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This article focuses on the Asplanchna and the spines of Brachionus calyciflorus in two Javanese sewage ponds. A substance liberated by Asplanchna causes females of Brachionus calyciflorus to produce offspring with postero-lateral spines. In plankton samples from two sewage ponds near Bandung in Java the postero-lateral spines of B. calyciflorus were extraordinarily long. The Javanese sewage ponds are extremely rich in zooplankton, and the density of Asplanchna is very high. The relationship between postero-lateral spine length and Asplanchna density is also confirmed by a comparison of the two ponds.