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1. Development of SCAR markers and PCR assays for single or simultaneous species-specific detection of Phytophthora nicotianae and Pythium helicoides in ebb-and-flow irrigated kalanchoe

Author

Ahonsi, Monday O., Ling, Yin, and Kageyama, Koji

Subjects
*POLYMERASE chain reaction, *MICROBIOLOGICAL assay, *BIOMARKERS, *PHYTOPHTHORA nicotianae, *PYTHIUM, *OOMYCETES, *PATHOGENIC microorganisms, *KALANCHOE
Abstract

Abstract: Phytophthora nicotianae and Pythium helicoides are important water-borne oomycete pathogens of irrigated ornamentals particularly ebb-and-flow irrigated kalanchoe in Japan. We developed novel PCR-based sequence characterized amplified region markers and assays for rapid identification and species-specific detection of both pathogens in separate PCR reactions or simultaneously in a duplex PCR. [ABSTRACT FROM AUTHOR]

Published
2010
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