Search

Stories constitute a significant part of the Indian preschool curriculum due to their various benefits in preschool child development. However, the teachers' practice of stories and their perception regarding the influence of stories on preschool child development are vital determining factors in the benefit preschoolers receive from stories. The global concern for providing quality education for preschool children necessitates the exploration of teachers' practices and perceptions; however, such studies in the Indian context, where education is shaped by its multicultural and linguistic diversities, are limited. The present study provides an original contribution to exploring the Indian preschool teachers' practice of stories and perception of the influence of stories on preschool child development using a developed questionnaire, Teachers' Practice and Perception of Stories (TPPS). Ninety-six preschool teachers from English medium schools in Mangalore, India, participated in the study. The findings indicated that irrespective of teachers' demographic variables, they played an active role in telling stories to children and positively believed that stories facilitate speech and language, cognition, literacy, and socio-pragmatic development in preschoolers. The findings broadly apply stories for preschool child development in clinical and school settings.

Background: Pretend play is a form of play that involves nonliteral actions. There are limited studies reporting the developmental trends of pretend play behaviours of typically developing pre-schoolers. This knowledge would be beneficial in the early identification of deviations in pre-schoolers who have or are at risk of developing developmental disabilities. Aim: The present study aimed to describe the developmental trends in pretend play skills across different age groups of pre-schoolers. The study also aimed to understand the differential patterns in pretend play observed across the Free Play and Structured Toy Play scenarios. Setting: This study was conducted on pre-schoolers in a classroom of the school. Method: The study followed a cross-sectional study design. Forty-eight participants were recruited for the study and were divided into four groups. A video recording of the child's pretend play skills was recorded using a Sony-HDRCX405 camcorder in Free Play and Structured Toy Play scenarios. The recorded video was coded and analysed for the occurrence of pretend play skills using the Play in Early Childhood Evaluation System (PIECES) coding scheme. Results: The study results depict a developmental trend in the occurrences of pretend play skills in pre-schoolers. It also emphasises the importance of amalgamation of Structured Toy Play and Free Play scenarios for the child's holistic development because of the unique benefits of each scenario. Conclusion: The study findings could help in the formulation of Individualised Education Programme objectives for typically developing children and children with developmental disabilities involving play, thus enabling these children experience normalised, contextually pertinent experiences like their peers.

The lack of research exploring the influence of dynamic visual narratives on inference skills prompted the present study with an aim to profile the inference skills in school children between the ages of 6 years and 9 years 11 months using dynamic visual narratives. A total of 80 participants were considered for the study. An animated story was designed as the dynamic visual narrative stimuli based on which an "inference task" was formulated, which included forced-choice questions and free-response questions. The responses of the forced-choice questions were analyzed quantitatively using one-way analysis of variance (ANOVA), while the responses from the free-response questions were analyzed qualitatively. The results revealed an increase in inference skills with age, with children using less seductive details, beginning to display inductive inference, and providing multiple inferences. The inference profiles obtained from the study can serve as evidence for the development of bridging inference skills from dynamic visual narratives in children.

Little is explored regarding the modality-specific differences in recall abilities of preschool children. Understanding modality-specific differences in the recall at an early age might give an insight into age-linked trends, which can lay a foundation for later development. The current study used a cross-sectional design to investigate the modality-specific differences in recall abilities of preschool children using a story and story stimulus. A total of 20 typically developing children between the ages of 3.6 to 5.5 years were presented with a story followed by a story recall task across auditory, visual, and auditory-visual modalities to determine modality-specific recall abilities. The results were analyzed using mixed ANOVA, which revealed higher recall ability in the auditory-visual modality than in other modalities. The findings are explained based on Dual coding theory, Multimedia effect, Mental imagery skills, Visual superiority effect, Attentional behavior, and Comprehension skill. The study implies the importance of Auditory-Visual modality in facilitating better recall in story-based tasks among preschool children.

The study on "Spelling Ability of Students with Visual Impairment in Higher Education" was carried out with 30 samples enrolled in higher education. The samples were selected by using stratified sampling method. A standardized tool "Differential Aptitude Test" (1990) developed by George K. Bennett, Harold G. Seashore, and Alexander G. Wesman particularly the spelling test was adapted by the investigator was used for the collection of data. The independent variables of the study were Gender (Male & Female), Type of College (Government & Aided), Locality (Rural & Urban), Type of Family (Nuclear & Joint), Year and Level of Course (UG & PG), and Medium of Instruction (English & Tamil). The dependent variables included in the study were spelling ability. The data was collected by survey method. The result revealed that higher the qualification higher the spelling ability. So this study emphasis that importance need to be given in helping the visual impairment students to learn the concepts with spelling, which will pave the way for them to be independent in terms of learning in future.

Stories constitute a significant part of the Indian preschool curriculum due to their various benefits in preschool child development. However, the teachers' practice of stories and their perception regarding the influence of stories on preschool child development are vital determining factors in the benefit preschoolers receive from stories. The global concern for providing quality education for preschool children necessitates the exploration of teachers' practices and perceptions; however, such studies in the Indian context, where education is shaped by its multicultural and linguistic diversities, are limited. The present study provides an original contribution to exploring the Indian preschool teachers' practice of stories and perception of the influence of stories on preschool child development using a developed questionnaire, Teachers' Practice and Perception of Stories (TPPS). Ninety-six preschool teachers from English medium schools in Mangalore, India, participated in the study. The findings indicated that irrespective of teachers' demographic variables, they played an active role in telling stories to children and positively believed that stories facilitate speech and language, cognition, literacy, and socio-pragmatic development in preschoolers. The findings broadly apply stories for preschool child development in clinical and school settings. [ABSTRACT FROM AUTHOR]

Play is a universal code, which mirrors the all-round development of a child. Play development reflects the emergence of early cognitive skills in children. The current study profiles the developmental trends of different types of play during the free and structured play scenario in South India. The sample included 48 pre-school children, aged between 3.6 to 5.6 years. Children's play was recorded in two scenarios, which included free Play (spontaneous Play without any restrictions) and structured toy play (restriction of toy usage limited to the four types of toys provided by investigator), in the classroom settings. The data was analysed using the coding scheme that was adapted by combining two existing standardised play scales i.e. Play Observation Scale and Play in Early Childhood Evaluation System. In the coding scheme, play types were grouped under three domains which were Functional Play, Social Play, and Cognitive Play. Cognitive play was further divided into two domains that are Simple pretend play (SPP) and Complex pretend Play (CPP) which has the subtypes adapted from Play in Early Childhood Evaluation System. The results revealed that the occurrence of play type was higher in the free play scenario and reduced in the structured toy play scenario. A developmental trend was observed in free play over structured toy play among children. The findings would be helpful in planning playbased counselling/intervention strategies for children and create awareness to teachers about the various developmental patterns of play types. [ABSTRACT FROM AUTHOR]

Context: Female domestic workers (FDWs) comprise a significant part of the global workforce in informal sector. Nature of their workplace is such that the work goes unaccounted for in terms of employment policies or legislation. Aims: The aim of this study was to assess socio-demographic and occupational profile of FDWs. Subjects and Methods: A cross-sectional study was conducted in one ward of each of the five geographical zones of Pune city. Domestic workers employed in randomly selected residential societies therein were included in the study. Data collected by interview technique during house visit and general examination done. Statistical Analysis Used: Data were analyzed by using IBM SPSS 25.0 USA statistical software. Percentage, mean, and standard deviation were calculated. Results: Of the 573 FDWs, 62% were between 20 and 40 years, and 35% were educated up to middle school. Three-fourths were currently married. Fifty-one were sole breadwinners. Most FDWs had their own house with electricity and water supply. Almost half had been employed for 5--10 years, working in 3--4 households. Approximately 50% earned between Rs. 4000--8000 per month. Maximum received annual bonus. Conclusions: Working and living conditions of these FDWs are not as pitiful as depicted in previous studies. However, benefits accorded to the formal sector workers are lacking here like fixed days off, pension, and maternity leave. [ABSTRACT FROM AUTHOR]

Objective: To assess knowledge and practices related to menstruation and reproductive health amongst college going adolescent girls. Materials and Methods: Cross-sectional study done on 323 adolescent girls admitted in the year 2012 to professional colleges belonging to the health sciences faculty of a private university in Pune, India, using self-administered proforma. Results: Mean age of onset of menarche was 13.35 years. Many girls (86.65%) had knowledge of menstruation prior to menarche. For 68% of girls, mother was a source of menstrual information. Half of the girls reported some form of restriction in activities during menstruation due to religious reasons. 11% girls suffered from some form of reproductive tract infections (RTIs). Practices related to menstruation revealed that cloth piece is used for menstrual protection by 3% of girls. Soaked sanitary pads were disposed sanitarily by 96% of girls. Adolescent of medical faculty had significantly more knowledge than nursing faculty adolescents regarding emergency contraceptives (P < 0.05). Only four girls reported a history of sexual contact, of these, three were aware of emergency contraceptives, while one used them to prevent pregnancy. Conclusions: Adolescent girls received knowledge of menstruation prior to menarche from their mothers. Restriction in activity during menstruation due to religious reasons is practiced by many girls. Sanitary pad was used by almost all girls, and all disposed the same in a sanitary manner. More than half of the girls were aware about emergency contraceptives. Prevalence of RTI was found to be low and very few girls reported history of sexual exposure. [ABSTRACT FROM AUTHOR]

The effect of storage on the nutritional quality of sapota candy as influenced by different storage methods was studied. The selected candy samples were packed in three different packages viz. (metalized multilayered polyethylene) pouches, poly propylene pouches and poly ethene pouches and stored at ambient and refrigerated conditions. The candy prepared from 30/40/50°Brix sugar syrup dried at 60°C in tray dryer and stored in metalized pouches showed least changes in titratable acidity, ascorbic acid, total sugar, reducing sugar and non-reducing sugar. Sapota candy samples stored in metalized pouches were shelf stable for three months. [ABSTRACT FROM AUTHOR]

Background: This study aimed to assess the effectiveness of tuberculosis awareness program amongst school students. Methods: This school based interventional study was done on 135 secondary school students. They were randomly selected in field practice area of Urban Health Training Center (UHTC) of a private medical college in Pune city. Health awareness session on tuberculosis was conducted by using various visual and audiovisual aids by the medical college undergraduate students. A pre test and post proforma was filled before and after the session. Paired t test was used to assess the effectiveness of awareness program. Results: There was a highly significant (P = 0) increase in the knowledge of school students after the tuberculosis awareness program by medical undergraduate students. The mean pre test scores were 6.31 (52.58%), and the mean post test scores were 10.20 (85%). This activity also helped medical undergraduate students to acquire skills related to communication, working in a team, preparation and presentation of various visual aids for health awareness program; they also gained knowledge of research methodology. Conclusions: Health education program by medical students helped significantly to improve the knowledge of school students regarding tuberculosis. Thus, medical college students can be involved to some extent for conducting health related behavioral change communication (BCC) activities in schools during their Community Medicine morning posting. Collaboration of private medical colleges, schools, and district tuberculosis units (DTUs) can be ideally achieved under public private partnership (PPP) for health awareness programs. [ABSTRACT FROM AUTHOR]

The article discusses a research study which compared word retrieval abilities for nouns and verbs of young adults and the elderly in India. It describes the subject selection process and the computer software selected to present the experimental stimuli to participants. It is concluded that geriatric participants are slower than the younger group in word retrieval. A chart featuring the average latency time recorded for both groups is also given.

Objective The diagnosis of voice disorders is based on perceptual and acoustic paradigms. Modern acoustic analysis systems are relatively inexpensive and user friendly. One aspect of laryngeal function that is of great interest is the extent of vocal fold closure. Soft phonation index (SPI) is the parameter in Multi Dimensional Voice Program, which reflects the approximation of vocal folds. High values of SPI are stated to correlate with incomplete vocal fold adduction and are a better indicator of breathiness than EGG. This study aims to determine the sensitivity of this acoustic parameter of SPI, as a reflective indicator of incomplete vocal fold adduction in male patients diagnosed with unilateral vocal nodules. Methodology 60 participants were included in the study; 30 with normal vocal fold functioning in the control group and 30 with unilateral vocal nodules in the experimental group. The phonation sample of vowel/a/was recorded into CSL 4150 of Kay Elemetrics, in a sound treated room. The readings of SPI on the MDVP analysis was extracted and subjected to statistical analysis using independent samples 't-test' using SPSS Version 11. Results The results of the study reveal that there was a statistically significant difference between the means of the SPI values between the control and the experimental groups. Discussion It can be understood that SPI is a sensitive parameter to detect abnormalities in vocal fold approximation in the considered population of individuals with vocal nodules. This is in coherence with a study done on patients with vocal fold palsy and cordectomy, wherein SPI was found to be a good indicator of breathiness. The SPI mean obtained for participants in the control group was higher than the Western normative mean specified in MDVP, which highlights the importance of establishing normative values for Indian population. Conclusion It can be concluded that SPI was sensitive to detect changes affecting vocal fold closure in unilateral vocal nodules. But this conclusion has to be generalized with caution keeping in mind that only one population with vocal pathology was studied. The factors of vocal fold physiology and mechanical properties of vocal tract, which may contribute to individual variations in SPI values, have to be considered for further research. [ABSTRACT FROM AUTHOR]

Family Health Survey is introduced in the syllabus of Community Medicine to give adequate exposure to the undergraduate medical students about the family and its surrounding environment, so that they can understand the health needs of the community from a broader perspective.MBBS curriculum also have been made more community oriented by increasing learning hours in Community Medicine. Present study was planned to take feedback from students regarding family health survey after their community exposure. The study revealed that students received good community exposure and understood the life style, needs, and problems of people residing in the slums. Thus community oriented learning could be complementary to formal teaching in Community Medicine. [ABSTRACT FROM AUTHOR]

The article examines the perception of medical students at the department of Community Medicine of Bharati Vidyapeeth University Medical College (BVUMC) in Pune, India concerning the role of community based projects as a teaching tool. It notes that the BVUMC allows fourth to sixth semester undergraduate students to interact with various sections of the society through such projects. It mentions that 78% of the students expressed their satisfaction about the project while 96% agreed to have the project included in their curriculum.

Background: Considering the importance of exploring the development of reasoning skills during preschool period and the suitability of using a culturally linguistically relevant story-based approach for the same, the present research intended to profile the reasoning skills in typically developing Indian preschool children between 36 and 72 months using a story-based approach. The specific objectives were to determine the test-retest and inter-rater reliability of reasoning tasks within an existing story-based cognitive-communicative assessment tool and to use this tool to assess the reasoning skills of typically developing Indian preschool children. Method: Reasoning tasks across explanation, prediction and inference domains were evaluated for its psychometric properties and administered to 63 typically developing Indian preschool children attending English medium schools in Mangalore. The preschoolers were equally divided into three age groups. The responses obtained across the age groups were analyzed quantitatively and qualitatively. Results: The developed tasks were confirmed to have good psychometric properties like test-retest and inter-rater reliability. The age comparisons of reasoning abilities using one-way ANOVA suggested an increase in reasoning abilities with age during the preschool period. The qualitative analysis further suggested that with increasing age, the nature of reasoning changed from content-based reasoning to reasoning based on prior knowledge which was integrated with the story content. Conclusion: The study describes reasoning skill development using a story-based task in Indian preschoolers. The study findings further provide clinical and educational implications to assess and foster reasoning abilities among preschoolers., Competing Interests: No competing interests were disclosed., (Copyright: © 2024 Prasanna A et al.)

Purpose: The aim of the present study was to conceptualize and develop a novel context-based working memory (WM) task which would possess the potential to assess the context-based WM in an Indian scenario and incorporate a task-linked metacognitive facet to understand an individual's self-perception of performance., Methods: Employing the ADDIE instructional design model, comprising Analysis, Design, Development, Implementation and Evaluation phases, the present study focused on the initial four phases. In the Analysis-phase, the needs and objectives for creating a context-based WM task were identified. Design-phase included task conceptualization and content validation of the conceptualized context-based WM task. In the Develop-phase, the designed context-based WM task was developed in an animated video format. Five content experts and fifteen lay experts were involved in the content validation process. In the Implementation-phase, a pilot study was done on ten adults to assess the feasibility of the novel context-based WM task., Results: The task obtained good content validation index and understandability scores on the Patient Education Materials Assessment Tool. Preliminary data trends from the implementation-phase revealed the task's potential to detect age-related WM differences. Significantly correlated with established WM tasks, the novel Activity Ordering Task (AOT) effectively measured WM-spans. Observed discrepancies between performance and prediction/postdiction spans during metacognitive facet administration highlighted the AOT's utility in evaluating metacognition., Conclusion: Addressing the limitations of context in traditional tasks, the AOT appears to be a promising tool that not only measures WM but also assesses metacognition, thereby enhancing its utility beyond an everyday WM assessment.

Purpose: Prospective memory (PM) questionnaires are frequently used to evaluate perceptions of PM skills in daily life. This study aimed to systematically investigate communication-specific attributes using pre-existing PM self-rating questionnaires to inform clinicians and researchers about the role of PM in cognitive communicative evaluations., Methods: PM-related items from three questionnaires (i.e., Prospective Memory Questionnaire, Comprehensive Assessment of Prospective Memory, and Prospective and Retrospective Memory Questionnaire) were compiled and embedded in Google Forms and distributed to 70 Speech-Language Pathologists (SLPs) with expertise in Cognitive Communicative Disorders across India. Participants first identified items related to communication, and were then contacted to rate the communication-related PM items using a Likert scale for their degree of appropriateness. Responses from 40 SLPs were obtained and subjected to item-content validity index (i-CVI) and exploratory factor analysis (EFA)., Results: Of the 114 PM items, 28 received ratings over 50% for their relevance to communication. Of the 28 items, 21 had an i-CVI score greater than 0.8. After the removal of overlapping content, 14 items were finalized and subjected to EFA, which resulted in four factors: PM failure due to loss of communicative content, PM failure due to loss of communicative intent, PM cost due to ongoing interference, and PM failure linked to the priority of communicative intent., Conclusion: This study highlights communication-related aspects of PM that can be used as a framework for SLPs to assess and research PM skills.

Purpose: To study the awareness on mucormycosis among outpatients who visited six tertiary eye care hospitals at Madurai, Pondicherry, Coimbatore, Tirunelveli, Chennai, and Tirupati., Methods: This was a telephone-based survey conducted using questionnaires consisting of 38 questions in five sections from July 5 to 25, 2021. Patients visiting the eye hospitals for an examination were contacted over their phones and responses were directly entered onto the Google forms platform., Results: A total of 4573 participants were included in the study. Among all participants, a cumulative 83% of participants had some knowledge of mucormycosis. More than 80% of them reported that their prime source of information was through mass communication like television or radio. Around 34.8% of the respondents were aware that it can occur after treatment for coronavirus disease 2019 (COVID-19) infection, only half of them (54.3%) knew that systemic steroids were the main risk factor. The knowledge scores were higher for participants who were diabetics (n = 1235) or had been affected by COVID-19 earlier (n = 456) or whose friends had mucormycosis earlier (n = 312). Knowledge, attitude, and practice (KAP) scores of nonprofessional health-care workers (n = 103) were much better compared to patients., Conclusion: Such KAP studies give us an idea of the impact of the measures taken for educating the public. In this study, a cumulative 83% of participants had some knowledge of mucormycosis and 86% knew that this was an emergency. More than 50% of the participants were not aware that diabetes is a risk factor for mucormycosis., Competing Interests: None

Objective: The present study compared recognition of native and non-native consonants in quiet and noise among native speakers of Malayalam., Methods and Material: Fifteen native speakers of Malayalam who had English as the medium of instruction at school participated in the study. Stimuli comprised of 16 vowel-consonants-vowel nonsense syllables spoken by eight native speakers of Malayalam (native consonants) and eight native speakers of American English (non-native consonants). Recognition of native and non-native consonants was studied in quiet and in the presence of speech-shaped noise at signal-to-noise ratios (SNRs) of 8 dB, 0 dB, and -8 dB. The consonant recognition task was carried out as 16-alternative forced-choice procedure, and the responses were stored as confusion matrix., Results: In favourable listening condition (i.e., quiet and 8 dB SNR), the recognition score for native consonants was greater than non-native consonants. In contrast, at 0 dB SNR and -8 dB SNR, the recognition score of non-native consonants was greater than native consonants. Information transfer analysis revealed that the transfer of information was highest for consonant feature manner of articulation and lowest for voicing, across listening conditions for both native and non-native consonants., Conclusions: Recognition of native and non-native consonants were affected differently in the presence of speech-shaped noise among native speakers of Malayalam. In favourable listening condition, recognition of native consonants was better than non-native consonants. However, in challenging listening condition, non-native consonants were found to be recognised better than native consonants., Competing Interests: None

Coronary artery disease (CAD) has emerged as a major cause of morbidity and mortality worldwide. Recent findings on the role of genetic factors in the aetiopathology of CAD have implicated novel genes and variants in addition to those involved in lipid and lipoprotein metabolism. However, our present knowledge is limited due to lack of clarity on their exact identity and the quantum of impact on disease susceptibility, and incident risk. It is a matter of great interest to understand the role of genetic factors in ethnic populations that have a strong underlying predisposition to CAD such as the South Asian populations, particularly among Asian Indians living in India and abroad. Although, a number of isolated studies do implicate certain gene polymorphisms towards enhanced disease susceptibility, the available data remains scanty and inconclusive as they have not been validated in large, prospective cohorts. The present review aims to consolidate the available literature on the genetics of CAD in Asian Indians and seeks to provide insights on the concerns that need to be addressed in future studies to generate information having clinical value.

Objectives: Traditional measures of dysphonia were reported to be unreliable, and the measurements of cepstral peak prominence (CPP) have been shown to correlate well with perceptions of breathiness. Hence, the present study was hypothesized that there would be abnormal reduction of the cepstral peak in the speakers with vocal nodules relative to the normal controls as they are characterized by hoarse and breathy voice due to inadequate closure of vocal folds., Method: Phonation of sustained vowel /a/ was subjected to acoustic analysis using Computerized Speech Lab (CSL model 4150) software. The vowels were analyzed acoustically with the measurement of CPP., Results: Independent t test was employed to compare the significant differences between the clinical and the control groups in both males and females. The results revealed significant differences across the two groups at P<0.05. The obtained results were discussed with respect to the underlying pathophysiology., Conclusion: The present study investigated the CPP in subjects with vocal nodules. Cepstral deviations in the clinical group are explained due to the presence of laryngeal pathology leading to the lower values of CPP., (Copyright © 2010 The Voice Foundation. Published by Mosby, Inc. All rights reserved.)

Background: Roux-en-Y gastric bypass (RYGB) benefits patients with type 2 diabetes mellitus (T2DM) and a body mass index (BMI) >35 kg/m(2); however, its effectiveness in patients with T2DM and a BMI <35 kg/m(2) is unclear. Asian Indians have a high risk of T2DM and cardiovascular disease at relatively low BMI levels. We examined the safety and efficacy of RYGB in Asian Indian patients with T2DM and a BMI of 22-35 kg/m(2) in a tertiary care medical center., Methods: A total of 15 consecutive patients with T2DM and a BMI of 22-35 kg/m(2) underwent RYGB. The data were prospectively collected before surgery and at 1, 3, 6, and 9 months postoperatively., Results: Of the 15 patients, 8 were men and 7 were women (age 45.6 +/- 12 years). Their preoperative characteristics were BMI 28.9 +/- 4.0 kg/m(2), body weight 78.7 +/- 12.5 kg, waist circumference 100.2 +/- 6.8 cm, and duration of T2DM 8.7 +/- 5.3 years. At baseline, 80% of subjects required insulin, and 20% controlled their T2DM with oral hypoglycemic medication. The BMI decreased postoperatively by 20%, from 28.9 +/- 4.0 kg/m(2) to 23.0 +/- 3.6 kg/m(2) (P <.001). All antidiabetic medications were discontinued by 1 month after surgery in 80% of the subjects. At 3 months and thereafter, 100% were euglycemic and no longer required diabetes medication. The fasting blood glucose level decreased from 233 +/- 87 mg/dL to 89 +/- 12 mg/dL (P <.001), and the hemoglobin A1c decreased from 10.1% +/- 2.0% to 6.1% +/- 0.6% (P <.001). Their waist circumference, presence of dyslipidemia, and hypertension improved significantly. The predicted 10-year cardiovascular disease risk (calculated using the United Kingdom Prospective Diabetes Study equations) decreased substantially for fatal and nonfatal coronary heart disease and stroke. No mortality, major surgical morbidity, or excessive weight loss occurred., Conclusion: RYGB safely and effectively eliminated T2DM in Asian Indians with a BMI <35 kg/m(2). Larger, longer term studies are needed to confirm this benefit., (Copyright 2010 American Society for Metabolic and Bariatric Surgery. Published by Elsevier Inc. All rights reserved.)

Natural infection of feral Indian rhesus monkeys (Macaca mulatta) by a new simian beta retrovirus, provisionally called simian retrovirus-7 (SRV-7) is described. The virus is capable of in vitro replication in primary human peripheral blood lymphocytes (PBL) and B and T cell lines. We have earlier reported a novel SRV, SRV-6 from Indian langurs (Semnopithecus entellus). Additional sequence analyses from gp20 transmembrane (TM) env genes of SRV-6 and SRV-7 place them in a separate cluster, related to but distinct from known exogenous SRVs and also close to the simian endogenous beta retrovirus, (SERV) from African baboon. Phylogenetic analyses of pol gene of SRV-7 place it closer to SERV when the stop codons of the SERV genes are removed. On the other hand, additional sequence data from gp70, surface glycoprotein (SU) region of the env gene of SRV-6 suggest it is more closely related to known exogenous SRVs, (SRV-1 to 3). It is also related to the endogenous langur virus, Po-1-Lu. We hypothesize that SRV-6 and SRV-7 probably originated from a progenitor exogenous SRV which recombined with an endogenous SERV in the TM env and pol genes during evolution, based on the phylogenetic analyses.

We have previously reported natural infection of Hanuman langurs (Semnopithecus entellus) from Lucknow, India by a novel simian retrovirus, SRV-6, a beta-retrovirus (type D retrovirus). Here we describe infection by a closely related SRV-6 in an isolated feral population of Hanuman langurs from Jodhpur in the Northwestern desert region of India. Serological analyses, using in-house ELISA and WB, genomic amplification, and sequencing of env region (gp70 and gp20) of the viral genome were carried out. SRV-6-infected langurs from the two regions were serologically cross-reactive. The env gene was used for phylogenetic analyses, being the most variable part of a retroviral genome. The surface glycoproteins (gp70) were almost identical between the two SRV-6 isolates and related to but distinct from equivalent regions from other exogenous SRVs. We could sequence the transmembrane glycoprotein gp20 from SRV-6 infecting the Jodhpur langurs, which was again shown to be related to but unique compared to the other known SRVs. The study suggests that natural infection by related strains of SRV-6 occurs in wild langurs from different parts of India.

The role of enteric-type adenoviruses and rotaviruses in mild and severe acute gastroenteritis was investigated among children younger than 5 years of age seeking treatment at an urban hospital (UH) and at a rural health center (RHC) in India. There were 330 children at the UH and 340 at the RHC; 319 and 315 age matched nondiarrheal children served as controls for the respective groups. Rotavirus was detected in 15.2% of 330 cases and 1.9% of 319 controls at the UH (P less than 0.001) and in 16.5% of 340 cases and 2.9% of 315 controls at the RHC (P less than 0.001). RV excretion was 3- to 5-fold more common in severe compared with mild diarrhea at the UH and at the RHC (P less than 0.001). The detection rate for enteric-type adenoviruses was similar in patients and controls, respectively, at the UH (0.9%; 2.5%) and RHC (3.8%; 2.5%). At the RHC adenovirus types other than 40 and 41 were excreted by 8.8% of the patients and by only 1.0% of the controls (P less than 0.001). It is possible that the diarrheagenic role of adenoviruses may not be restricted to types 40 and 41.

A rural cohort of 507 children, less than 3 yr of age was kept under weekly surveillance for 12 months, for morbidity due to diarrhoea. Stool specimens for rotavirus detection were obtained in 346 of the 360 diarrheal episodes occurring in 354 children and in 211 children without diarrhoeal symptoms. Rotavirus was detected by an ELISA test in 4 per cent of children with diarrhoea and 6.6 per cent of control subjects. Viral genome RNA was extracted by phenol chloroform method from all the positive samples and analysed by polyacrylamide gel electrophoresis followed by silver staining. All the strains isolated, exhibited the same electropherotype with a 'long' RNA migration pattern. This result contrasts with the genomic variability amongst rotavirus strains usually observed in different settings.

Serum anti-gliadin antibody (AGA) titres were estimated by diffusion in a gel enzyme-linked immunosorbent assay in children with coeliac disease (n = 11), protracted diarrhoea of non-coeliac causes (n = 110), acute gastroenteritis (n = 20), protein energy malnutrition (n = 20), and asymptomatic, well-nourished children (n = 66). The mean IgG and IgA AGA titres were significantly higher (p less than 0.001) in children with coeliac disease than in any other groups. There was no significant difference (p greater than 0.01) in AGA titres in relation to age, nutritional status, or severity of villous injury. In patients with coeliac disease AGA titres showed a good correlation with disease activity. The specificity and sensitivity of the assay are discussed.

Among 274 neonates born at the maternity services of an urban hospital in India, 36.1% of the infants shed rotavirus in feces (as detected by ELISA) by 72 h of life. The excretion rate increased to 70.3% among the 120 infants who stayed for 5 days or more at the hospital. Diarrhoeal symptoms of mild and self-limited nature were observed only in 19.2% of the rotavirus excretors, the remaining being asymptomatic. Among the 98 infants who received supplement feeds, 49% acquired rotavirus infection as against 24.7% of the 150 exclusively breast fed infants (p less than 0.001). Viral RNA in the feces of all rota positive infants showed the same electropherotype, indicating infection from a common source. The mean percentage rotavirus inhibitory activity of cord sera in the infected and non-infected infants was 50.2 +/- 21.7 and 56.6 +/- 19.2 respectively (p greater than 0.05), suggesting that cord blood antibodies do not offer significant protection against neonatal rotavirus infection.