1. Analysis and evaluation of the antimicrobial and anticancer activities of the essential oil isolated from Foeniculum vulgare from Hamedan, Iran.
- Author
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Akhbari M, Kord R, Jafari Nodooshan S, and Hamedi S
- Subjects
- Allylbenzene Derivatives, Animals, Anisoles analysis, Artemia drug effects, Cell Line, Tumor, Gas Chromatography-Mass Spectrometry methods, Gram-Negative Bacteria drug effects, Humans, Iran, Oils, Volatile analysis, Oils, Volatile isolation & purification, Plant Oils analysis, Plant Oils pharmacology, Toxicity Tests, Anti-Bacterial Agents pharmacology, Antineoplastic Agents, Phytogenic pharmacology, Foeniculum chemistry, Oils, Volatile pharmacology
- Abstract
In this study, biological properties of the essential oil isolated from seeds of Foeniculum vulgare ( F. vulgare ) were evaluated. GC-MS analysis revealed Trans-Anethole (80.63%), L-Fenchone (11.57%), Estragole (3.67%) and Limonene (2.68%) were the major compounds of the essential oil. Antibacterial activity of the essential oil against nine Gram-positive and Gram-negative strains was studied using disc diffusion and micro-well dilution assays. Essential oil exhibited the antibacterial activity against three Gram-negative strains of Pseudomonas aeruginosa , Escherichia coli , and Shigella dysenteriae . The preliminary study on toxicity of seed oil was performed using Brine Shrimp lethality test (BSLT). Results indicated the high toxicity effect of essential oil (LC50 = 10 μg/mL). In vitro anticancer activity of seed oil was investigated against human breast cancer (MDA-Mb) and cervical epithelioid carcinoma (Hela) cell lines by MTT assay. Results showed the seed oil behave as a very potent anticancer agent with IC50 of lower than 10 μg/mL in both cases.
- Published
- 2019
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