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Mothbeanis a drought and heat tolerant pulse crop grown in arid and semi-arid regions of India. The extent of genetic variability, correlation, path analysis for seed yield and nine yield attributes was studied in thirty-eight genotypes during the kharif, 2019 in randomized complete block design in four replications. The differences among all the genotypes were significant for all the traits. Days to flowering recorded high genotypic and phenotypic coefficient of variation followed by plant height and low magnitudes were reported for protein content, pod length and the number of seeds per pod. The broad sense heritability was high for days to flowering, days to maturity, the number of pods per plant, hundred seed weight, plant height, seed yield per plant, the number of branches per plant and the number of seeds per pod. A low genetic advance as per cent mean was recorded for protein content and pod length. The trait, seed yield per plant was significantly and positively correlated with the number of pods per plant at both genotypic (0.662) and phenotypic (0.618) levels. The path analysis revealed that the number of pods per plant, days to maturity, the number of seeds per pod and plant height exhibited a high direct effect on seed yield per plant. The microsatellites were used to analyse the polymorphisms among mothbean genotypes. The polymorphic information content value of the microsatellites ranged from 0.23 to 0.57, while the heterozygosity value ranged from 0.26 to 0.64. The cluster diagram revealed a similarity index value ranged from 0.08 to 1.00 and the genotypes were clustered in two main groups. Based on the results it could be concluded that microsatellites are efficient in discriminating among the mothbean genotypes and the study showed a narrow gene base of the genotypes. [ABSTRACT FROM AUTHOR]

The members of the genus Asota are widely distributed from Africa, India, Sri Lanka, Myanmar, and Malayan regions to the Australian region containing 55 described species. Asota ficus (Fabricius, 1775) is one among the nine species of the genus described from India having a wide range of distribution. The present study includes the first mitochondrial DNA barcode generated from India for A. ficus with a valid voucher describing external morphological characters together with the male and female genitalia. Discussions pertain to the utility of DNA barcodes for studies on moths in India with a comment on the identity of other sequences showing shallow genetic divergence with our sequences. [ABSTRACT FROM AUTHOR]

Introduction: Myxozoan infections of Indian freshwater fishes are relatively well studied, but their validity is supported with molecular and phylogenetic data only for a few species., Objectives: The primary objective was to present molecular data for two Myxozoan species, Myxobolus cylindricus and Henneguya gachua collected from Indian freshwater fishes, the dwarf snakehead Channa gachua and the striped dwarf catfish Mystus vittatus, respectively., Methods: Various organs of 56 C. gachua and 48 M. vittatus were dissected. Myxozoan plasmodia with mature spores were collected from the gills under a dissecting microscope. Spores obtained from plasmodia from infected hosts were fixed in 80% ethanol in vials and sent for further morphological and molecular examinations to Hungary. The 18S rDNA gene of Myxobolus and Henneguya spp. was amplified using nested polymerase chain reaction (PCR). Phylogenetic analysis was performed using maximum likelihood (ML) and Bayesian inference (BI) methods., Results: Morphological characteristics of M. cylindricus and H. mystasi spores corresponded to the original descriptions made by Sarkar, Mazumdar and Pramanik, 1985 and Haldar, Samal, and Mukhopadhyay, 1997, respectively. Phylogenetic analysis of the 18S rDNA gene revealed that the sequences of M. cylindricus differed from those of most Indian Myxobolus sp., known mostly from cyprinid fishes and formed a subgroup with Myxobolus neurophilus, a parasite of a perciform host, and with Henneguya chaudhuryi, a species belonging to a different genus but described from a closely related channid host. It was also closely related to another Henneguya species, H. lesteri, described from Sillago analis, a coastal fish. Henneguya mystasi had the closest similarity to Henneguya bicaudi, a species described from an Indian cyprinid fish and to Henneguya pellucida reported from a characid fish known from South America., Conclusion: Molecular data received by us gives a solid basis for further identification of these myxozoans, the pathogenicity of which probably plays an economic role at culturing the hosts.