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Background: One of the common reasons for implantation failure is a thick or hard zona pellucida. This is due to biochemical changes in the zona pellucida caused by a variety of factors, including advanced maternal age, in-vitro embryo culture conditions, and cryopreservation, which may impair in-vivo hatching and implantation after thawing and transfer. As a result, laser assisted hatching (LAH) has been promoted as a method of accelerating implantation and assisting the natural hatching process. Objective: The primary goal of this research is to assess the effect of laser-assisted hatching (LAH) on implantation and clinical pregnancy rates in patients undergoing fertility treatment at a tertiary-level infertility clinic. The secondary goal is to assess the rate of multiple pregnancy and miscarriages following the transfer of Day 5 frozenthawed embryos in women aged 21 to 43 years. Materials and methods: This study includes a total of 492 patients, ages 21 to 43, with a self-stimulating cycle who underwent an all-freeze procedure. The whole population is divided into two groups: the LAH and non-LAH groups, each containing 169 patients. The frozen Day 5 embryos of the LAH group were thawed and subjected to LAH during embryo transfer. The effect of LAH on primary outcomes such as implantation and pregnancy rates and secondary outcomes such as multiple pregnancy and miscarriage rates was compared in both the LAH and non-LAH groups. Both groups were further subdivided into two groups: patients in Group I were under the age of 35 years, while those in Group II were over the age of 35 years. Results: According to the study's findings, patients in the LAH group under the age of 35 have 70% implantation rates and better pregnancy outcomes than those over the age of 35. It was also discovered that LAH significantly improved clinical pregnancy rates in both young (P < 0.001) and older (P < 0.03) patients when compared to the non-LAH group. In addition, the patient's age and the number of day-5 frozen-thawed embryos transferred during embryo transfer influenced pregnancy rates (P = 0.01) in the LAH group versus the non-LAH group. Conclusion: To overcome the negative effect of zona hardening in Indian population, LAH can routinely be offered for frozen day 5 embryos after thawing in FET cycles to increase the pregnancy rate without increasing the overall rate of early miscarriages and multiple pregnancy. [ABSTRACT FROM AUTHOR]