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Chicken infectious anemia virus (CIAV) is an immunosuppressive viral disease causing high economic losses in poultry industry. In this study, 20 farms were represented for a prevalence study of the disease in Ismailia governorate, Egypt.ON532690.1 and ON532691.1 Isolates from bone marrow, thymus loops, liver, and spleen of broiler farms exhibiting some clinical and postmortem signs were used for reverse transcription polymerase chain reaction assay. RT-PCR was used to amplify a 418bp product of the CIAV VP1 gene. Three farms out of 20 (15%) were positive. Phylogenetic tree of partial vp1 amino acids were classified into three groups according to change in H/22/N-Q amino acid indicated that there are three CIAV different strains circulating in Egypt. Hematological investigation revealed significant decrease in RBCs count, hemoglobin concentration, and packed cell volume declared normocytic normochromic anemia. The immunological studies revealed a significant decrease in serum lysozyme, nitric oxide (NO), antioxidants (CAT and GSH), total protein, and in the majority of serum protein fractions in infected chickens (G2) compared to apparently healthy (G1) while there were marked increase in G2 than G1 in A: Gratio. This result guides to review the vaccination programs against CIAV in Egypt for improving the immune response against the infection. [ABSTRACT FROM AUTHOR]