1. Complete genome sequence of a new bipartite begomovirus infecting Boehmeria leiophylla in China.
- Author
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Zhao L, Zhong J, Zhang X, Ding M, and Zhang Z
- Subjects
- Base Sequence, Begomovirus isolation & purification, China, DNA, Viral genetics, India, Phylogeny, Plant Diseases virology, Whole Genome Sequencing, Begomovirus genetics, Boehmeria virology, Genome, Viral, Sequence Analysis, DNA
- Abstract
A bipartite begomovirus was identified from a Boehmeria leiophylla plant sample exhibiting yellow mosaic symptoms collected in Nabanhe National Nature Reserve, Xishuangbanna, Yunnan, China. Five complete DNA-A and four DNA-B genome sequences were obtained by rolling-circle amplification (RCA), cloned, and sequenced. All DNA-A sequences were determined to be 2759 nucleotides in size, sharing 99.9%-100% nucleotide sequence identity with each other. The DNA-B sequences were comprised of 2673 nucleotides, sharing 98.6-100% nucleotide sequence identity with each other. Genomic organization of the begomovirus was typical of Old World bipartite begomoviruses. Sequence analysis revealed 81.84% nucleotide sequence identity to tomato leaf curl Hsinchu virus (ToLCHsV) from China for the DNA A component and 67.23% identity to the squash leaf curl China virus (SLCCNV) from India for the DNA B component. The sequence comparisons suggest that this bipartite begomovirus represents a novel species for which we propose the name "Ramie yellow mosaic virus".
- Published
- 2018
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