection among them., Methodology: A nested case-control study was conducted among healthcare workers who were involved in the care of COVID-19 cases for assessing the risk factors associated with it. To get a holistic perspective, the study was conducted in 19 different hospitals from across 7 states (Kerala, Tamil Nadu, Andhra Pradesh, Karnataka, Maharashtra, Gujarat, and Rajasthan) of India covering the major government and private hospitals that were actively involved in COVID-19 patient care. The study participants who were not vaccinated were enrolled using the incidence density sampling technique from December 2020 to December 2021., Results: A total of 973 health workers consisting of 345 cases and 628 controls were recruited for the study. The mean age of the participants was observed to be 31.17 ± 8.5 years, with 56.3% of them being females. On multivariate analysis, the factors that were found to be significantly associated with SARS-CoV-2 were age of more than 31 years (adjusted odds ratio [aOR] 1.407 [95% CI 1.53-1.880]; p  = 0.021), male gender (aOR 1.342 [95% CI 1.019-1.768]; p  = 0.036), practical mode of IPC training on personal protective equipment (aOR 1. 1.935 [95% CI 1.148-3.260]; p  = 0.013), direct exposure to COVID-19 patient (aOR 1.413 [95% CI 1.006-1.985]; p  = 0.046), presence of diabetes mellitus (aOR 2.895 [95% CI 1.079-7.770]; p  = 0.035) and those received prophylactic treatment for COVID-19 in the last 14 days (aOR 1.866 [95% CI 0.201-2.901]; p  = 0.006)., Conclusion: The study was able to highlight the need for having a separate hospital infection control department that implements IPC programs regularly. The study also emphasizes the need for developing policies that address the occupational hazards faced by health workers., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 George, Tomy, Retnakumar, Narlawar, Bhardwaj, Krishnan, Rao, Patel, Bilimale, Baby, Mathew, Cassini, Simniceanu, Yin, Allegranzi, Ahmad, Rahman, Mohiuddin, Thakre, Bhansali, Vohra, Krishnan, Logaraj, Maheriya, Gharat, Dipu, Solomon, Sharma, Shwethashree, Hegde, Ansari and Misra.)

Introduction: Healthcare workers (HCW) are most vulnerable to contracting COVID-19 infection. Understanding the extent of human-to-human transmission of the COVID-19 infection among HCWs is critical in managing this infection and for policy making. We did this study to estimate new infection by seroconversion among HCWs in recent contact with COVID-19 and predict the risk factors for infection., Methods: A cohort study was conducted at a tertiary care COVID-19 hospital in New Delhi during the first and second waves of the COVID-19 pandemic. All HCWs working in the hospital during the study period who came in recent contact with the patients were our study population. The data was collected by a detailed face-to-face interview, serological assessment for anti- COVID-19 antibodies at baseline and end line, and daily symptoms. Potential risk factors for seroprevalence and seroconversion were analyzed by logistic regression keeping the significance at p<0.05., Results: A total of 192 HCWs were recruited in this study, out of which 119 (62.0%) were seropositive. Almost all were wearing Personal protective equipment (PPE) and following Infection prevention and control (IPC) measures during their recent contact with a COVID-19 patient. Seroconversion was observed among 36.7% of HCWs, while 64.0% had a serial rise in the titer of antibodies during the follow-up period. Seropositivity was negatively associated with being a doctor (odds ratio [OR] 0.35, 95% Confidence Interval [CI] 0.18-0.71), having COVID-19 symptoms (OR 0.21, 95% CI 0.05-0.82), having comorbidities (OR 0.14, 95% CI 0.03-0.67), and received IPC training (OR 0.25, 95% CI 0.07-0.86), while positively associated with partial (OR 3.30, 95% CI 1.26-8.69), as well as complete vaccination for COVID-19 (OR 2.43, 95% CI 1.12-5.27). Seroconversion was positively associated with doctor as a profession (OR 13.04, 95% CI 3.39-50.25) and with partially (OR 4.35, 95% CI 1.07-17.65), as well as fully vaccinated for COVID-19 (OR 6.08, 95% CI 1.73-21.4). No significant association was observed between adherence to any IPC measures and PPE adopted by the HCW during the recent contact with COVID-19 patients and seroconversion., Conclusion: Almost all the HCW practiced IPC measures in these settings. High seropositivity and seroconversion are most likely due to concurrent vaccination against COVID-19 rather than recent exposure to COVID-19 patients. Further studies using anti-N antibodies serology may help us find the reason for the seropositivity and seroconversion among HCWs., Competing Interests: NO authors have competing interests., (Copyright: © 2023 Dudeja et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

Background: India did phased measles-rubella supplementary immunisation activities (MR-SIAs; ie, mass-immunisation campaigns) targeting children aged 9 months to less than 15 years. We estimated measles-rubella seroprevalence before and after the MR-SIAs to quantify the effect on population immunity and identify remaining immunity gaps., Methods: Between March 9, 2018 and March 19, 2020 we did community-based, cross-sectional serosurveys in four districts in India before and after MR-SIAs. 30 villages or wards were selected within each district, and one census enumeration block from each was selected as the survey cluster. Households were enumerated and 13 children in the younger age group (9 months to <5 years) and 13 children in the older ager group (5 to <15 years) were randomly selected by use of computer-generated random numbers. Serum samples were tested for IgG antibodies to measles and rubella viruses by enzyme immunoassay., Findings: Specimens were collected from 2570 children before the MR-SIA and from 2619 children afterwards. The weighted MR-SIA coverage ranged from 73·7% to 90·5% in younger children and from 73·6% to 93·6% in older children. Before the MR-SIA, district-level measles seroprevalence was between 80·7% and 88·5% among younger children in all districts, and between 63·4% and 84·5% among older children. After the MR-SIA, measles seroprevalence among younger children increased to more than 90% (range 91·5 to 96·0) in all districts except Kanpur Nagar, in which it remained unchanged 80·4%. Among older children, measles seroprevalence increased to more than 90·0% (range 93·7% to 96·5%) in all districts except Hoshiarpur (88·7%). A significant increase in rubella seroprevalence was observed in all districts in both age groups, with the largest effect in Dibrugarh, where rubella seroprevalence increased from 10·6% to 96·5% among younger children., Interpretation: Measles-rubella seroprevalence increased substantially after the MR-SIAs but the serosurvey also identified remaining gaps in population immunity., Funding: The Bill & Melinda Gates Foundation and Indian Council of Medical Research., Competing Interests: Declaration of interests KH has been an employee by Pfizer Vaccines from October 26, 2020. All other authors declare no competing interests., (Copyright © 2022 The Author(s). Published by Elsevier Ltd. This is an Open Access article under the CC BY 4.0 license. Published by Elsevier Ltd.. All rights reserved.)

The demand for water is increasing around the world due to population growth, urbanization, industrialization, etc., which is making groundwater a vital natural resource for meeting the growing demand for water. According to the central groundwater report, Jammu district has adequate groundwater potential (GWP) and comes under the safe category. However, the GWP has not been fully utilized, thereby leading to a water shortage in the district. Therefore, this study has been designed to examine the GWP zones in the Tawi River basin of Jammu district using geospatial techniques. For this, several GWP conditioning parameters, such as elevation, slope, geology, geomorphology, rainfall, soil, land use/land cover, topographic wetness index (TWI), drainage density, lineament density, roughness, and curvature, were utilized. The analytical hierarchy process (AHP) technique was used to evaluate the weights of the selected criteria after a pair-wise comparison of each criterion with the rest of the criteria. The result showed that the high GWP zone accounts for 21.98% of the area, the moderate zone covers an area of 40.54%, the low GWP area accounts for about 34.91%, and only 2.57% of the area lies under the very low GWP zone. The validation of GWP zones using 25 monitoring wells showed an accuracy of 80% in GWP modeling. The findings of this study may be utilized in meeting the rising demand for water in the region., (© 2022. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)

The studies related to air pollutants and their association with human health over the mountainous region are of utmost importance and are sparse especially over the Himalayan region of India. The linkages between various atmospheric variables and clinically validated data have been done using various datasets procured from satellite, model reanalysis, and surface observations during 2013-2017. Aerosol optical depth, air temperature, and wind speed are significantly related (p < 0.001) to the incidence of acute respiratory infections with its peak during winter. Model-derived particulate matter (PM 2.5 ) shows high contributions of black carbon, organic carbon, and sulfate during winter. The wind roses show the passage of winds from the south-west and southern side of the region. Back trajectory density plot along with bivariate polar plot analyses have shown that most of the winds coming from the western side are taking a southward direction before reaching the study area and may be bringing pollutants from the Indo-Gangetic Plain and other surrounding regions. Our study shows that the accumulation of pollutants in the Himalayan valley is owing to the meteorological stability with significant local emissions from burning of biomass and biofuels along with long-range and mid-range transport during the winter season that significantly correlated with the incidence of acute respiratory infections in the region., (© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Objective: The neurological manifestations and their severity in patients on antiretroviral treatment (ART) are currently unexplained. ‎We aimed at studying the prevalence of HIV Associated Neurological Disorders (HAND) among people on antiretroviral treatment, using the International HIV Dementia Scale (IHDS)., Patients and Methods: A predesigned and pretested proforma including the International HIV Dementia Scale (IHDS) was administered to 100 HIV patients attending to ART center of KIMS teaching Hospital (Koppal, Karnataka) from January 2020 to March 2020. The data was analyzed SPSS version 15 software. Descriptive statistics were used for demographic characteristics. The Student's t-test and chi-square test methods were applied to determine the relationship between qualitative characteristics., Results: The prevalence was found to be 59%. Out of 100, 57 HIV patients scored less than 10 whereas 43 HIV patients scored ≥10 on the IHDS scale. The mean age of the study population was 39.14 ±13.01 years; the total IHDS score was 9.96±1.53 and the CD4 count was 427.91±226.0. This study demonstrated that the patients with CD4 count more than 350 (i.e., 63.60%) had a better IHDS score., Conclusions: Neurocognitive disorder was found to be more common than anticipated. All ICTC Centers need to consider assessing HIV-associated neurocognitive disability (HAND), and the International HIV Dementia Scale (IHDS) as one instrument for such assessment.

The current coronavirus (COVID-19) pandemic has a high spreading and fatality rate. To control the rapid spreading of the COVID-19 virus, the government of India imposed lockdown policies, which creates a unique opportunity to analyze the impact of lockdown on air quality in the two most populous cities of India, i.e., Delhi and Mumbai. To do this, the study employed a spatial approach to examine the concentration of seven criteria pollutants, i.e., PM 2.5 , PM 10 , NH 3 , CO, NO 2 , O 3 , and SO 2, before, during, and after a lockdown in Delhi and Mumbai. Overall, around 42%, 50%, 21%, 37%, 53%, and 41% declines in PM 2.5 , PM 10 , NH 3 , CO, NO 2 , and SO 2 were observed during the lockdown period as compared to previous years. On the other hand, a 2% increase in O 3 concentration was observed. However, the study analyzed the National Air Quality Index (NAQI) for Delhi and Mumbai and found that lockdown does not improve the air quality in the long term period. Our key findings provide essential information to the cities' administration to develop rules and regulations to enhance air quality., (Copyright © 2021 Elsevier Inc. All rights reserved.)

Since developing countries experience economic and environmental sustainability challenges, it is desirable digging into the linkages between economic and environmental parameters. The purpose of this work is to evaluate the existence of the environmental Kuznets curve (EKC) theory (i.e., the inverse U-shape connection between real GDP per capita and per capita carbon dioxide emissions) in the sample of 11 developing countries. By using balanced annual panel data in the period between 1992 and 2014 and two alternative estimation techniques, we explored the potential inverted U-shaped linkage between carbon dioxide emissions and real GDP per capita in the sample of interest. For analysis purposes, Pedroni and Westerlund co-integration techniques are employed. Then, fully modified ordinary least squares, pooled mean group methods are applied for long-run parameter estimations. And, the Dumitrescu-Hurlin causality approach is employed for causal directions. Firstly, this work's findings provide the supportive evidence to the inverse U-shaped linkage in the long-run, indicating that an increase in real GDP per capita and electricity consumption tends to mitigate long-run carbon dioxide emissions in the developing countries, for the whole sample. Secondly, the country-specific findings suggested the presence of EKC theory for Brazil, China, India, Malaysia, the Russian Federation, Thailand, and Turkey. It implicated that these countries are on the path of attaining environmental sustainability in the long-run. However, Mexico, Philippines, Indonesia, and South Africa failed to lend credence to the EKC theory. It manifested that these countries need to design strategies directed to reduce carbon dioxide emissions from economic activity and electricity generation through efficiency improvement or promotion of renewables. Finally, bidirectional causal links are observed among all the variables of interest. The findings suggest that country-specific targeted action plans should be implemented to ensure the environmental sustainability in the developing world.

Objective: To establish hospital-based surveillance to identify cases of rotavirus (RV) among children < 5 y of age hospitalized for acute gastroenteritis (AGE) and to determine the burden and profile of circulating RV genotypes in the region., Methods: This study was conducted at a tertiary level hospital in Bijnor district of western Uttar Pradesh, India from January 2018 to January 2020. The duly filled case reporting forms and specimens of all the enrolled children were transported in cold chain to the referral laboratory at Christian Medical College (CMC), Vellore on a monthly basis for testing and storage of stool samples as well as data entry and analysis., Results: A total of 1055 under-5 children admitted with AGE, were enrolled. Proper stool specimens were collected from 932 children. Rotavirus was found positive in 368 (39.5%) stool specimens. Marked seasonality was observed in RV-positive cases with the highest incidence was noticed during winter months. The 0-11 mo age group had the highest incidence of RV-GE followed by 12-23 mo. G1 (42.08%) was the most frequent G-type whereas G1P[8] (26.23%) was the commonest circulating genotype., Conclusion: The study confirms a significant burden of RV among AGE cases in young children in western Uttar Pradesh. The findings of the study may serve as useful baseline information to the Government of India for assessing vaccine performance after its introduction in the national immunization programmes.

Introduction: This study assessed the seroprevalence against all three polioviruses among the last cohort of infants aged 6-11 months who received tOPV before the tOPV-bOPV switch and had an opportunity to receive a full dose of inactivated poliovirus vaccine introduced in the routine immunization schedule., Methods: Serum was tested for neutralizing antibodies against polioviruses among infants residing in three different risk- category states for poliovirus transmission in India viz., Bihar historically high-risk state for polio, Madhya Pradesh a State with low routine immunization coverage and Chhattisgarh with lower acute flaccid paralysis surveillance indicators., Results: A total of 1113 serum samples were tested across the three states. The overall seroprevalence was 98.5% (97.7-99.2), 98.9% (98.3-99.5) and 94.4% (93.0-95.8) for poliovirus types 1, 2 and 3 respectively. The median antibody titers for corresponding serotypes were 575, 362 and 181. Infants who received five doses of tOPV showed respective seroprevalence rates of 98.7%, 98.7% and 93.7% against types 1, 2 and 3 polioviruses. There was no significant difference in seroprevalence across the group of IPV recipients. The median reciprocal titers across the groups of IPV recipient was significantly higher (p = 0.006) for poliovirus-3., Conclusion: The seroprevalence rates observed in the study are historically the highest in the series of serosurveys that India has conducted to assess the population immunity against polioviruses. Poliovirus 2 seroprevalence was very high at the time of the tOPV-bOPV switch in India effected in April 2016., (Copyright © 2020. Published by Elsevier Ltd.)

Venturia inaequalis is a notorious fungal pathogen and show classical gene for gene interaction with its apple host. Neutral markers provide clues about history, evolutionary potential, genetic diversity and population structure of V. inaequalis. The genetic diversity and population structure of fungus indicates that the pathogen is highly diverse with the capacity to breach the scab resistance genes. In the present study, we collected 108 V. inaequalis isolates from three apple cultivars differing in Rvi1 resistance gene. Based on the AMOVA, the variation was mostly distributed among the isolates, providing evidence of non-existence of subpopulation in orchards thus founder population is difficult to arise in Kashmir apple orchards. Pair wise genetic differentiation is less due to regular occurrence of gene flow between the populations residing on different orchard as infected material is transported without stringent quarantine measures. Based on principal coordinate analysis and clustering algorithm as implemented in STRUCTURE, we observed admixture between the two subpopulations, which is quite low, suggesting the existence of pre-zygotic and post-zygotic barriers to gene flow and we cannot rule out the existence of other structures shared by accessions belonging to different varieties. Due to the continuous increase in introduction and monoculture of apple varieties, mixed orchard with different host resistance specificities are more suitable for managing the apple scab in Kashmir valley.

Ethnopharmacological Relevance: Unani system of medicine uses drugs from plants, minerals and animals (Mawaleed-e-salasa) origin. Most of the drugs used are believed to be safe, but some drugs may have toxins and produce harmful effects, so it is very important to remove the toxins or to minimize their harmful effects before using, so as to increase their therapeutic values and make easy for use., Aim of the Study: To explore the concept, aims and objectives of islah-e-advia (rectification/purification of drugs) in Unani system of medicine., Materials and Methods: A through literature survey was undertaken using the various online bibliographic databases like Pub Med, Google Scholar, Science Direct, Web of Science and Scopus. Classical Unani literature books which are translated in Urdu by CCRUM were used for Unani references. The keywords used for the search included "Tadbeer-e-Advia", "Islah-e-advia", Mudabbar process, detoxification and purification of drug., Results: The ancient Unani physicians were well aware of toxicity of the drugs and have divided drugs into four degrees (1°, 2°, 3° and 4°) according to the Mizaj (temperament) of drugs. The fourth degree drugs produce so strong effects that the physiological functions of the body get disturbed; such drugs are also considered to be poisonous drugs. Different methods of purification have been mentioned for drugs belonging to the third and fourth degree temperament and always recommended their use after subjecting them to certain Tadbeer (rectification/purification). Otherwise these drugs may destroy the physiological function of the body. After purification process the drugs become physically and chemically pure, therapeutically more effective and less toxic. Very few pharmacological and toxicity studies have been conducted., Conclusion: Third and fourth degree drugs and its formulations have been claimed by Unani scholars to be useful in the management of various diseases of the body. After purification toxicity has been reduced in comparison crude drug. However, further elaborate and comprehensive studies are required to validate such claims., Competing Interests: Declaration of competing interest There is no conflict of interest to declare., (Copyright © 2020 Elsevier B.V. All rights reserved.)

Mountains are definitely the most rugged, yet frail resources and biodiversity-rich regions of the world. Environmental variables directly affect species composition, growth patterns, and the ecosystem resulting in a drastic change in the vegetation composition along ascending elevations. The present study investigated vegetation composition, nestedness, and turnover in plant communities along a vertical gradient (2000 to 4000 m) in Dhauladhar Mountains, Lesser Himalayas, India. We determined how α-diversity pattern and nestedness-related processes or turnover (β-diversity) causes dissimilarity in plant communities' composition along the vertical gradient. Overall, 21 permanent plots (20 × 20 m 2 ) at every 100 m interval from 2000 to 4000 m were established. A sampling of shrubs and herbaceous species was done by marking sub-plots of 5 × 5 m 2 and 1 × 1 m 2 , respectively, within permanent plots. We observed an inverted hump-shaped pattern for evenness index (E), a unimodal hump-shaped pattern for Shannon index (H'), Margalef's richness index, and β-Whittaker (β w ) diversity, and mild-hump-shaped pattern for Simpson index (λ) across the elevational gradient. Turnover (β sim ) and the nestedness-resultant component of β-diversity (β sne ) significantly differed across the elevational gradient. The observed β-diversity patterns revealed that the species replacement rate was less in the mid-altitude communities as compared to lower and higher altitude communities. It was largely attributed to the ecotonic nature of mid-altitudes, which benefited mid-elevational communities rather than low or high altitude communities. Besides lower altitudes, the increased human interference has led to disturbance and subsequent homogenization of flora across the mid-altitudes. With respect to this, the present study signifies the need for preserving the mid-altitudinal communities, without undermining the importance of conserving the low and high altitude communities., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier B.V. All rights reserved.)

In this paper, we investigated the association between CO 2 emissions, remittances, income, energy use, and foreign direct investment (FDI) through panel cointegration analysis, common correlated effect mean group (CCEMG), fully modified least squares (FM-LS) estimates, and panel causality test for the Brazil, Russia, India, China, and South Africa (BRICS) economies over 1986-2016. The empirical outcomes of CCEMG and FM-LS clarified that remittance inflows into BRICS region are contributing to environmental degradation. Similarly, results in the case of Brazil, Russia, and China supported the remittances-led emission hypothesis. On the other hand, different results have been reached in India, thus the remittances; inflows have dictated that they are helping to combat CO 2 emission. Furthermore, FDI has a positive and significant sign for BRICS economies. This relationship narrated that the FDI inflows to this region increase the CO 2 emission, hence obeying the pollution haven hypothesis. Finally, energy use also supports the energy-led emanation phenomenon in BRICS panel and alongside the countries. The panel causality test confirms the bidirectional linkage between remittances and CO 2 emissions.

The study's main purpose is to investigate the complex interaction between innovation, renewable energy consumption, and CO 2 emissions (CO 2 e), under the Kuznets curve framework, for BRICS economies from 1980 to 2016. The empirical estimates drwan from the CCEMG technique highlighted the heterogeneous role of innovation. The results indicated that innovation activities have failed to disrupt CO 2 e in China, India, Russia, and South Africa, except for Brazil. Second, the data showed that renewable energy consumption has mitigated CO 2 e in the BRICS panel, Russia, India, and China, excluding South Africa. Third, the existence of the EKC hypothesis was confirmed in all the BRICS economies, excluding India and South Africa. Fourth, the causality estimations reflected a two-way causality between innovation and CO 2 e; innovation and GDP per capita; innovation and renewable energy consumption; and between CO 2 e and income, thereby confirming the acceptance of income-led emission hypothesis in for BRICS economies, and vice versa.

Vitamin B12 is an important cofactor in one-carbon metabolism whose dysregulation is associated with various clinical conditions. Indians have a high prevalence of B12 deficiency but little is known about the genetic determinants of circulating B12 concentrations in Indians. We performed a genome-wide association study in 1001 healthy participants in the Pune Maternal Nutrition Study (PMNS), replication studies in 3418 individuals from other Indian cohorts and by meta-analysis identified new variants, rs3760775 (P = 1.2 × 10-23) and rs78060698 (P = 8.3 × 10-17) in FUT6 to be associated with circulating B12 concentrations. Although in-silico analysis replicated both variants in Europeans, differences in the effect allele frequency, effect size and the linkage disequilibrium structure of credible set variants with the reported variants suggest population-specific characteristics in this region. We replicated previously reported variants rs602662, rs601338 in FUT2, rs3760776, rs708686 in FUT6, rs34324219 in TCN1 (all P < 5 × 10-8), rs1131603 in TCN2 (P = 3.4 × 10-5), rs12780845 in CUBN (P = 3.0 × 10-3) and rs2270655 in MMAA (P = 2.0 × 10-3). Circulating B12 concentrations in the PMNS and Parthenon study showed a significant decline with increasing age (P < 0.001), however, the genetic contribution to B12 concentrations remained constant. Luciferase reporter and electrophoretic-mobility shift assay for the FUT6 variant rs78060698 using HepG2 cell line demonstrated strong allele-specific promoter and enhancer activity and differential binding of HNF4α, a key regulator of expression of various fucosyltransferases. Hence, the rs78060698 variant, through regulation of fucosylation may control intestinal host-microbial interaction which could influence B12 concentrations. Our results suggest that in addition to established genetic variants, population-specific variants are important in determining plasma B12 concentrations., (© The Author 2017. Published by Oxford University Press.)

Objective: The primary aim of the present study was to evaluate the complications, particularly conduction blocks, subsequent morbidity and mortality, and effect of thrombolytic therapy in Indian patients with inferior wall myocardial infarction (IWMI)., Methods: This was a prospective, observational, single-center study conducted at LPS Institute of Cardiology, Kanpur, from December 2011 to May 2014. Patients who presented with typical chest pain and were subsequently diagnosed by standardized diagnostic criteria as having IWMI were enrolled. Patients were grouped on basis of conduction abnormalities, right ventricular (RV) infarction and thrombolytic treatment. Each group was analyzed for comparison of complication profile and mortality., Results: Of 573 patients with IWMI enrolled in the study (mean age: 58.90±12.3 years), 81.2% were male, 225 (39.3%) had conduction blocks, and 189 (32.9%) had RV infarction. In patients with conduction blocks, mortality occurred in 27 patients (12.0%) in contrast to 3.4% of patients without conduction block (p<0.03). Also, there were 27 cases of in-hospital mortality in patients with RV infarction compared with 9 cases in patients without RV infarction (p<0.01). Thrombolytic therapy significantly reduced mortality in patients with IWMI (p<0.001). A significant reduction was observed in cardiogenic shock (p=0.002), severe mitral regurgitation (p=0.007), and left ventricular failure (p<0.001) in patients undergoing thrombolytic therapy., Conclusion: In Indian patients with IWMI, incidence of conduction blocks was higher than previously reported studies. Major complications such as atrioventricular block and RV infarction are associated with increased mortality and poor clinical outcomes. Thrombolytic therapy has a beneficial role in reduction of mortality rate and other complications.

Withania somnifera, also called 'Indian ginseng', is an important medicinal plant of the Indian subcontinent. It is widely used, singly or in combination, with other herbs against many ailments in Indian Systems of Medicine since time immemorial. Withania somnifera contains a spectrum of diverse phytochemicals enabling it to have a broad range of biological implications. In preclinical studies, it has shown anti-microbial, anti-inflammatory, anti-tumor, anti-stress, neuroprotective, cardioprotective, and anti-diabetic properties. Additionally, it has demonstrated the ability to reduce reactive oxygen species, modulate mitochondrial function, regulate apoptosis, and reduce inflammation and enhance endothelial function. In view of these pharmacologic properties, W. somnifera is a potential drug candidate to treat various clinical conditions, particularly related to the nervous system. In this review, we summarize the pharmacologic characteristics and discuss the mechanisms of action and potential therapeutic applications of the plant and its active constituents.

Inactivated poliovirus vaccine (IPV) is efficacious against paralytic disease, but its effect on mucosal immunity is debated. We assessed the efficacy of IPV in boosting mucosal immunity. Participants received IPV, bivalent 1 and 3 oral poliovirus vaccine (bOPV), or no vaccine. A bOPV challenge was administered 4 weeks later, and excretion was assessed 3, 7, and 14 days later. Nine hundred and fifty-four participants completed the study. Any fecal shedding of poliovirus type 1 was 8.8, 9.1, and 13.5% in the IPV group and 14.4, 24.1, and 52.4% in the control group by 6- to 11-month, 5-year, and 10-year groups, respectively (IPV versus control: Fisher's exact test P < 0.001). IPV reduced excretion for poliovirus types 1 and 3 between 38.9 and 74.2% and 52.8 and 75.7%, respectively. Thus, IPV in OPV-vaccinated individuals boosts intestinal mucosal immunity., (Copyright © 2014, American Association for the Advancement of Science.)

Background: Updated estimates of measles case fatality rates (CFR) are critical for monitoring progress towards measles elimination goals. India accounted for 36% of total measles deaths occurred globally in 2011. We conducted a retrospective cohort study to estimate measles CFR and identify the risk factors for measles death in Bihar-one of the north Indian states historically known for its low vaccination coverage., Methods: We systematically selected 16 of the 31 laboratory-confirmed measles outbreaks occurring in Bihar during 1 October 2011 to 30 April 2012. All households of the villages/urban localities affected by these outbreaks were visited to identify measles cases and deaths. We calculated CFR and used multivariate analysis to identify risk factors for measles death., Results: The survey found 3670 measles cases and 28 deaths (CFR: 0.78, 95% confidence interval: 0.47-1.30). CFR was higher among under-five children (1.22%) and children belonging to scheduled castes/tribes (SC/ST, 1.72%). On multivariate analysis, independent risk factors associated with measles death were age <5 years, SC/ST status and non-administration of vitamin A during illness. Outbreaks with longer interval between the occurrence of first case and notification of the outbreak also had a higher rate of deaths., Conclusions: Measles CFR in Bihar was low. To further reduce case fatality, health authorities need to ensure that SC/ST are targeted by the immunization programme and that outbreak investigations target for vitamin A treatment of cases in high risk groups such as SC/ST and young children and ensure regular visits by health-workers in affected villages to administer vitamin A to new cases.

Hypertensive patients have higher prevalence of insulin resistance and are at increased risk of developing type 2 diabetes mellitus (DM). There is scarcity of data on the relationship between antihypertensive therapies and glycaemic control in Indian population. Thus, the present study was designed to investigate such association among Indian population in a University teaching hospital.The study was carried out on 177 hypertensive patients (with new onset of diabetes or without diabetes) visiting the OPD of medicine department at Majeedia hospital, New Delhi. The drug history of hypertensive patients and blood glucose levels following 1-5 yrs of antihypertensive therapy were recorded.The gender distribution of hypertensive patients reveals a higher percentage of incidences in males (53.7%) as compared to females (46.3%). Hypertensive patient without DM on beta blockers and on thiazide shows higher incidence of impaired glucose tolerance (IGT) (17.5%, 18.5%) and DM (10%, 11%) as compared to patient receiving other antihypertensive therapy. While in patients of new onset diabetes the incidence was higher with β-blockers (56.2%) than with thiazides (31.3%) followed by calcium channel blockers (CCBs) (12.5%). There was proportionate increase in incidence with the duration of therapy (3-5 years). None of the patients who were on ACE inhibitors or on angiotensin receptor blockers (ARBs) reported any incidence of IGT or DM.To conclude, β-blockers and thiazides increases the risk of type 2 diabetes mellitus with long term antihypertensive therapy requiring regular monitoring. CCBs have lowered risks while ACE inhibitors and ARBs are relatively free of such metabolic adverse effects., (© Georg Thieme Verlag KG Stuttgart · New York.)

Haemophilia B is caused by coagulation defects in the factor IX gene located in Xq27.1 on the X chromosome. Identification of mutations contributing to defective factor IX may be advantageous for precise carrier and prenatal diagnosis. We studied 16 patients from 11 families, consisting of 8 patients of the Malay ethnic group, of which 6 were siblings. Factor IX mutations have not been previously reported in the Malay ethnic group. The functional region of the factor IX gene was sequenced and mutations were identified in either the exon or intronic regions in 15 of the patients. One novel mutation, 6660&#95;6664delTTCTT was identified in siblings with moderate form of haemophilia B. Mutations identified in our patients when linked with disease severity were similar to findings in other populations. In summary, this preliminary data will be used to build a Malaysian mutation database which would facilitate genetic counseling.

Trichloroethylene (TCE) is a suspected genotoxic and carcinogenic compound which is usually present in the air, soil and water as pollutant. To estimate the genotoxic potential of TCE in a pure chemical form as well as an ingredient of the complex sample, Ames fluctuation test using TA98 and TA100 strains and Allium cepa genotoxicity assay were performed. For the genotoxicity analysis of TCE in natural milieu, the above mentioned tests were performed on the waste waters collected from two different stations of northern India namely Saharanpur and Aligarh, U.P., and these waste waters were supplemented with 50 and 100 mg/l of trichloroethylene. TCE alone was found to be non-genotoxic by both the testing system up to the range of 1000 mg/l concentration (data not shown). However, the test water samples supplemented with 100 mg/l of TCE, exhibited a significant increase in the genotoxicity compared with control by both the testing systems. In Ames fluctuation test, Mi(f) value was found to be increased by 41% and 53% with 100 mg/l of TCE supplemented Saharanpur and Aligarh waste water samples respectively, in the presence of S9 fraction compared with their respective controls. Allium cepa genotoxicity test also showed around 25% increase in total chromosomal aberration frequency following 100 mg/l TCE supplementation. However, supplementation of 50 mg/l TCE to the test water samples could not enhance the genotoxicity to a significant extent. From these results, we can conclude that TCE itself was non-genotoxic but it may promote mutation and/or DNA damage at a concentration of 100 mg/l under certain environmental conditions. We suggest that some chemicals in the test water samples might be interacting with TCE and/or metabolite(s) to cause the enhancement in genotoxicity. The mechanism of these synergistic effects should be explored further., (Copyright © 2011 Elsevier GmbH. All rights reserved.)

The work described here presents the toxic effect of Mathura refinery wastewater (MRWW) in plant (Allium cepa), bacterial (E. coli K12) and human (blood) system. The samples were collected from adjoining area of Mathura refinery, Dist. Mathura, U.P. (India). Chromosomal aberration test and micronucleus assay in (A. cepa) system, E. coli K12 survival assay as well as hemolysis assay in human blood were employed to assess the toxicity of MRWW. MRWW exposure resulted in the formation of micronuclei and bridges in chromosomes of A. cepa cells. A significant decline occurred in survival of DNA repair defective mutants of E. coli K12 exposed to MRWW. On incubation with MRWW, calf thymus DNA-EtBr fluorescence intensity decreased and percent hemolysis of human blood cells increased. An induction in the MDA levels of MRWW treated A. cepa roots indicated lipid peroxidation also. Collectively, the results demonstrate a significant genotoxic and cytotoxic potential of MRWW., (Copyright © 2011 Elsevier B.V. All rights reserved.)

Oxidative stress-mediated genotoxicity of wastewaters taken from two different cities, Saharanpur (SWW) and Aligarh (AWW), were compared with a battery of short-term assays namely the Allium cepa genotoxicity test, the plasmid-nicking assay, and the Ames fluctuation test. Both test-water samples - when used undiluted - increased the frequency of chromosomal abnormalities and/or micronuclei and alterations in the mitotic index of root cells of Allium cepa. Bridges and fragmentation of the chromosome were the predominant effects of the Saharanpur water sample while the Aligarh sample induced mainly chromosome fragmentation. Single- and double-strand breaks were also observed in plasmid DNA treated with these test wastewaters. The plasmid-nicking assay performed on SWW resulted in linearization of plasmid DNA when 18μl was tested (in a total reaction volume of 20μl). However, with the same amount of AWW, all three forms of plasmid, viz. supercoiled, open circular and linear were observed. Supplementation with specific scavengers of reactive oxygen species (ROS) caused a significant decline in mutagenicity of test-water samples in all the tests, pointing at oxidative stress as the mediator of the observed genotoxicity. The role of heavy metals in the AWW-induced oxidative stress and that of phenolics in SWW cannot be ruled out., (Copyright © 2011 Elsevier B.V. All rights reserved.)

Mutagenicity of wastewaters taken from two different cities was compared by means of Ames plate test and Ames fluctuation test. TA100 and TA98 strains of S. typhimurium exhibited the highest sensitivity against the Saharanpur sample (SWW) in terms of the slope (m) of the dose-response curve in the plate incorporation assay. However, the most sensitive strain against the test samples from Aligarh (AWW) was TA98. Interestingly, TA100 and TA98 strains also displayed the highest susceptibility towards the samples from Saharanpur in the fluctuation test. However, TA102 and TA100 responded maximally to AWW in this bioassay. Interestingly, S9 supplementation resulted in the decline in mutagenic potential of SWW contrary to significant increase with AWW by both the tests. Both samples were found to generate different types of ROS as predominant species. While SWW were shown to generate a high concentration of superoxide radicals and hydrogen peroxide, hydroxyl radicals were predominantly occurring in AWW. From our result, we conclude that both the test water samples were highly genotoxic. In view of the complementary nature of these two testing systems, we recommend both bioassays for the genotoxicity assessment of complex water samples., (2011 John Wiley & Sons, Ltd.)

The genotoxicity of certain water bodies was evaluated employing the DNA repair defective mutants of Escherichia coli, induction of prophage lamda in the lysogen and the plasmid nicking assay. All the test DNA repair defective mutants invariably exhibited more sensitivity than their isogenic wild-type strains but distinctive patterns against the three water samples viz. industrial waste water and the groundwater samples obtained from industrial estate of Aligarh as well as river water of Yamuna at Agra. A significant level of phage induction was also recorded in the test system exhibiting maximum induction in case of industrial waste water followed by that in river and groundwater samples, respectively. The single- and double-strand breaks were also observed in the plasmid DNA treated with industrial waste water and the river water samples. These findings are suggestive of the DNA damage induced by the test samples with the probable role of SOS repair in E. coli.

Plant-based bioassays have recently gained remarkable popularity among the toxicological/eco-toxicological assessment procedures. The reasons for their wide use are comparative simplicity, sensitivity, and cost-effectiveness as well as a good correlation with other toxicity tests. The present study describes the use of two plant bioassays, Allium cepa test and seed germination test in the evaluation of the toxicity/genotoxicity of industrial waste water and river water and standardization with the commonly occurring pollutants in Indian waters namely heavy metals and phenolics. Both tests were standardized to suit the Indian conditions, and the local varieties were used. Both bioassays responded significantly with the test range of heavy metals and phenolics. The toxicity of heavy metals was in the order of Cu > Ni > Cd in both the tests whereas 2,4-dinitrophenol was the most toxic among the phenolic compounds. Cabbage, millet, and cucumber, respectively, were found to be the most sensitive in the seed germination test for the test heavy metals and phenols. Significant amounts of chromosomal abnormalities including bridges, stickiness, and fragmentations were recorded with both the industrial waste water and the XAD concentrated river water samples by A. cepa test.