KUMAR, VIPUL, KOSHIAK, MANEESHA, KUMAR, DEVENDERA, ARCHANA, T. S., SHARMA, HIMANSHI, and SINGH, SHIVAM
A new strain of Trichoderma virens was isolated from mushroom compost which causes green mold disease from the mushroom production unit of Lovely Professional University, Punjab, India. Based on 5.8S rRNA, partial 18S, and 28S rRNA genes, ITS1, ITS4 sequence analysis, and morphological characteristics, the fungal isolate was identified as T. virens. The universal primers (ITS-1 and ITS-4) were used to amplify the 28S rRNA gene fragment that produced a sharp band of about 600 bp on the agarose gel. The amplified gene fragment was then sequenced and showed 100% similarity with T. virens. GC/MS analysis of the strain revealed the presence of several antimicrobial compounds, such as n-nonadecanol-, propanoic acid, 2-methyl-butanoic acid, and 2-methyl-dodecane. The current study confirmed the potent antimicrobial and chitinolytic activity against Fusarium and its potential as a bio-control agent for managing plant diseases. [ABSTRACT FROM AUTHOR]