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Objective-To retrospect the construction and application of the dual referral system for infertility treatment in Jiangsu Province, China. With the standard procedure to screen infertile pathogeny, those doctors of reproductive medicine with formal training can diagnose and treat patients step by step, and smoothly transfer treatment between superior and subordinate reproductive centers. Methods:The standardized diagnosis and treatment procedure of infertility and sterility and the clinical pathway of preliminary screening on the pathogeny were established. The database system, CCRM, was upgraded in the practice in our center. The advanced training course of 3 months was designed for doctors. The expert out-calls and lecture tours were scheduled in Jiangsu Province. The clinical shortcut service system, so called the Green Channel, and the demonstration clinics were well developed. Results : There were 211 309 patients undergone the preliminary screening system from January 2005 to October 2013 in our center. 21 hospitals joined the program. 1 149 patients underwent the transfer treatment, the referral rate is 2.6% (1 149/211 309). The cost of the preliminary screening on pathogeny was 1 116.4 RMB. Times of the expert out-call were 14,and 1 185 patients were diagnosed in those expert out-calls. 236 trainees finished their three-months' advanced training program; 2 529 participants finished training in 28 courses. 40 outpatient clinics of infertility were set up and 56 hospitals joined in the training of the database system. Conclusions-It is concluded from our practice that the infertility dual referral system was effectively developed in Jiangsu Province, China, and that the standardized infertility diagnosis and treatment procedure was also successfully developed. With above system and procedure and the clinical pathway of preliminary screening on the pathogeny of infertility, it is feasible to reduce the medical cost for patients, and to provide the high-quality medical service in the limited times of visiting doctors. [ABSTRACT FROM AUTHOR]