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Introduction: We have recently developed a new miniaturized device for extracorporeal ultrafiltration (UF) to be used in patients with fluid overload: Artificial Diuresis-1 (AD1) (Medica S.p.A., Medolla, Italy). The device has a reduced priming volume, operates at very low pressures and flow regimes, and is designed to perform extracorporeal UF at bedside. After accurate experiments were carried out in vitro, we report in this paper the results of in vivo UF sessions carried out in selected animals according to veterinary best practice., Materials and Methods: The AD1 kit is pre-filled with sterile isotonic solution and operates with a polysulfone mini-filter, MediSulfone (polysulfone at 50,000 Dalton). A collection bag with a volumetric scale is connected to the UF line, and the ultrafiltrate is obtained by gravity based on the height at which the ultrafiltrate collection bag is placed. Animals were prepared and anesthetized. The jugular vein was cannulated with a double-lumen catheter. Three 6-h sessions of UF were scheduled with a target fluid removal of 1,500 mL. Heparin was used as anticoagulant., Results: In all treatments, the target value of UF was obtained in the absence of major clinical or technical problems with a maximum deviation from the scheduled UF rate lower than 10%. The device resulted to be safe, reliable, accurate, and easily usable thanks to a user-friendly interface and its very small dimensions., Conclusions: This study opens the way for clinical trials in different settings including departments with low intensity of care and even in ambulatory centers or patient's home., (© 2023 The Author(s). Published by S. Karger AG, Basel.)

The impact of climate change could undermine the future grain production as a consequence of increased temperature and drought condition or improve the crop performance owing to the increased CO 2 in the atmosphere. Wheat water demand and yield are strictly related to climate conditions of the area where the plants are cropped. In this study, we assessed the future trends of grain yield and water consumption in two European regions, Germany (Continental region) and Italy (Mediterranean region) in the light of the multiple sources of uncertainty related to climate and yield forecasts. Four crop models were set up under combinations of two European climate regions, five Global Circulation Models and two Representative CO 2 Concentration Pathways, 486 ppm and 540 ppm in 2050. Yield and water use were assessed under rainfed and irrigated regimes, and the water footprint of green water and total water was estimated. Our results indicated that projected yields were comparable (Mediterranean area) or even improved (+9%; Continental area) in rainfed conditions in comparison to the current trend; and water supply enhanced crop performance (+22% in Germany and +19% in Italy, as mean). Crop water consumption (both green and blue) remained stable in future projections but the water footprint was 5% lower on average in Italy and 23% in Germany when compared to the baseline. Despite the uncertainty in future predictions related to the factors analysed, our result indicated that current wheat production and its water footprint could become more favourable under climate change., (Copyright © 2018. Published by Elsevier B.V.)

The evaluation of sperm functionality and morphology allows discerning between high and low quality ejaculates, but does not give detailed predictive information regarding in vivo fertility. The current developments in statistical modeling have helped in carrying out reproductive studies, but their biggest limitation is in the size of the dataset to be used. The aim of the present observational study was to evaluate whether advanced statistical approaches, such as mixed effects regression models and bootstrap resampling, can help in assessing the predictive ability of semen parameters in terms of in vivo fertility (farrowing rate and litter size), on a small/medium farm with a limited number of animals. Data regarding 33 ejaculates, including viability, subjective motility and acrosome reaction, were collected. Two hundred and thirty-five sows were inseminated with an outcome of 167 deliveries and 1734 newborn piglets. In order to evaluate the relationships among the parameters measured and fertility, mixed effects regression statistical models were used. Once the covariates to be included in the final models were identified, non-parametric bootstrapping was used. The results showed that the farrowing rate was highly associated with the total number of spermatozoa and subjective motility, while litter size was associated with percentage of acrosome reaction. In conclusion, the proposed statistical approach seemed to be suitable for studies regarding reproduction and fertility, even for relatively small sample sizes. Nonetheless, larger data sets are still preferable and required in order to achieve higher reliability., (Copyright © 2018 Elsevier B.V. All rights reserved.)