1. Impact of interleukin-6 promoter polymorphism and serum interleukin-6 level on the acute inflammation and neovascularization stages of patients with Eales' disease.
- Author
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Sen A, Paine SK, Chowdhury IH, Mukherjee A, Choudhuri S, Saha A, Mandal LK, and Bhattacharya B
- Subjects
- Acute Disease, Adult, Aged, C-Reactive Protein analysis, C-Reactive Protein biosynthesis, Case-Control Studies, DNA Fingerprinting, Enzyme-Linked Immunosorbent Assay, Female, Humans, India, Inflammation complications, Inflammation epidemiology, Inflammation immunology, Interleukin-6 immunology, Male, Middle Aged, Neovascularization, Pathologic complications, Neovascularization, Pathologic epidemiology, Neovascularization, Pathologic immunology, Promoter Regions, Genetic, Retinal Perforations genetics, Retinal Perforations immunology, Retinal Vasculitis complications, Retinal Vasculitis epidemiology, Retinal Vasculitis immunology, Vascular Endothelial Growth Factor A genetics, Vascular Endothelial Growth Factor A immunology, Vitreous Body chemistry, Inflammation genetics, Interleukin-6 genetics, Neovascularization, Pathologic genetics, Polymorphism, Single Nucleotide, Retinal Vasculitis genetics, Vitreous Body immunology
- Abstract
Purpose: To evaluate the role of interleukin-6 (IL-6) in the inflammatory and proliferative stages of Eales' disease (ED) and to determine the influence of IL-6-174G/C polymorphism in the IL-6 and IL-6-regulated protein expression, as well as the development of ED., Methods: One hundred and twenty-one patients diagnosed with ED, 223 matched healthy controls, and 16 control patients with macular holes were recruited from the eastern Indian population. Serum and vitreous levels of IL-6 and vascular endothelial growth factors (VEGF) were measured by enzyme-linked immunosorbent assay. Serum levels of high-sensitivity C-reactive protein (hsCRP) were measured by enzyme immunoassay. Subjects were genotyped for the IL-6-174G/C polymorphism (rs1800795) by a custom TaqMan single-nucleotide polymorphism (SNP) Genotyping Assays system., Results: Serum IL-6 (p<0.0001), hsCRP (p<0.0001), and VEGF (p=0.0031) levels were significantly higher in the inflammatory stage of ED than in healthy controls. Serum IL-6 also significantly correlated with hsCRP (Spearman's correlation coefficient; r=0.4992, p=0.0009), but not with VEGF in this stage in ED patients. At the proliferative stage of ED, significantly higher levels of vitreous IL-6 (p=<0.0001) and VEGF (p=<0.0001) were found compared with the vitreous of patients with macular holes. A significant correlation was observed between vitreous IL-6 and VEGF in ED patients (Spearman's correlation coefficient; r=0.5834, p=0.0087). A statistically significant association was found between the -174GG genotype (p=0.006) and occurrence of ED. Mean serum and vitreous concentrations of IL-6 were also higher in the subjects with the GG genotype than in those with the GC or CC genotype in this population., Conclusions: IL-6 expression, regulated by the allelic distribution of -174 loci and the enhanced level of IL-6, modulates CRP and VEGF concentration depending respectively on the acute inflammatory stimulation at the initial stage and angiogenic stimulation at the advanced stage of ED.
- Published
- 2011