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This study reported a sexual assault case where Y-STR DNA profile was tested for the confirmation of male DNA. Within the DNA profile of the sample of accused, a tri-allelic pattern was detected at locus DYF387S1. Since this locus doesn't exist in any of the commercially available Y-STR amplification kits, So, the confirmation of this tri-allelic pattern was done by re-extracting and amplifying the extracted DNA by using Y-Filer Plus amplification kit (Thermo Fischer Scientific, CA, USA--Thermo) followed by separation of amplicons on Genetic Analyzer 3500xl.The allelic patterns of case sample belong to Type-2 pattern of tri-allelic outcome because peak heights were in balanced manner. This region is very much prone towards any structural mutation which could be either addition or deletion of allele, so for the forensic point of view this locus should be used carefully for kinship analysis. [ABSTRACT FROM AUTHOR]

We report here the first ever global study on genetic polymorphism using a Verifiler PlusTM autosomal STR multiplex system. The study evaluated genetic characteristics of 23 autosomal STRs in 200 unrelated residents of Guna district of Madhya Pradesh, India. Allele frequencies and forensic parameters are reported. Population comparison analysis was also performed using NJ tree and PCA plot. Penta E marker showed highest power of discrimination (0.938) among all 23 studied markers. The study also presents the first ever global forensic assessment in Indian population on D6S1043 marker (PD 0.937). The results demonstrated that all the 23 markers were highly polymorphic and the Verifiler PlusTM kit is suitable for forensic purposes in Indian population. [ABSTRACT FROM AUTHOR]

Objective: This study was planned to evaluate the genetic diversity in the admixed and Teli (a Hindu caste) populations of Maharashtra, India using 20 autosomal Short Tandem Repeat (STR) genetic markers. We further investigated the genetic relatedness of the studied populations with other Indian populations., Results: The studied populations showed a wide range of observed heterozygosity viz. 0.690 to 0.918 for the admixed population and 0.696 to 0.942 for the Teli population. This might be due to the multi-directional gene flow. The admixed and Teli populations also showed a high degree polymorphism which ranged from 0.652 to 0.903 and 0.644 to 0.902, respectively. Their combined value of matching probability for all the studied loci was 4.29 × 10 -25 and 5.01 × 10 -24 , respectively. The results of Neighbor-Joining tree and Principal Component Analysis showed that the studied populations clustered with the general populations of Jharkhand, UttarPradesh, Rajasthan and Central Indian States, as well as with the specific populations of Maharashtra (Konkanastha Brahmins) and Tamil Nadu (Kurmans). Overall, the obtained data showed a high degree of forensic efficacy and would be useful for forensic applications as well as genealogical studies.

We report here the first ever global study on genetic polymorphism using a Verifiler Plus TM autosomal STR multiplex system. The study evaluated genetic characteristics of 23 autosomal STRs in 200 unrelated residents of Guna district of Madhya Pradesh, India. Allele frequencies and forensic parameters are reported. Population comparison analysis was also performed using NJ tree and PCA plot. Penta E marker showed highest power of discrimination (0.938) among all 23 studied markers. The study also presents the first ever global forensic assessment in Indian population on D6S1043 marker (PD 0.937). The results demonstrated that all the 23 markers were highly polymorphic and the Verifiler Plus TM kit is suitable for forensic purposes in Indian population.