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Background: Cutaneous leishmaniasis (CL) is an endemic infection in the Middle East, including Iran that is also spreading to new foci. We aimed to determine the leishmaniasis species causing CL in Alborz province. Methods: Overall, out of 55-suspected CL patients referred to health centers in Alborz Province, north central Iran in 2019, 40 patients had positive smear for CL based on optical microscopy. The internal transcribed spacer 1 (ITS1) of nuclear ribosomal DNA (rDNA) was amplified by PCR. Leishmania species were identified by PCR-restriction fragment length polymorphism (PCR-RFLP) using BshF I (Hae III) enzyme. Results: Out of the 40 positive patients with CL, 34 cases (85%) had been caused by Leishmania (L) major and six (15%) by L. tropica. Fifteen patients had no history of traveling to the disease endemic areas, of which nine were Iranians. Skin lesions and scars caused by CL were mostly observed on the hands and face. Moreover, more than two skin lesions were observed in 22 cases (55%), all of which were infected with L. major. A single skin ulcer was seen in 18 (45%) of the CL patients. Conclusion: Climate change, reduced rainfall, and demographic changes such as migration into Alborz Province and the increasing marginalization of the population and their entry to settle in new areas might have caused natural transmission of both L. tropica and L. major in this province. [ABSTRACT FROM AUTHOR]

Visceral leishmaniasis (VL) is a neglected disease. Our retrospective study describes 38 clinical and epidemiological characteristics of VL in patients admitted to a paediatric hospital in Tehran, Iran, who came from different geographical regions, indicating that the disease has spread to most parts of the country. Some 76.3% of the children documented suffered with symptoms of the disease for two months before admission. [ABSTRACT FROM AUTHOR]

Abstract- Squamous-cell carcinoma (SCC) of the eye conjunctiva is a rare tumor. Its link with immune impairment suggests that infectious agents such as human papillomavirus (HPV) may be involved in the etiology of SCC. We conducted a case--control study on 50 SCC cases (mean age: 65.2) and 50 age frequency-matched control patients with lesion-free, normal conjunctival biopsies (mean age: 63.8) obtained from the cancer registry archive at Pathology Department of Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran, Iran, where SCC has become the most common conjunctival malignancy. MY/GP nested PCR was performed for HPV detection and E6/E7 consensus primers in combination of type specific primers were used in another nested PCR series for HPV typing. HPV DNA was detected in 46 of 50 samples of squamous cell carcinoma and none of the normal biopsies by nested PCR using primer sets of the HPV consensus L1 region (MY/GP). Subsequently, specimens from the 46 positive cases were subjected to specific PCR. Although 630bp amplicon was produced in 44 of 46 samples (E6/E7 primers), none of the specific HPV PCR reactions for HPV DNA type 16, 18, 31 or 33 resulted in the detection of HPV DNA in the 44 SCC specimens of the conjunctiva. Current results confirm the role of HPV in the etiology of conjunctival SCC. The absence of HPV 16, 18, 31 and 33 in conjunctival SCC in this study raise doubts about the role of genital types of HPV in conjunctival carcinomas. [ABSTRACT FROM AUTHOR]

• There were adverse effects on RBC, WBC, AST, ALT and LDH. • Serum levels of FSH, LH and testosterone were lower in rams held in closer proximity and/or on the downwind side of the smelter. • Expression of HSD17B3 and CYP11A1 genes decreased in the proximity of smelter. Detrimental effects of heavy metals on animals and humans are of considerable concern. This research describes the long-term effects of exposure of male sheep to emissions from a copper smelter. Blood parameters and reproductive hormones were measured in male Kermani sheep reared around a copper smelter in KhatoonAbad, Kerman province, Iran. Representative blood samples were collected from randomly-selected rams (3–4 years old) at 4 geographical directions (south, north, east and west) and 4 distances (approximately 10, 20, 30 and 40 km) from the smelter. Hematological and biochemical parameters including WBC, HCT, HGB, AST, ALT, ALP and LDH were higher (P < 0.05) in flocks located at 10 km south from the smelter and lower at 40 km west; indicating liver and kidney damage in the sheep reared at shorter distances to the smelter. The relative expressions of HSD17B3 and CYP11A1 mRNA levels in the testis were lower at 10 km and highest at 40 km distance. Expression levels of HSD17B3 and CYP11A1 in the west direction were 11.3 and 11.1 percent higher than in the south direction. The lowest concentration of FSH, LH and T, were observed at 10 km south, an indication of deranged hypothalamic- hypophyseal- testicular function in these sheep. At 10 km distance, FSH concentration was 70 percent lower, and at 10 km south, T and LH levels were 92 and 83 percent lower than the highest values obtained at 40 km west. It was concluded that long-term exposure to heavy metals around this smelter may reduce the general health and normal reproductive function in male sheep, and probably other living species. However, more detailed studies in the region are warranted. [ABSTRACT FROM AUTHOR]

Purpose : The current study was undertaken to determine the frequency of specific primary conjunctival neoplastic lesions in a patient population treated at an ocular service over a 15-year period. Materials and Methods : We analyzed the data of all primary cases of conjunctival neoplasia diagnosed from 1990 to 2004 in the pathology department of Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran, Iran. Results : Of 3,242 conjunctival specimens, 447 were identified as primary conjunctival neoplasms. The patient group comprised 270 (60.4%) males and 177 (39.6%) females, with an age range of 1 to 90 years. The mean age was 38 years and the median was 32 years. The most common primary conjunctival neoplastic lesion was nevus (38.7%), followed by squamous cell carcinoma, (25.1%), papilloma (7.8%), dysplasia (5.6%), and hemangioma (5.1%), respectively. Of 447 lesions, 261 (58.4%) were benign, 134 (30%) were malignant, and 52 (11.6%) were precancerous. Conclusion : As in previously published studies, benign lesions were the most frequent, but the percent of malignant lesions was much higher than that described in other reports. The high percentage of squamous cell carcinoma that we observed can likely be attributed to elevated sun exposure and ultraviolet light in Iran. The characterization of precancerous lesions in this study emphasizes their potential to transform into malignant lesions and the need for sufficient early management and follow up. [ABSTRACT FROM AUTHOR]

Purpose: The present study aimed to compare the effectiveness of music and playing with Lego in postoperative management pain in children., Design and Methods: In this three-group quasi-experimental study, the participants in this interventional study were 96 children aged 6 to 12 years admitted to the pediatric surgery ward of Mofid Hospital and Medical Center in Tehran. The participants were selected using convenience sampling. They were then randomly placed into three intervention groups. Pain intensity was measured for the participants in all three groups before the intervention. The intervention was performed in two 15-min sessions with an interval of 5 min in three intervention groups. Then pain intensity was measured using the Oucher Pain Scale before the intervention and in four intervals: Immediately after the intervention, half an hour, one hour, and 3 h after the intervention, respectively. Pain intensity was also measured at the same time intervals for the participants in the control group. The collected data were analyzed with SPSS-25 software using descriptive statistics, the Bonferroni test, and Tukey's test., Results: The data showed that the three groups were identical in terms of demographic characteristics (p > 0.05).A comparison of the music listening group and the control group showed statistically significant difference in terms of postoperative pain (P < 0.05).However, the data showed significant differences between the control group and the Lego group in terms of pain intensity immediately after the intervention, half an hour, one hour, and 3 h after the intervention, respectively (p < 0.05).The data also revealed a significant difference between the children in the music group and the Lego group at all phases (p < 0.05), and the children in the Lego group reported less pain after the intervention compared to the children in the music listening group. Nevertheless, there was a statistically significant difference between the three groups in terms of reported pain intensity half an hour, one hour, and three hours after the intervention (p < 0.05)., Conclusion: The results showed that playing with Legos had a greater effect on reducing postoperative pain in children than listening to music. Thus, nurses are recommended to make arrangements for children to play with Legos after surgery., Practice Implication: Playing with Legos can be used to control postoperative pain in school-age children in medical centers and wards of pediatric hospitals., Competing Interests: Declaration of Competing Interest There are no conflicts of interest., (Copyright © 2022 Elsevier Inc. All rights reserved.)