Search

y.-s. chen, f. yanagida and t. shinohara. 2005.To survey, and identify and classify the ecological distribution of lactic acid bacteria from soil in Japan and Taiwan.Acid-producing bacteria were isolated from 68 soil samples, collected from Japan and Taiwan, in the rhizospheres of fruit trees, from the floor of a henhouse and around a horse farm. All isolates were identified by physiological and genetic tests. Thirty-two of the 54 isolates were identified as lactic acid bacteria (LAB), 16 as spore-forming lactic acid bacteria, five asClostridiumand one asBacillus. These lactic acid bacteria represent five genera:Lactobacillus,Lactococcus,Enterococcus,LeuconostocandWeissella.A high rate of isolating lactic acid bacteria was obtained from soil.This study suggests that soil may be a common source for the isolation of lactic acid bacteria. [ABSTRACT FROM AUTHOR]

Aims: To isolate, characterize and identify lactic acid bacteria (LAB) in the vineyards where koshu grapes, a primary wine grape cultivar in Japan, are grown., Methods and Results: Sixty samples, including leaves, undamaged grape berries and soil under damaged berries, were collected at four koshu vineyards in Yamanashi Prefecture, Japan. One hundred and 15 acid-producing cultures were isolated from these samples, and the isolates were divided into classes by phenotype and then into groups by restriction fragment length polymorphism analysis and sequencing of 16S ribosomal DNA (rDNA). Phenotypic and biochemical characteristics identified seven different bacterial groups (A to G). Lactococcus lactis ssp. lactis was the most abundant type of LAB distributed in three koshu vineyards, and Leuconostoc pseudomesenteroides was the most abundant LAB found in the remaining vineyard. Forty-six isolates produced bacteriocin-like inhibitory substances (BLIS) against the indicator strain Lactobacillus sakei JCM 1157(T)., Conclusions: These results suggest that various LAB are distributed in koshu vineyards, of which a large number produce BLIS., Significance and Impact of the Study: This is the first report describing the distribution and varieties of LAB that exist in koshu vineyards.