1. Increased phenolic content and antioxidant capacity of the heated leaves of yacon (Smallanthus sonchifolius).
- Author
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Ueda, Yuto, Matsuda, Yasushi, Murata, Tatsuro, Hoshi, Yoshikazu, Kabata, Kiyotaka, Ono, Masateru, Kinoshita, Hideki, Igoshi, Keiji, and Yasuda, Shin
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YACON , *OXIDANT status , *HIGH performance liquid chromatography , *CHLOROGENIC acid , *CAFFEIC acid , *PHORBOL esters , *PLANT phenols - Abstract
We investigated the content of phenolic compounds and antioxidant capacity of two batches of non-heated and heated leaves of the yacon cultivar "Andes no yuki", grown in Japan. Lyophilized yacon leaves heated at 160°C for 20 min and 100°C for 60 min had a 1.96 to 9.69-times higher total phenolic content than that of the non-heated leaves. Heated leaves exhibited a 1.98 to 4.07-times higher antioxidant capacity than that of the non-heated leaves in three different free radical scavenging assays. Heated leaves were more efficient at attenuating the superoxide anion radical production in human granulocytic cells than the non-heated leaves. High-performance liquid chromatography analysis revealed that, in the heated leaves, the caffeic acid content was 2.13 to 3.64-times higher and the chlorogenic acid content was slightly lower than those in the non-heated leaves. Hence, heat processing may affect the active constituent contents in yacon leaves, potentiating its antioxidant capacity. Abbreviations: ABTS+: 2,2′-azinobis(2-ethylbenzothiazoline-6-sulfonic acid) cation; DPPH: 1,1-diphenyl-2-picrylhydrazyl, HPLC: high-performance liquid chromatography; NBT: nitroblue tetrazolium; O2−: superoxide anion; PMA: phorbol 12-myristate 13-acetate; PMS: phenazine methosulfate; TEAC: Trolox equivalent antioxidant capacity Outline of increased phenolic content and antioxidant capacity of the heated leaves of yacon (Smallanthus sonchifolius). [ABSTRACT FROM AUTHOR]
- Published
- 2019
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